Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Dirt Floors and Domestic Animals Are Associated with Soilborne Exposure to Antimicrobial-Resistant <i>E. coli</i> in Rural Bangladeshi Households. [PDF]
Environ Sci TechnolErcumen A +16 more
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Serological profile of infectious bovine rhinotracheitis, bovine viral diarrhea, leptospirosis, toxoplasmosis, and neosporosis in beef cattle herds in Tocantins, Northern Brazil. [PDF]
Vet Res CommunRibeiro-Júnior JC +10 more
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Selenium Content of the Gonads of the Domestic Dog (<i>Canis lupus f. familiaris</i>) in Relation to Sex, Age, and Reproductive Disorders. [PDF]
Animals (Basel)Skibniewska E +4 more
europepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
FEBS Open Bio, EarlyView.Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
wiley +1 more source
Early-life animal and farming exposures and the risk of rheumatoid arthritis: findings from the E3N French cohort study. [PDF]
RMD OpenDusser P +9 more
europepmc +1 more source