Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
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Retraction: Oncolytic virotherapy promotes radiosensitivity in soft tissue sarcoma by suppressing anti-apoptotic MCL1 expression. [PDF]
PLOS One Editors.
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Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Systematic Review of Mammalian Models for Experimental Sporotrichosis: Pathogenesis, Methodological Variables, and Ethical Considerations. [PDF]
Corrêa-Moreira D +4 more
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In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
Inside-Out Mechanism of Referred Visceral Pain: Protocol for a Systematic Review of Cutaneous Neurogenic Inflammation in Preclinical Animal Models of Visceral Diseases. [PDF]
Yang E +6 more
europepmc +1 more source
An ELISA-Based Alternative to Mouse Bioassays for Quantitative Evaluation of Tetanus Toxin. [PDF]
Shitada C +4 more
europepmc +1 more source
Smooth Muscle Cell Specific Activity of SGK-1 Alters Aortic Stiffness and Abdominal Aortic Aneurysm Growth. [PDF]
Anderson M +9 more
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