Results 71 to 80 of about 2,987,670 (314)

Primers for DNA barcode generation and Bar-HRM analysis.

open access: yes, 2022
Primers for DNA barcode generation and Bar-HRM analysis.
Vipawee Pichetkun (12850839)   +5 more
core   +1 more source

Development of PCR Blocking Primers Enabling DNA Metabarcoding Analysis of Dietary Composition in Hematophagous Sea Lamprey

open access: yesEcology and Evolution
Conventional dietary assessments are challenging in hematophagous species, particularly in sea lamprey (Petromyzon marinus). However, recent technological developments and molecular approaches have provided an attractive alternative through the use of ...
Conor O'Kane   +5 more
doaj   +1 more source

Effectiveness of annealing blocking primers versus restriction enzymes for characterization of generalist diets: unexpected prey revealed in the gut contents of two coral reef fish species. [PDF]

open access: yesPLoS ONE, 2013
Characterization of predator-prey interactions is challenging as researchers have to rely on indirect methods that can be costly, biased and too imprecise to elucidate the complexity of food webs.
Matthieu Leray   +3 more
doaj   +1 more source

From junk to function — How weak selection in eukaryotes builds new parts and drives genomic complexity

open access: yesFEBS Letters, EarlyView.
How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley   +1 more source

Development of a New Monochrome Multiplex qPCR Method for Relative Telomere Length Measurement in Cancer

open access: yesNeoplasia: An International Journal for Oncology Research, 2018
Excess telomere shortening has been observed in most cancer cells. The telomere quantitative polymerase chain reaction (qPCR) assay has become an important tool for epidemiological studies examining the effects of aging, stress, and other factors on the ...
Paige N. Dahlgren   +4 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

DNA primers used for RT-PCR.

open access: yes, 2013
DNA primers used for RT-PCR.
Robert N. Fariss (105726)   +4 more
core   +1 more source

New internal primers targeting short fragments of the mitochondrial COI region for archival specimens from the subfamily Aphidiinae (Hymenoptera, Braconidae)

open access: yesJournal of Hymenoptera Research, 2018
Archival specimens are a great resource for molecular research in population biology, taxonomy and conservation. A primary goal for researchers is to preserve specimens from collections by improving noninvasive methods for DNA extraction and to achieve ...
Milana Mitrović, Željko Tomanović
doaj   +3 more sources

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

DNA primers used for site-directed mutagenesis.

open access: yes, 2016
DNA primers used for site-directed mutagenesis.
Ruiping Cai (3161421)   +6 more
core   +1 more source

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