Results 31 to 40 of about 1,748 (246)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Ziziphus spina-christi Leaf-Derived Carbon Dots as a Fluorescence Nanosensor to Evaluate Rifaximin Antibacterial via Inner Filter Effect: Greenness and Whiteness Studies

Chemosensors, 2023
Rifaximin (RFX) is a non-absorbable antibiotic with broad-spectrum efficacy. It treats travelers’ diarrhea, irritable bowel syndrome, non-systematic bacterial diarrhea, bowel infections, overgrowth syndrome, and enteric infections.
Mohamed A. El Hamd, Marzough Aziz Albalawi, Hassanien Gomaa, Bassam Shaaban Mohammad, Rady F. Abdul-Kareem, Reem H. Obaydo, Wejdan T. Alsaggaf, Safaa F. Saleh, Manal A. Alossaimi, Mohamed A. Abdel-Lateef   +9 more
doaj   +1 more source

Hasse diagram as a green analytical metrics tool: ranking of methods for benzo[a]pyrene determination in sediments [PDF]

Analytical and Bioanalytical Chemistry, 2016
This study presents an application of the Hasse diagram technique (HDT) as the assessment tool to select the most appropriate analytical procedures according to their greenness or the best analytical performance. The dataset consists of analytical procedures for benzo[a]pyrene determination in sediment samples, which were described by 11 variables ...
Bigus, Paulina, Tsakovski, Stefan, Simeonov, Vasil, Namieśnik, Jacek, Tobiszewski, Marek   +4 more
openaire   +2 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Assessment of the Greenness of HPLC Methods Employing Common Detectors for Analysis of Caffeine in Pharmaceutical Dosage Forms

Journal of Chemistry
Caffeine, the world’s most widespread central nervous system stimulant, naturally exists in various plants species. Caffeine impacts several beneficial physiological functions; accordingly, caffeine is widely consumed from both herbal and pharmaceutical ...
Mohammed Alqarni, Ibrahim A. Naguib, Hassan Arida, Nawaf Abdullah Althagafi, Mahir Saleh Aboras, Khalid Amer Alshehri, Ahmed Saeed Alzahrani, Mohammed Salem Alsuwat, Heba-Alla H. Abd-ElSalam   +8 more
doaj   +1 more source

A green and robust LC–MS/MS bioanalytical method for sulopenem etzadroxil and probenecid: Optimization, validation, and pharmacokinetic application

Journal of Pharmaceutical and Biomedical Analysis Open
A novel, green, and robust Liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous quantification of sulopenem etzadroxil and probenecid in rat plasma, with application to pharmacokinetic studies.
Niloufer Tasnim Khazi, Kumaraswamy Gandla, Lalitha Repudi   +2 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Development and validation of a novel RP-HPLC method for determination of Remdesivir: Investigation of the greenness for the proposed method

Results in Chemistry
Remdesivir, a nucleotide prodrug of an adenosine analogue, exhibits antiviral activity against SARS-CoV-2 by binding to the viral RNA-dependent polymerase and disrupting viral replication.
Karthika Paul, B.H. Jaswanth Gowda, R.S. Chandan   +2 more
doaj   +1 more source