T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis
Biotechnology & Biotechnological Equipment, 2016 Site-directed mutagenesis (SDM) is a useful tool to study the functions of regulatory sequences of DNA and RNA, and the structures and functions of proteins. Numerous methods have been developed for either single or multiple SDM (MSDM).
Wei Guo +4 more
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A spontaneous termination mechanism of RNA polymerase V shapes the DNA methylation landscape in plants [PDF]
The EMBO JournalDNA methylation plays critical roles in eukaryotic gene silencing, genome imprinting, viral defense, and suppression of transposable elements. In plants, RNA Polymerase V (Pol V)-generated non-coding RNA guides DNA methylation through the RNA-directed ...
Guohui Xie +15 more
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RNA INTERFERNCE (RNAI) FOR SOME GENES FROM BABESIA BOVIS [PDF]
Research Journal of Applied Biotechnology, 2016 Babesia parasites are responsible for enormous economic losses to the livestock industry worldwide. There is no vaccine available to protect from the infection.
Ikuo Igarashi +2 more
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Single-molecule view of coordination in a multi-functional DNA polymerase
eLife, 2021 Replication and repair of genomic DNA requires the actions of multiple enzymatic functions that must be coordinated in order to ensure efficient and accurate product formation.
Raymond F Pauszek III +3 more
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RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
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SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. [PDF]
PLoS Genetics, 2011 DNA methylation is an evolutionarily conserved epigenetic modification that is critical for gene silencing and the maintenance of genome integrity. In Arabidopsis thaliana, the de novo DNA methyltransferase, domains rearranged methyltransferase 2 (DRM2),
Julie A Law +3 more
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ESTABLISHMENT OF A RAPID SCREENING METHOD FOR IN VITRO SITE-DIRECTED MUTANTS OR CRISPR/Cas9-MEDIA-TED KNOCK-IN MUTANTS [PDF]
精准医学杂志, 2023 Objective To establish a rapid screening method for in vitro site-directed mutants or CRISPR/Cas9-mediated knock-in mutants, and to improve mutant screening efficiency and reduce experimental costs.
SONG Chunlin, ZHANG Yuqing, ZHAI Yujing, ZHANG Gang, WANG Ying
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Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. [PDF]
PLoS Genetics, 2008 DNA methylation directed by 24-nucleotide small RNAs involves the small RNA-binding protein ARGONAUTE4 (AGO4), and it was previously shown that AGO4 localizes to nucleolus-adjacent Cajal bodies, sites of snRNP complex maturation. Here we demonstrate that
Carey F Li +5 more
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Animals, 2023 The genus Capripoxvirus belongs to the Poxviridae family. The sheeppox, goatpox, and lumpy skin disease viruses are three species of this genus with 96% identity in their genomes.
Ghulam Mustafa +6 more
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HemaSphere, 2022 Resistance to cytarabine is a key problem in the treatment of acute myeloid leukemia (AML). To understand the molecular biology of resistance to cytarabine, a viability-based chemosensitizer screen was utilized. We screened synthetic lethal targets using
Miriam Rehberger +7 more
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