Results 51 to 60 of about 110,528 (265)
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Multiaxial Fatigue Properties of 2A12 Aluminum Alloy Under Different Stress Amplitude Ratio Loadings
The multiaxial fatigue behavior of 2A12 aluminum alloy was studied with SDN100/1000 electro-hydraulic servo tension-torsion fatigue tester under different stress amplitude ratios, the fracture morphology and the fatigue loading curve were observed to ...
CHEN Ya-jun +4 more
doaj +1 more source
Amplitude ratio tomography for regional phase Q [PDF]
We image regional seismic phase attenuation (Q) by extending amplitude ratio techniques, which are widely used to determine average Q within a sampled region, to obtain laterally varying Q structure using tomographic techniques. The method assumes that source radiation is isotropic and that the amplitude ratios can be modeled by a two‐dimensional Q map
W. S. Phillips +2 more
openaire +1 more source
Age effects on frequency amplitude ratio of cVEMP
Abstract Background The vestibular system can perceive sound at high intensity levels. The recording of vestibular evoked myogenic potentials (VEMPs) is one of the clinical techniques that capture the vestibular system when activated by sound.
Abeir Osman Dabbous +3 more
openaire +2 more sources
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel +4 more
wiley +1 more source
ABSTRACT Objective In multiple sclerosis, the optimal time for deploying a therapeutic intervention is before the central nervous system is damaged; given the success of trials treating the earliest stage of MS, the radiologically isolated syndrome, developing primary prevention strategies is an important next challenge.
Amy W. Laitinen +7 more
wiley +1 more source
Regional waveforms of deep‐focus Tonga‐Fiji earthquakes indicate anomalous traveltime differences (ScS2‐ScS) and amplitude ratios (ScS2/ScS) of the phases ScS and ScS2.
Carlos A. M. Chaves, Jeroen Ritsema
doaj +1 more source
Background: The hip abductors (HAB), quadriceps (Q) and hamstrings (H) reciprocal strength ratios are predictors of electromyography (EMG) amplitude during load carrying walking at moderate intensity.
Petr Stastny +6 more
doaj +1 more source

