Results 131 to 140 of about 4,831,623 (248)

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Advice for bad toxicologists

NAM Journal
Richard R. Rabbit, aka Thomas Hartung
doaj   +1 more source

Nonantibiotic Alternatives for Mastitis in Small Ruminants: A Scoping Review of Evidence, Gaps, and Translational Challenges. [PDF]

Vet Med Int
Odetokun IA, Famakinde DO, Ade-Yusuf RO, Basiru A, Adetona MA, Cole J, Elsabagh M, Garcia-Fruitós E, Arís A, Eltholth M.   +9 more
europepmc   +1 more source

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Preventing regrettable substitutions: a proposal for a tiered testing approach for BPA alternatives under the PARC initiative. [PDF]

Front Toxicol
Aiello-Holden K, Bartmann K, Buratti FM, Cabaton NJ, Coppola L, Corsini E, De la Vieja A, Dirven H, Dusinska M, El Yamani N, Franko N, Gostner JM, Grova N, Gütter L, Iglesias-Hernández P, Iulini M, Kodila A, Kohl Y, Lori G, Louro H, Marcon F, Mhaouty-Kodja S, Mposhi A, Naulé L, Palleschi S, Rossi B, Rundén-Pran E, Salani F, Santori N, Silva MJ, Dolenc MS, Testai E, Torres-Ruiz M, Turner J, Viguié C, Zalko D, Tait S.   +36 more
europepmc   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Comparison of human skin irritation and photo-irritation patch test data with cellular in vitro assays and animal in vivo data

, 2008
Replacement of the rabbit Draize skin irritation test or the animal photo-irritation test is in course in Europeunder the REACH chemical strategy and the Cosmetics Directive. Various in vitro protocols, including3D skin models, have been assessed.
Jirova,D., Bendova,H., Kejlova,K., Liebsch,M., Basketter,D., Spiller,E., Marriott,M., Kandarova,H.   +7 more
core  

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Development and evaluation of a robust, common protocols for reconstructed human skin models: In vitro skin irritation/corrosion testing

, 2006
Due to the increased need for information on the safety of chemicals (driven mainly by new the chemicals policy of the European Commission (REACH)) a lot of attention is paid to development and validation of in vitro methods replacing or reducing the use
Liebsch,M., Spielmann,H., Kandarova,H.
core