Results 11 to 20 of about 249,414 (217)
PLoS ONE, 2013 Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3' terminal phosphodiester bond.
Alicia del Prado +4 more
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Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair. [PDF]
PLoS Genetics, 2009 Polymerase micro (Polmicro) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Polmicro deficiency results in impaired Vkappa-Jkappa recombination and altered somatic hypermutation and ...
Daniel Lucas +8 more
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Engineered DNA Polymerase Improves PCR Results for Plastid DNA
Applications in Plant Sciences, 2013 Premise of the study: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material.
Melanie Schori +3 more
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Optimization of Gene Design, PCR Assembly and Site-Directed Mutagenesis for the Synthesis of Beauveria Bassiana Protease Gene [PDF]
Journal of Sciences, Islamic Republic of Iran, 2021 Synthetic gene construction is one of the components of synthetic biology. It can be used for various purposes such as to optimize gene expression.
Muhammad Azharuddin Azali +4 more
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Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair.
Igor Oscorbin, Maxim Filipenko
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Mechanisms of PARP inhibitor resistance in cancer and insights into the DNA damage response
Genome Medicine, 2018 Editorial summary Inhibitors of poly(ADP-ribose) polymerase (PARPi) have entered the clinic for the treatment of patients with cancers that lack homology-directed DNA repair, but drug resistance remains a clinical hurdle.
Paola Francica, Sven Rottenberg
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New link between the RNA polymerase II-CTD and replication stress
Molecular & Cellular Oncology, 2021 Conflicts between transcription and replication are a major source of replication stress. Our recent findings show that proper dephosphorylation of Serine 5 in the carboxy-terminal domain (CTD) of DNA-directed RNA polymerase II subunit RPB1 is needed to ...
Helga B. Landsverk +3 more
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Amplification of 4–9-kb Human Genomic DNA Flanking a Known Site Using a Panhandle PCR Variant
BioTechniques, 1997 We present a method for the in vitro amplification of >6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted repeat of a portion of the known sequence, followed by single-primer polymerase chain ...
Douglas H. Jones, Stanley C. Winistorfer
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The ability to form homodimers is essential for RDM1 to function in RNA-directed DNA methylation. [PDF]
PLoS ONE, 2014 RDM1 (RNA-DIRECTED DNA METHYLATION1) is a small plant-specific protein required for RNA-directed DNA methylation (RdDM). RDM1 interacts with RNA polymerase II (Pol II), ARGONAUTE4 (AGO4), and the de novo DNA methyltransferase DOMAINS REARRANGED ...
Taku Sasaki +4 more
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Frontiers in Microbiology, 2016 Pseudorabies virus (PRV) DNA replication occurs in the nuclei of infected cells and requires the viral DNA polymerase. The PRV DNA polymerase comprises a catalytic subunit, UL30, and an accessory subunit, UL42, that confers processivity to the enzyme ...
Yi-Ping eWang +6 more
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