Results 21 to 30 of about 21,890 (235)

Nitrogen Oxyanion-dependent Dissociation of a Two-component Complex That Regulates Bacterial Nitrate Assimilation [PDF]

, 2013
Nitrogen is an essential nutrient for growth and is readily available to microbes in many environments in the form of ammonium and nitrate. Both ions are of environmental significance due to sustained use of inorganic fertilizers on agricultural soils ...
Goldman, Lin, Merrick, Morth, Ogawa, Wilson, Wu   +6 more
core   +1 more source

Binding of the kringle‐2 domain of human plasminogen to streptococcal PAM‐type M‐protein causes dissociation of PAM dimers

MicrobiologyOpen, 2021
The direct binding of human plasminogen (hPg), via its kringle‐2 domain (K2hPg), to streptococcal M‐protein (PAM), largely contributes to the pathogenesis of Pattern D Group A Streptococcus pyogenes (GAS).
Olawole Ayinuola, Yetunde A. Ayinuola, Cunjia Qiu, Shaun W. Lee, Victoria A. Ploplis, Francis J. Castellino   +5 more
doaj   +1 more source

Molar mass and solution conformation of branched alpha(1 - 4), alpha(1 - 6) Glucans. Part I: Glycogens in water [PDF]

, 2008
Solution molar masses and conformations of glycogens from different sources (rabbit, oyster, mussel and bovine) were analysed using sedimentation velocity in the analytical ultracentrifuge, size-exclusion chromatography coupled to multi-angle laser light
Ang, S., Harding, S. E., Hill, S. E., Lewis, S., Morris, Gordon, Nobbmann, U., Schafer, B.   +6 more
core   +1 more source

Crystallization of Adenylylsulfate Reductase from Desulfovibrio gigas: A Strategy Based on Controlled Protein Oligomerization [PDF]

, 2011
Adenylylsulfate reductase (adenosine 5′-phosphosulfate reductase, APS reductase or APSR, E.C.1.8.99.2) catalyzes the conversion of APS to sulfite in dissimilatory sulfate reduction. APSR was isolated and purified directly from massive anaerobically grown
Chan, Sunney I., Chen, Chun-Jung, Chiang, Yuan-Lan, Chuankhayan, Phimonphan, Fang, Jou-Yin, Hsieh, Yin-Cheng, Huang, Yen-Chieh, Liu, Ming-Yih, Wang, Vincent C.-C., Yang, Ming-Chi   +9 more
core   +1 more source

Molecular flexibility of citrus pectins by combined sedimentation and viscosity analysis [PDF]

, 2007
The flexibility/rigidity of pectins plays an important part in their structure-function relationship and therefore on their commercial applications in the food and biomedical industries.
Alan Smith, Alvaro Ortega, Axelos, Bohdanecky, Braccini, Chapman, Corredig, Cros, Fishman, Gordon A. Morris, Harding, Harding, Harding, Harding, Hearst, Hearst, Huggins, Jonathan Castile, José García de al Torre, Kraemer, Kratky, Lapasin, Morris, Morris, Morris, Norziah, Noto, Pavlov, Pavlov, Perez, Picout, Pilgrim, Ralet, Ralston, Ridley, Rowe, Schuck, Schuck, Stephen E. Harding, Stockmayer, Tanford, Tombs, Van Buren, Wales, Willats, Wyatt, Yamakawa, Yoo   +47 more
core   +1 more source

A 6 bp Z-DNA hairpin binds two Zα domains from the human RNA editing enzyme ADAR1 [PDF]

, 1999
The Zα domain of the human RNA editing enzyme double-stranded RNA deaminase I (ADAR1) binds to left-handed Z-DNA with high affinity. We found by analytical ultracentrifugation and CD spectroscopy that two Zα domains bind to one d(CG)3T4(CG)3 hairpin ...
Behlke, Joachim, Herbert, Alan, Lowenhaupt, Ky, Oschkinat, Hartmut, Rich, Alexander, Schade, Markus   +5 more
core   +1 more source

Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

eLife, 2016
The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of assemblies requires accurate measurement of their stoichiometry, affinity and cooperativity, and frequently consideration of ...
Huaying Zhao, Yan Fu, Carla Glasser, Eric J Andrade Alba, Mark L Mayer, George Patterson, Peter Schuck   +6 more
doaj   +1 more source

The Solution Structures of Two Human IgG1 Antibodies Show Conformational Stability and Accommodate Their C1q and FcγR Ligands. [PDF]

, 2015
The human IgG1 antibody subclass shows distinct properties compared with the IgG2, IgG3, and IgG4 subclasses and is the most exploited subclass in therapeutic antibodies. It is the most abundant subclass, has a half-life as long as that of IgG2 and IgG4,
Abe, Andrusier, Ashish, Ashton, Boehm, Brekke, Bruhns, Brüggemann, Calmettes, Collins, Dall'Acqua, Dangl, Davies, Deisenhofer, Diebolder, Dorai, Feinstein, Furtado, Gaboriaud, Gar Kay Hui, Garcia de la Torre, García De La Torre, Glatter, Gregory, Guddat, Guex, Hamilton, Harris, Harris, Heenan, Hezareh, Hughes-Jones, Idusogie, Isenman, Iwura, Jayesh Gor, Jefferis, Kilár, Klein, Laue, Lilyestrom, Lucy E. Rayner, Lund, Luo, Manning, Marquart, Michaelsen, Narayanan, Paul A. Dalby, Perkins, Perkins, Perkins, Perkins, Phillips, Pilz, Radaev, Radaev, Rayner, Rayner, Remmele, Richard K. Heenan, Richardson, Roux, Saphire, Saphire, Sarma, Schneider, Schneidman-Duhovny, Schuck, Schuck, Semenyuk, Sondermann, Stephen J. Perkins, Strohl, Sun, Tamm, Tao, Thommesen, Wang, Yang   +79 more
core   +1 more source

Biophysical evidence for intrinsic disorder in the C-terminal tails of the epidermal growth factor receptor (EGFR) and HER3 receptor tyrosine kinases [PDF]

, 2016
The epidermal growth factor receptor (EGFR)/ErbB family of receptor tyrosine kinases includes oncogenes important in the progression of breast and other cancers, and they are targets for many drug development strategies.
Bose, Ron, Keppel, Theodore R, Monsey, John, Murray, Elisa M, Sarpong, Kwabena, Zhu, Jian   +5 more
core   +2 more sources

Determination of the two-dimensional distributions of gold nanorods by multiwavelength analytical ultracentrifugation

Nature Communications, 2018
Characterization of nanoparticles is a complex and important problem for the vast number of applications that require them. Here, the authors present a method to uncover the two-dimensional distribution of length and diameter of anisotropic nanoparticles
Simon E. Wawra, Lukas Pflug, Thaseem Thajudeen, Carola Kryschi, Michael Stingl, Wolfgang Peukert   +5 more
doaj   +1 more source