Results 21 to 30 of about 410,825 (308)

Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage [PDF]

, 2017
The p53 tumour suppressor regulates the transcription initiation of selected genes by binding to specific DNA sequences at their promoters. Here we report a novel role of p53 in transcription elongation in human cells.
Boros, I.M. (Imre M.), Borsos, B.N. (Barbara N.), Gyenis, A. (Ákos), Huliák, I. (Ildikó), Majoros, H. (Hajnalka), Pankotai, T. (Tibor), Pukler, P. (Peter), Ujfaludi, Z. (Zsuzsanna)   +7 more
core   +7 more sources

Dynamic regulation of ARGONAUTE4 within multiple nuclear bodies in Arabidopsis thaliana. [PDF]

PLoS Genetics, 2008
DNA methylation directed by 24-nucleotide small RNAs involves the small RNA-binding protein ARGONAUTE4 (AGO4), and it was previously shown that AGO4 localizes to nucleolus-adjacent Cajal bodies, sites of snRNP complex maturation. Here we demonstrate that
Carey F Li, Ian R Henderson, Liang Song, Nina Fedoroff, Thierry Lagrange, Steven E Jacobsen   +5 more
doaj   +1 more source

RanBP2-Mediated SUMOylation Promotes Human DNA Polymerase Lambda Nuclear Localization and DNA Repair [PDF]

, 2020
Cellular DNA is under constant attack by a wide variety of agents, both endogenous and exogenous. To counteract DNA damage, human cells have a large collection of DNA repair factors. Among them, DNA polymerase lambda (Polλ) stands out for its versatility,
Cortés Ledesma, Felipe, García Domínguez, Mario, Herrero Ruiz, Andrés M., Moreno Oñate, Marta, Ruiz Pérez, José Francisco   +4 more
core   +1 more source

Kinetic analysis of an efficient DNA-dependent TNA polymerase. [PDF]

, 2005
alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs).
Chaput, John C, Horhota, Allen, Ichida, Justin K, McLaughlin, Larry W, Szostak, Jack W, Yu, Biao, Zou, Keyong   +6 more
core   +2 more sources

Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis. [PDF]

, 2019
The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression.
Chory, Joanne, Feng, Suhua, Gallego-Bartolomé, Javier, Gardiner, Jason, Ghoshal, Basudev, Jacobsen, Steven E, Kuo, Peggy Hsuanyu, Liu, Wanlu, Park, Soo Young, Zhao, Jenny Miao-Chi   +9 more
core   +2 more sources

The Nuclear Proteins TP73 and CUL4A Confer Resistance to Cytarabine by Induction of Translesion DNA Synthesis via Mono-ubiquitination of PCNA

HemaSphere, 2022
Resistance to cytarabine is a key problem in the treatment of acute myeloid leukemia (AML). To understand the molecular biology of resistance to cytarabine, a viability-based chemosensitizer screen was utilized. We screened synthetic lethal targets using
Miriam Rehberger, Jonas A. Schäfer, Anna-Maria Krampitz, Anne Catherine Bretz, Lukas Jost, Torsten Haferlach, Thorsten Stiewe, Andreas Neubauer   +7 more
doaj   +1 more source

Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis [PDF]

, 2018
Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues.
Baner, Bloom, Cadwell, Carlson, Copp, Cozens, Cunningham, Currin, Davidson, Dennig, Dickinson, Ellefson, Ellefson, Engler, Fox, Gibson, Guzman, Hames, Hogrefe, Jeschek, Jin, Jones, Kan, Khersonsky, Leconte, Lee, Lohman, Nov, Packer, Pai, Peng, Pinheiro, Podgornaia, Pédelacq, Quaglia, R Core Team, Ralser, Reetz, Reetz, Seyfang, Shapiro, Shin, Stemmer, Sunden, Tang, Tee, Turner, Tóth-Petróczy, Wrenbeck, Wäneskog, Yang, Young, Zhang   +52 more
core   +1 more source

Dual role of φ29 DNA polymerase Lys529 in stabilisation of the DNA priming-terminus and the terminal protein-priming residue at the polymerisation site.

PLoS ONE, 2013
Resolution of the crystallographic structure of φ29 DNA polymerase binary and ternary complexes showed that residue Lys529, located at the C-terminus of the palm subdomain, establishes contacts with the 3' terminal phosphodiester bond.
Alicia del Prado, José M Lázaro, Laurentino Villar, Margarita Salas, Miguel de Vega   +4 more
doaj   +1 more source

Altered hematopoiesis in mice lacking DNA polymerase mu is due to inefficient double-strand break repair. [PDF]

PLoS Genetics, 2009
Polymerase micro (Polmicro) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Polmicro deficiency results in impaired Vkappa-Jkappa recombination and altered somatic hypermutation and ...
Daniel Lucas, Beatriz Escudero, José Manuel Ligos, Jose Carlos Segovia, Juan Camilo Estrada, Gloria Terrados, Luis Blanco, Enrique Samper, Antonio Bernad   +8 more
doaj   +1 more source

Engineered DNA Polymerase Improves PCR Results for Plastid DNA

Applications in Plant Sciences, 2013
Premise of the study: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material.
Melanie Schori, Maryke Appel, Alexarae Kitko, Allan M. Showalter   +3 more
doaj   +1 more source