Results 41 to 50 of about 881,637 (262)
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Revista Brasileira de Hematologia e Hemoterapia, 2015 Introduction: Minimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia.
Francisco Danilo Ferreira Paula +7 more
doaj +1 more source
Application of Nanomaterials to Enhance Polymerase Chain Reaction
Molecules, 2022 Polymerase Chain Reaction (PCR) is one of the most common technologies used to produce millions of copies of targeted nucleic acid in vitro and has become an indispensable technique in molecular biology.
Zhu Yang +5 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Polymerase chain reaction methods (PCR) in agrobiotechnology [PDF]
Selekcija i Semenarstvo, 2006 The agricultural biotechnology applies polymerase chain reaction (PCR) technology at numerous steps throughout product development. The major uses of PCR technology during product development include gene discovery and cloning, vector construction ...
Taški-Ajduković Ksenija +3 more
doaj
Efficiency of the polymerase chain reaction
Chemical Engineering Science, 2010 The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology such as gene expression studies, mutation detection, forensic analysis and pathogen detection. Increasingly quantitative real time PCR is used to assess copy numbers from overall yield.
Booth, Christine S. +5 more
openaire +3 more sources
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
A portable low-cost polymerase chain reaction device
HardwareXPolymerase chain reaction (PCR) is a cornerstone technique in molecular biology and clinical diagnostics. However, conventional PCR systems are often bulky and prohibitively expensive, limiting their use in resource-limited settings.
Kan Luo +6 more
doaj +1 more source
Case Reports in Infectious Diseases, 2015 This report presents a case of tuberculous lymphadenitis that was difficult to diagnose using polymerase chain reaction analysis. An 80-year-old Japanese female was hospitalized due to swollen cervical lymph nodes.
Hirokazu Taniguchi +9 more
doaj +1 more source
Brazilian Journal of Infectious Diseases, 2013 To test the validity of the polymerase chain reaction for diagnosing American visceral leishmaniasis, 88 suspected cases were studied. Diagnosis was confirmed in 47 (53.5%) and ruled out in 41 (46.5%) patients. Samples of bone marrow and peripheral blood
Celia Maria Silva Pedrosa +3 more
doaj +1 more source