Results 91 to 100 of about 7,021,714 (299)

High affinity antigen recognition of the dual specific variants of herceptin is entropy-driven in spite of structural plasticity.

PLoS ONE, 2011
The antigen-binding site of Herceptin, an anti-human Epidermal Growth Factor Receptor 2 (HER2) antibody, was engineered to add a second specificity toward Vascular Endothelial Growth Factor (VEGF) to create a high affinity two-in-one antibody bH1 ...
Jenny Bostrom, Lauric Haber, Patrick Koenig, Robert F Kelley, Germaine Fuh   +4 more
doaj   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

De novo design of epitope-specific antibodies via a structure-driven computational workflow

Nature Communications
Accurate modeling of antibody-antigen complex structures holds significant potential for advancing biomedical research and the design of therapeutic antibodies.
Fandi Wu, Yu Zhao, JiaXiang Wu, Biaobin Jiang, Bing He, Longkai Huang, Chenchen Qin, Yang Xiao, Fan Yang, Rubo Wang, Ningqiao Huang, Huaxian Jia, Yuyi Liu, Houtim Lai, Tingyang Xu, Fang Wang, Zihan Wu, Yidong Song, Shaoning Li, Wei Liu, Yu Rong, Peilin Zhao, Jianhua Yao   +22 more
doaj   +1 more source

Complexes of allergen and specific IgG antibodies activate human neutrophils

Frontiers in Allergy
AimTo investigate whether AICs activate neutrophils via FcγR and influence allergen-uptake and presentationMethodsMajor birch pollen allergen was incubated with different monoclonal antibodies (mAbs) and their effector-attenuated LALA-variants.
Bibiana Aigner, Gordana Wozniak-Knopp, Nahla Ibrahim, Ute Vollmann, Christine Brostjan, Barbara Bohle   +5 more
doaj   +1 more source

Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen

Journal of Nanobiotechnology, 2016
Background Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP.
Na-Young Ha, Hyun Mu Shin, Prashant Sharma, Hyun Ah Cho, Chan-Ki Min, Hong-il Kim, Nguyen Thi Hai Yen, Jae-Seung Kang, Ik-Sang Kim, Myung-Sik Choi, Young Keun Kim, Nam-Hyuk Cho   +11 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Antigen-antibody interaction

, 2007
The clonal selection theory of acquired immunity provides a relatively graceful to comprehend explanation for the diversity and specificity of the acquired immune system.
Jason Brownlee (6868694)
core   +1 more source

Sequence-based engineering of pH-sensitive antibodies for tumor targeting or endosomal recycling applications

mAbs
The engineering of pH-sensitive therapeutic antibodies, particularly for improving effectiveness and specificity in acidic solid-tumor microenvironments, has recently gained traction.
Wanlei Wei, Traian Sulea
doaj   +1 more source

TEKNIK IMUNOHISTOKIMIA SEBAGAI PENDETEKSI ANTIGEN SPESIFIK PENYAKIT INFEKSI

Journal of Dentistry Indonesia, 2015
Infections may be caused by bacteria, viruses, fungi, and parasites. The diagnosis can be established on the basis of signs and symptoms, should be representative of the disease process, and should be obtainal before drug administration. Diagnosis may be
Yani Rahayu, Elza Auerkari
doaj   +1 more source