Results 51 to 60 of about 1,066,757 (310)
Primer Extension Analysis of HBV DNA with Strand-Specific Primers
BIO-PROTOCOL, 2015 We performed primer extension assay to determine which steps of HBV DNA synthesis (i.e., minus- and plus-strand DNA synthesis and circularization of RC DNA) are affected by phosphoacceptor site mutations in C protein. In these experiments, we used several specific oligonucleotide primers.
Jaesung Jung, Kyongmin Kim
openaire +2 more sources
Reciprocal control of viral infection and phosphoinositide dynamics
FEBS Letters, EarlyView.Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley +1 more source
Monitoring otter populations by DNA typing of spraints [PDF]
, 2001 To monitor mammals by direct observation is often very difficult. Therefore a new technique based on DNA typing of droppings has been developed. DNA typing of otter spraints can potentially provide estimates of population size, home ranges, dispersal ...
Jansman, H.A.H. +2 more
core
Pilot progress in DNA isolation and amplification from the material stored at the LWS herbarium
Інтродукція РослинThe isolation of DNA from the herbarium specimens deposited at the LWS herbarium (State Museum of Natural History of the NAS of Ukraine, Lviv, Ukraine) has been tested using the column-based protocol.
Andriy Novikov, Viktor Nachychko
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Identifi cation of Salmonella spp and serovars Typhimurium, Enteritidis by qPCR
Науковий вісник ветеринарної медицини, 2020 This article presents the results of the identifi cation of the Salmonella genus as well as serovars Enteritidis and Typhimurium by a real-time polymerase chain reaction.
Rublenko N.
doaj +1 more source
Eliminating mitochondrial DNA competition for nuclear DNA primers. [PDF]
Genome Research, 1993 Mitochondrial DNA (mtDNA) sequences were synthesized with nuclear DNA (nucDNA) sequence-tagged site (STS) primers by mismatch priming in three independent studies of the human nuclear genome. Mismatch primer binding sites on the mtDNA were identified with from 6- to 10-bp identity at the 3' ends of the primers.
S, Zullo +8 more
openaire +2 more sources
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Detection of Phytophthora species by MALDI-TOF mass spectrometry [PDF]
, 2005 Phytophthora diseases have caused worldwide economic, social and environmental impacts for decades. Once their presence is confirmed, they are difficult to eradicate.
Siricord, Cornelia Charito
core +1 more source
Primers Used for DNA Sequence Analysis. [PDF]
, 2015 Note. Primers were HPLC purified, 50 nmoles from InvitrogenPrimers Used for DNA Sequence Analysis.
Erin Stokes (696333) +4 more
core +1 more source