Results 61 to 70 of about 1,480,527 (305)

Development of a Species Diagnostic Molecular Tool for an Invasive Pest, Mythimna loreyi, Using LAMP

Insects, 2020
The Mythimna loreyi (Duponchel) is one of the well-known invasive noctuid pests in Africa, Australia, and many Asian countries. However, it is difficult to identify the invasive and morphologically similar species, Mythimna separate, which occur at the ...
Hwa Yeun Nam, Min Kwon, Hyun Ju Kim, Juil Kim   +3 more
doaj   +1 more source

Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]

, 2018
In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini, Campanaro, Stefano, Kougias, Panagiotis G., Treu, Laura, Zhu, Xinyu   +4 more
core   +3 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Development of A PCR - Cycle Sequencing System for Animal Mitochondrial DNA Analysis [PDF]

, 1994
A system for the study of sequence polymorphisms in animal mitochondrial DNA was developed. This system consists of two candidate markers which are mitochondrial cytochrome b and D-loop.
Lau, Chin Hoon
core  

Fetal Brain Tumor Harboring a Unique ROCK1::BRAF Fusion

Pediatric Blood &Cancer, EarlyView.
Marllon Cindra Sant'Ana, Mariana Maschietto, Daniela Paz Leal, Ricardo de Santos de Oliveira, Mayara Ferreira Euzébio, Antonio Carlos dos Santos, Luciano Neder, Carlos Alberto Scrideli, Elvis Terci Valera   +8 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Development of PCR Blocking Primers Enabling DNA Metabarcoding Analysis of Dietary Composition in Hematophagous Sea Lamprey

Ecology and Evolution
Conventional dietary assessments are challenging in hematophagous species, particularly in sea lamprey (Petromyzon marinus). However, recent technological developments and molecular approaches have provided an attractive alternative through the use of ...
Conor O'Kane, Nicholas S. Johnson, Kim T. Scribner, Jeannette Kanefsky, Weiming Li, John D. Robinson   +5 more
doaj   +1 more source

Analisis DNA Bacillus SP. BAC4 Hasil Polymerase Chain Reaction (PCR) Dengan Primer-Primer Yang Dirancang Dari Gen Pga [PDF]

, 2008
Bacillus sp. BAC4 DNA was amplified using PCR method employing primers designed from pga genes of 5 bacteria (Arthrobacter viscosus, Bacillus megaterium, Escherichia coli, Kluyvera citrophila, and Providentia rettgeri).
Moeis, M. R. (Maelita), Onggowidjaja, P. (Philips), Ratnaningsih, E. (Enny)   +2 more
core  

Identification of type A and B isolates of Epstein-Barr virus by polymerase chain reaction [PDF]

, 1990
A method is described for the identification of type A and type B isolates of Epstein-Barr virus (EBV) by means of the polymerase chain reaction. The use of three pairs of primers specific for genomic sequences coding for the two forms of EBV nuclear ...
Alliger, P., Jilg, Wolfgang, Siegert, E., Wolf, Hans J.   +3 more
core   +1 more source

Primer Extension Analysis of HBV DNA with Strand-Specific Primers

BIO-PROTOCOL, 2015
We performed primer extension assay to determine which steps of HBV DNA synthesis (i.e., minus- and plus-strand DNA synthesis and circularization of RC DNA) are affected by phosphoacceptor site mutations in C protein. In these experiments, we used several specific oligonucleotide primers.
Jaesung Jung, Kyongmin Kim
openaire   +2 more sources