Results 71 to 80 of about 1,480,527 (305)
Microarray-based DNA resequencing using 3′ blocked primers [PDF]
Analytical Biochemistry, 2008 To exceed the throughput and accuracy of conventional sequencing technologies, we tested a method (pyrophosphorolysis-activated polymerization [PAP]) of nucleic acid amplification that uses 3' blocked primers (P*s). As proof-of-principle, we resequenced a 20-bp region of the factor IX gene with a microarray of P*s.
Jakub, Sram, Steve S, Sommer, Qiang, Liu
openaire +2 more sources
Structural biology of ferritin nanocages
FEBS Letters, EarlyView.Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley +1 more source
Neoplasia: An International Journal for Oncology Research, 2018 Excess telomere shortening has been observed in most cancer cells. The telomere quantitative polymerase chain reaction (qPCR) assay has become an important tool for epidemiological studies examining the effects of aging, stress, and other factors on the ...
Paige N. Dahlgren +4 more
doaj +1 more source
Squalen, 2020 Seafood-borne outbreaks caused by Vibrio cholerae have led to the increased need for food safety risk assessment of marine products. An in silico investigation about the potential of virulence gene of V.
Stalis Norma Ethica +6 more
doaj +1 more source
Mutually Uncorrelated Primers for DNA-Based Data Storage
, 2017 We introduce the notion of weakly mutually uncorrelated (WMU) sequences, motivated by applications in DNA-based data storage systems and for synchronization of communication devices.
Gabrys, Ryan +3 more
core +1 more source
Attempts to detect retrotransposition and de novo deletion of Alus and other dispersed repeats at specific loci in the human genome [PDF]
, 2001 Dispersed repeat elements contribute to genome instability by de novo insertion and unequal recombination between repeats. To study the dynamics of these processes, we have developed single DNA molecule approaches to detect de novo insertions at a single
AJ Jeffreys +14 more
core +1 more source
Self-Reporting PNA/DNA Primers for PCR Analysis [PDF]
Genome Research, 2001 We report a new fluorogenic method for sealed-tube PCR analysis using a quencher-labeled peptide nucleic acid (Q-PNA) probe. The Q-PNA hybridizes to a complementary tag sequence located at the 5′ end of a 5′ fluorophore-labeled oligonucleotide primer, quenching the primer's fluorescence. Incorporation of the primer into a doublestranded amplicon causes
M J, Fiandaca +3 more
openaire +2 more sources
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source
Journal of Hymenoptera Research, 2018 Archival specimens are a great resource for molecular research in population biology, taxonomy and conservation. A primary goal for researchers is to preserve specimens from collections by improving noninvasive methods for DNA extraction and to achieve ...
Milana Mitrović, Željko Tomanović
doaj +3 more sources
Designing of primer for PCR [PDF]
, 2003 The polymerase chain reaction (PCR) is the technique for in vitro synthesis of multiple copies of a given DNA molecule. Primers are the most important components of PCR and success of PCR largely depends on the primers. A primer is an oligonucleotide. It
Thomas, P C
core