Amplification of 4–9-kb Human Genomic DNA Flanking a Known Site Using a Panhandle PCR Variant
BioTechniques, 1997 We present a method for the in vitro amplification of >6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted repeat of a portion of the known sequence, followed by single-primer polymerase chain ...
Douglas H. Jones, Stanley C. Winistorfer
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The ability to form homodimers is essential for RDM1 to function in RNA-directed DNA methylation. [PDF]
PLoS ONE, 2014 RDM1 (RNA-DIRECTED DNA METHYLATION1) is a small plant-specific protein required for RNA-directed DNA methylation (RdDM). RDM1 interacts with RNA polymerase II (Pol II), ARGONAUTE4 (AGO4), and the de novo DNA methyltransferase DOMAINS REARRANGED ...
Taku Sasaki +4 more
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tRNATrp as primer for RNA-directed DNA polymerase: structural determinants of function.
Journal of Biological Chemistry, 1979 The specific interactions between the RNA-directed DNA polymerase of avian oncornavirus and the tRNATrp primer required for initiation of viral DNA synthesis in vitro were examined.
B. Cordell +3 more
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Frontiers in Microbiology, 2016 Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme ...
Yi-Ping eWang +6 more
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Inhibitors of HIV-1 Reverse Transcriptase—Associated Ribonuclease H Activity
Biology, 2012 HIV-1 enzyme reverse transcriptase (RT) is a major target for antiviral drug development, with over half of current FDA-approved therapeutics against HIV infection targeting the DNA polymerase activity of this enzyme. HIV-1 RT is a multifunctional enzyme
Michael A. Parniak +4 more
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Simultaneous Disruption of Two DNA Polymerases, Pol eta and Pol zeta, in Avian DT40 Cells Unmasks the Role of Pol eta in Cellular Response to Various DNA Lesions [PDF]
, 2010 Replicative DNA polymerases are frequently stalled by DNA lesions. The resulting replication blockage is released by homologous recombination (HR) and translesion DNA synthesis (TLS). TLS employs specialized TLS polymerases to bypass DNA lesions.
Sonoda, E +44 more
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eLife, 2022 In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer +5 more
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UV- and MMS-induced mutagenesis of lambdaO(am)8 phage under nonpermissive conditions for phage DNA replication. [PDF]
, 2003 Mutagenesis in Escherichia coli, a subject of many years of study is considered to be related to DNA replication. DNA lesions nonrepaired by the error-free nucleotide excision repair (NER), base excision repair (BER) and recombination repair (RR), stop ...
Felczak, Magdalena +3 more
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Visualizing DNA polymerase ι catalyze Hoogsteen-directed DNA synthesis
Nature CommunicationsTranslesion synthesis polymerases efficiently incorporate nucleotides opposite DNA lesions. Pol ι, for example, bypasses minor-groove and exocyclic purine adducts.
Zach Frevert +6 more
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The Inhibitory Action of Kohamaic Acid A Derivatives on Mammalian DNA Polymerase β
Molecules, 2008 We previously isolated a novel natural product, designated kohamaic acid A (KA-A, compound 1), as an inhibitor of the first cleavage of fertilized sea urchin eggs, and found that this compound could selectively inhibit the activities of mammalian DNA ...
Yoshiyuki Mizushina +9 more
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