Results 231 to 240 of about 3,171,897 (344)

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

open access: yesFEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques   +5 more
wiley   +1 more source

Metabolic Adaptations in Higher Animals

open access: hybrid, 1958
R.A. Freedland, A.E. Harper
openalex   +1 more source

CD44v, S1PR1, HER3, MET and cancer‐associated amino acid transporters are promising targets for the pancreatic cancers characterized using mAb

open access: yesFEBS Open Bio, EarlyView.
Novel mAbs could detect cancer‐associated membrane proteins on various type of pancreatic ductal adenocarcinomas (PDAC) by flow cytometry with viable PDAC cells and by immunohistochemistry with PDAC tissues. Results of protein expression were substantiated by mRNA expression by The Cancer Genome Atlas.
Takashi Nakano   +16 more
wiley   +1 more source

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