Results 41 to 50 of about 81,279 (338)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Granular discharge and clogging for tilted hoppers

open access: yes, 2009
We measure the flux of spherical glass beads through a hole as a systematic function of both tilt angle and hole diameter, for two different size beads.
C. Mankoc   +29 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Cross-sectional river shapes: A variational discharge-resistance formulation [PDF]

open access: yes, 1999
Cross-sectional river shapes were obtained from a variational principle: minimizing the bed friction for a given discharge and a given maximum lateral bed slope (angle of repose).
Beckum, F.P.H. van   +2 more
core   +1 more source

Dynamics of axial separation in long rotating drums

open access: yes, 1998
We propose a continuum description for the axial separation of granular materials in a long rotating drum. The model, operating with two local variables, concentration difference and the dynamic angle of repose, describes both initial transient traveling
E. Clément   +16 more
core   +1 more source

Network Localization of Fatigue in Multiple Sclerosis

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Fatigue is among the most common symptoms and one of the main factors determining the quality of life in multiple sclerosis (MS). However, the neurobiological mechanisms underlying fatigue are not fully understood. Here we studied lesion locations and their connections in individuals with MS, aiming to identify brain networks ...
Olli Likitalo   +12 more
wiley   +1 more source

Parameter Calibration of Discrete Element Model of Wine Lees Particles

open access: yesApplied Sciences
In order to investigate the contact characteristics of the mechanical parts of the brewing robot with wine lees particles, it is essential to calibrate the parameters of the discrete elemental model of wine lees particles.
Xiaoyuan Zhang   +4 more
doaj   +1 more source

Determination of Material and Interaction Properties of Granular Fertilizer Particles Using DEM Simulation and Bench Testing

open access: yesAgriculture, 2023
The discrete element method (DEM) is an effective tool for obtaining qualitative and quantitative information on particle motion, which aids in the design and optimization of agricultural equipment structures.
Xiaolong Lei   +8 more
doaj   +1 more source

The Origin of a Repose Angle: Kinetics of Rearrangements for Granular Materials

open access: yes, 2001
A microstructural theory of dense granular materials is presented, based on two main ideas. Firstly, that macroscopic shear results form activated local rearrangements at a mesoscopic scale. Secondly, that the update frequency of microscopic processes is
Anaël Lemaître   +8 more
core   +1 more source

Reduced Muscular Carnosine in Proximal Myotonic Myopathy—A Pilot 1H‐MRS Study

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Myotonic dystrophy type 2 (proximal myotonic myopathy, PROMM) is a progressive multisystem disorder with muscular symptoms (proximal weakness, pain, myotonia) and systemic manifestations such as diabetes mellitus, cataracts, and cardiac arrhythmias.
Alexander Gussew   +11 more
wiley   +1 more source

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