Results 221 to 230 of about 377,815 (340)

Indoleamine 2,3‐dioxygenase 1 inhibition reverses cancer‐associated fibroblast‐mediated immunosuppression in high‐grade serous ovarian cancer

FEBS Open Bio, EarlyView.
CAF‐mediated immunosuppression in ovarian cancer is driven by IDO1, reducing T‐cell function. Inhibiting IDO1 restores T‐cell proliferation and cytotoxicity, increases cancer cell apoptosis, and may help overcome CAF‐induced immune suppression in high‐grade serous ovarian cancer. Targeting IDO1 may improve antitumor immunity.
Hyewon Lee, Jung Yoon Ho, In Sun Hwang, Youn Jin Choi   +3 more
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Declaration of transparency and scientific rigour: checklist for animal experimentation. [PDF]

Br J Pharmacol, 2018
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

The animal experimentation quandary: stuck between legislation and scientific freedom: More research and engagement by scientists is needed to help to improve animal welfare without hampering biomedical research. [PDF]

EMBO Rep, 2016
Bert B, Chmielewska J, Hensel A, Grune B, Schönfelder G.   +4 more
europepmc   +1 more source

From Harm-Benefit Analysis to Phase-out Planning for Animal Experimentation

Nico Dario Müller, M Balls, K Bayertz, K Bayne, S Degreeve, Bk (schweizerische, ) Bundeskanzlei, Bk (schweizerische, ) Bundeskanzlei, Bk (schweizerische, ) Bundeskanzlei, Bk (schweizerische, ) Bundeskanzlei, Schweizerische Bk (, ) Bundeskanzlei, C Decelle, T Everitt, J Guillen, J Laber, K, B Brophy, S Fortunato, C Bergstrom, K Brner, J Evans, D Helbing, S Milojevi, A Petersen, F Radicchi, R Sinatra, B Uzzi, A Vespignani, L Waltman, D Wang, A.-L Barabsi, J Foster, A Rzhetsky, J Evans, D Grimm, D Grimm, I Hutchinson, C Owen, J Bailey, L Marshall, H Constantino, T Seidle, T Mcconnell, N Mller, W Neuhaus, B Reininger-Gutmann, B Rinner, R Plasenzotti, D Wilflingseder, J De Kock, T Vanhaecke, V Rogiers, D Jrov, K Kejlov, L Knudsen, R Nielsen, B Kleuser, V Kral, C Thne-Reineke, T Hartung, G Pallocca, M Leist, S Hippenstiel, A Lang, H Spielmann, K Taylor, L Alvarez, B Williams, Nico Mller, Korrespondenzadresse Dr, D Nico, Universitt Mller, Basel   +76 more
openalex   +1 more source

Ethical Issues Associated with the Use of Animal Experimentation in Behavioral Neuroscience Research

, 2014
Frauke Ohl, F.L.B. Meijboom
openalex   +2 more sources

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source