Studies on the Fluorescence of 1‐Anilino‐8‐naphthalenesulfonate by the Membranes of the Sarcoplasmic Reticulum [PDF]
The quantum yield of 1‐anilino‐8‐naphthalenesulfonate associated with native and lipid‐depleted sarcoplasmic vesicles is nearly identical. The slight increase of the average apparent dissociation constant of the interaction of this dye with lipid‐depleted vesicles is accompanied by a decrease in the number of binding sites for the dye.
Augustin, J., Hasselbach, W.
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Biodegradation of 8-anilino-1-naphthalenesulfonic acid by Pseudomonas aeruginosa
Pseudomonas aeruginosa, isolated from soil near tannery effluent was able to degrade 8-anilino-1-naphthalenesulfonic acid (ANSA), a sulfonated aromatic amine. The organism degraded this amine up to a concentration of 1,200 mg l(-1) using glucose and ammonium nitrate as carbon and nitrogen sources respectively.
C, Valli Nachiyar, G, Suseela Rajakumar
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Membrane-surfactant Interactions in Lipid Micelles Labeled with l-Anilino-8-naphthalenesulfonate [PDF]
Sonicated lipid micelles, formed from phospholipids isolated from yolks of fresh hen eggs, were used as a model membrane system for studying the effects of several surfactants on membrane properties. The interactions of the surfactants with the model system were followed through the fluorescence of the hydrophobic probe l-anilino-8-naphthalenesulfonate.
G M, Miller, J B, John
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Tryptophan and 8-anilino-1-naphthalenesulfonate compete for binding to trp repressor
Anilinonaphthalenesulfonate (ANS) and tryptophan compete for binding to the trp repressor protein; thus, the fluorescence decrease associated with ANS dissociation can be used as a fluorometric marker for tryptophan binding to the protein. Using this approach, the tryptophan equilibrium dissociation constant was measured at 25 degrees C to be 3.7 ...
W Y, Chou, C, Bieber, K S, Matthews
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Effects of Anions on the Fluorescence Emission of the 1-Anilino-8-naphthalenesulfonate--Phosphoglycerate Kinase Complex. [PDF]
When 1-anilino-8-naphthalenesulfonate (ANS) interacts with phosphoglycerate kinase (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) its fluorescence is enhanced and a blue shift occurs. There is evidence that ANS binds to the site of the nucleotide substrate.
Muhammed M. Khamis +8 more
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The binding of 1-anilino-8-naphthalenesulfonate (ANS) to dipalmitoyl-phosphatidycholine (DPPC)-sonicated vesicles was measured by a fluorimetric method in the vicinity of the gel-to-liquid crystalline phase transition temperature (Tm). A similar measurement was performed on large multimellar DPPC vesicles through equilibrium dialysis. Both measurements
Jacobson, K., Papahadjopoulos, D.
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An Improved Protocol for Better Detection of Protein Using 8-Anilino-1-naphthalenesulfonate
This study describes the optimum conditions at the staining time and the signal intensity for using 8-anilino-1-naphthalenesulfonate (ANS) as a fluorescent probe to detect proteins in SDS-PAGE. Using the optimized protocol, protein can be easily detected by short time fixing (20 min) and washing (2 × 5 min), followed by 10 min of staining.
MA, Weide +5 more
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Substrate binding to phosphoglycerate kinase monitored by 1-anilino-8-naphthalenesulfonate.
The interaction between 1-anilino-8-naphthalenesulfonate (ANS) and yeast phosphoglycerate kinase (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) and the use of ANS as a probe for studying the structure and function of phosphoglycerate kinase has been investigated.
E, Wiksell, M, Larsson-Raźnikiewicz
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Revisiting the Rate-Limiting Step of the ANS-Protein Binding at the Protein Surface and Inside the Hydrophobic Cavity. [PDF]
Ota C, Tanaka SI, Takano K.
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Bioactive Properties of a Novel Antibacterial Dye Obtained from Laccase-Mediated Oxidation of 8-Anilino-1-naphthalenesulfonic Acid. [PDF]
Polak J +6 more
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