Optimization of Gene Design, PCR Assembly and Site-Directed Mutagenesis for the Synthesis of Beauveria Bassiana Protease Gene [PDF]
Journal of Sciences, Islamic Republic of Iran, 2021 Synthetic gene construction is one of the components of synthetic biology. It can be used for various purposes such as to optimize gene expression.
Muhammad Azharuddin Azali +4 more
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Role of RNA Interference (RNAi) in the Moss Physcomitrella patens [PDF]
, 2013 RNA interference (RNAi) is a mechanism that regulates genes by either transcriptional (TGS) or posttranscriptional gene silencing (PTGS), required for genome maintenance and proper development of an organism.
Arif, Muhammad Asif +2 more
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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]
, 2012 Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu, Huanting, Naismith, James Henderson
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Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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Bridge helix bending promotes RNA polymerase II backtracking through a critical and conserved threonine residue. [PDF]
, 2016 The dynamics of the RNA polymerase II (Pol II) backtracking process is poorly understood. We built a Markov State Model from extensive molecular dynamics simulations to identify metastable intermediate states and the dynamics of backtracking at atomistic
Da, Lin-Tai +7 more
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RNase-like domain in DNA-directed RNA polymerase II. [PDF]
Proceedings of the National Academy of Sciences, 1991 DNA-directed RNA polymerase is responsible for gene expression. Despite its importance, many details of its function and higher-order structure still remain unknown. We report here a local sequence similarity between the second largest subunit of RNA polymerase II and bacterial RNases Ba (barnase), Bi, and St. The most remarkable similarity is that the
T, Shirai, M, Go
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Mechanisms of PARP inhibitor resistance in cancer and insights into the DNA damage response
Genome Medicine, 2018 Editorial summary Inhibitors of poly(ADP-ribose) polymerase (PARPi) have entered the clinic for the treatment of patients with cancers that lack homology-directed DNA repair, but drug resistance remains a clinical hurdle.
Paola Francica, Sven Rottenberg
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New link between the RNA polymerase II-CTD and replication stress
Molecular & Cellular Oncology, 2021 Conflicts between transcription and replication are a major source of replication stress. Our recent findings show that proper dephosphorylation of Serine 5 in the carboxy-terminal domain (CTD) of DNA-directed RNA polymerase II subunit RPB1 is needed to ...
Helga B. Landsverk +3 more
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Structure and Function of a Mycobacterial NHEJ DNA Repair Polymerase [PDF]
, 2006 Non homologous end-joining (NHEJ)-mediated repair of DNA double-strand breaks in prokaryotes requires Ku and a specific multidomain DNA ligase (LigD).
Aidan J. Doherty +59 more
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Amplification of 4–9-kb Human Genomic DNA Flanking a Known Site Using a Panhandle PCR Variant
BioTechniques, 1997 We present a method for the in vitro amplification of >6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted repeat of a portion of the known sequence, followed by single-primer polymerase chain ...
Douglas H. Jones, Stanley C. Winistorfer
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