Results 51 to 60 of about 218,617 (259)

Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia [PDF]

, 2020
To diagnose malaria, microscopic examination is still considered as the standard tool, but still there are certain reservations. This study aimed to compare the identification of Plasmodium species by microscopy and polymerase chain reaction (PCR).
Trieva Verawaty Butarbutar, -, Puspa Wardhani, -, Christophorus Oetama Adiatmaja, -, Amarensi Milka Betaubun, -, Aryati, -   +4 more
core   +1 more source

Application of Nanomaterials to Enhance Polymerase Chain Reaction

Molecules, 2022
Polymerase Chain Reaction (PCR) is one of the most common technologies used to produce millions of copies of targeted nucleic acid in vitro and has become an indispensable technique in molecular biology.
Zhu Yang, Bei Shen, Lihuan Yue, Yuqing Miao, Yihong Hu, Ruizhuo Ouyang   +5 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Application of polymerase chain reaction for detection of Legionella pneumophila in serum samples

, 1998
ObjectiveTo apply the polymerase chain reaction (PCR) to serum samples for the rapid diagnosis of Legionnaire's disease using the L5SL9 and L5SR93 primers designed to generate a 104-base-pair (bp) fragment from the 5S RNA gene of Legionella spp.
Zorbas, I, Alexiou-Daniel, S, Papa, A, Zorbas, I., Papoutsi, A, Papa, A., Papoutsi, A., Antoniadis, A., Antoniadis, A, Stylianakis, A, Alexiou-Daniel, S., Stylianakis, A., Lambropoulos, A.F., Lambropoulos, A   +13 more
core   +1 more source

Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia

Revista Brasileira de Hematologia e Hemoterapia, 2015
Introduction: Minimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia.
Francisco Danilo Ferreira Paula, Silvana Maria Elói-Santos, Sandra Guerra Xavier, Mônica Aparecida Ganazza, Patricia Yoshioka Jotta, José Andrés Yunes, Marcos Borato Viana, Juliana Godoy Assumpção   +7 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Detection of bancroftian filariasis in human blood samples from Sorsogon province, the Philippines by polymerase chain reaction.

, 2001
The polymerase chain reaction (PCR) was used for diagnosing Wuchereria bancrofti infection in a small village in the province of Sorsogon, the Philippines.
Pasay, MC, Ramirez, BL, Salazar, F, Santiago, ML, Torres, EP, Hafalla, JC, Alamares, JG   +6 more
core   +1 more source

Real-time Polymerase Chain Reaction [PDF]

, 2020
: The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule.
Mei-Ying Chuang, Geroge Chen, Kuan-Jiunn Shieh, X Tracy Qiao, Hongbao Ma   +4 more
core  

Polymerase Chain Reaction detection of deformed wing virus (DWV) in Apis mellifera and Varroa destructor [PDF]

, 2004
We have developed a specific assay for the detection of deformed wing virus (DWV) in Apis mellifera L. and Varroa destructor based on the reverse transcriptase polymerase chain reaction (RT-PCR) technology.
Gauthier, L., Cousserans, Francois, Brenda V. Ball, Gauthier, Laurent, Colin, Marc Edouard, Jouve, Sandrine, Colin, Marc, Laurent Gauthier, Bergoin, Max, Bergoin, M., Ball, B. V., François Cousserans, Colin, M. E., Ball, Brenda V., Tentcheva, D., Diana Tentcheva, Canabady-Rochelle, L., Marc Edouard Colin, Max Bergoin, Dainat, B., Benjamin Dainat, Sandrine Jouve, Cousserans, F., Jouve, S., Tentcheva, Diana, Laetitia Canabady-Rochelle, Canabady-Rochelle, Laetitia, Ball, Brenda, Dainat, Benjamin   +28 more
core   +1 more source