Results 61 to 70 of about 2,585,724 (312)

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

open access: yesFEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Identifi cation of Salmonella spp and serovars Typhimurium, Enteritidis by qPCR

open access: yesНауковий вісник ветеринарної медицини, 2020
This article presents the results of the identifi cation of the Salmonella genus as well as serovars Enteritidis and Typhimurium by a real-time polymerase chain reaction.
Rublenko N.
doaj   +1 more source

Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples [PDF]

open access: yes, 2011
Large-scale and in-depth characterization of the intestinal microbiota necessitates application of high-throughput 16S rRNA gene-based technologies, such as barcoded pyrosequencing and phylogenetic microarray analysis.
Vos, W.M., de   +9 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Detection of Leaf Blotches - Causal Agents in Barley Leaves and Grains

open access: yesCzech Journal of Genetics and Plant Breeding, 2004
The objective of this paper was to adapt PCR-based detection method for R. secalis and P. teres DNA isolated from pathogens and also from artificially infected juvenile leaves and seeds using pathogen-specific primers.
J. Gubiš   +5 more
doaj   +1 more source

Primer Extension Analysis of HBV DNA with Strand-Specific Primers

open access: yesBIO-PROTOCOL, 2015
We performed primer extension assay to determine which steps of HBV DNA synthesis (i.e., minus- and plus-strand DNA synthesis and circularization of RC DNA) are affected by phosphoacceptor site mutations in C protein. In these experiments, we used several specific oligonucleotide primers.
Jaesung Jung, Kyongmin Kim
openaire   +2 more sources

Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for detection of Leishmania DNA in buffy coat from visceral leishmaniasis patients [PDF]

open access: yes, 2012
BACKGROUND: Visceral leishmaniasis (VL) remains as one of the most neglected tropical diseases with over 60% of the world's total VL cases occurring in the Indian subcontinent.
Mondal, D.   +23 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

DNA Metabarcoding Using Indexed Primers: Workflow to Characterize Bacteria, Fungi, Plants, and Arthropods from Environmental Samples

open access: yesDiversity
Environmental DNA from bulk materials can be analyzed to gain an understanding of the bacterial, fungal, plant, and/or arthropod communities present. DNA metabarcoding is widely used to characterize these biological communities, by amplifying “barcode ...
Teresa M. Tiedge   +2 more
doaj   +1 more source

Development of a Species Diagnostic Molecular Tool for an Invasive Pest, Mythimna loreyi, Using LAMP

open access: yesInsects, 2020
The Mythimna loreyi (Duponchel) is one of the well-known invasive noctuid pests in Africa, Australia, and many Asian countries. However, it is difficult to identify the invasive and morphologically similar species, Mythimna separate, which occur at the ...
Hwa Yeun Nam   +3 more
doaj   +1 more source

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