Results 161 to 170 of about 57,035 (299)

Path dependency and the rescuing of the biomedical research enterprise. [PDF]

Front Med Technol
Nahle Z.
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

The Role that Biobanks Can Play in Driving Animal-Free Biomedical Research. [PDF]

Expert Rev Mol Med
Speirs V, Walker A.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

NAMazing: Déjà Vu at the lab bench - Why animal-free science is the new automobile

NAM Journal
Thomas Hartung
doaj   +1 more source

Open-Source Tools for Neuromuscular Electrical Stimulation in Mouse Models: A Methodological Validation Study. [PDF]

Muscles
Odeh BH, Wellman AL, Ameye M, Atwood Z, Gray L, Saravanan A, Poluru H, Begam M, Jones TI, Roche R, Roche JA.   +10 more
europepmc   +1 more source

No alternative to animal tests for behaviour [PDF]

Nature, 2002
openaire   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Exploring ethical, sustainable and effective foetal bovine serum alternatives for <i>in vitro</i> mammalian cell culture. [PDF]

Front Toxicol
Meng H, Day PJR.
europepmc   +1 more source