Results 231 to 240 of about 643,393 (307)

Motion-Style Scalp Acupuncture Ameliorates Post-Stroke Muscle Spasticity Through Cerebral Blood Flow Augmentation and 5-HT_2AR-Mediated Spinal KCC2 Reactivation. [PDF]

Brain Behav
Zhang QY, Wang JX, Ma LX, Zhong JS, Li F, Qian X, Ma LH, Xiu JY, Wang XY.   +8 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Perinatal Western-style diet exposure associated with altered sensory functional connectivity in infant Japanese macaques. [PDF]

Physiol Rep
Papadakis S, Feczko E, Ramirez JSB, Miranda-Dominguez O, Sturgeon D, Madison TJ, Perrone AJ, Earl E, Mitchell AJ, Dunn GA, Sullivan EL, Fair DA.   +11 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Simulated microgravity accurately models long-duration spaceflight effects on bone and skeletal muscle in skeletally immature mice. [PDF]

Bone Rep
Friedman MA, Zeineddine Y, Tuyambaze O, Elhawabri W, Al Shammary A, Stodieck L, Ferguson VL, Donahue HJ.   +7 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Improvement to East African style experimental huts allows for more effective evaluation of vector control products for protection against vector-borne diseases. [PDF]

Parasit Vectors
Portwood NM, Sumari G, Matowo J, Azizi S, Manunda B, Ezekia K, Mosha FW, Messenger LA, Rowland MW, Mbewe NJ.   +9 more
europepmc   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

ASQ-PHI: An adversarial synthetic data benchmark for clinical de-identification and search utility. [PDF]

Data Brief
Weatherhead J, Golovko G, McCaffrey P.
europepmc   +1 more source