Results 301 to 310 of about 1,703,096 (348)
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Oral delivery of siRNA and antisense oligonucleotides
Journal of Drug Targeting, 2009Inhibition of gene expression with antisense oligonucleotides or RNA interference (RNAi) mediated gene silencing by small interfering RNA (siRNA) has tremendous potential to silence the expression of disease-causing genes in the clinic. A major hurdle to their widespread clinical use is the safe and efficient delivery to target cells in vivo.
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PAMAM Dendrimers as Delivery Agents for Antisense Oligonucleotides
Pharmaceutical Research, 1999To investigate the potential use of PAMAM dendrimers for the delivery of antisense oligonucleotides into cells under conditions that mimic the in vivo environment.We used HeLa cells stably transfected with plasmid pLuc/705 which has a luciferase gene interrupted by a human beta-globin intron mutated at nucleotide 705, thus causing incorrect splicing ...
H, Yoo, P, Sazani, R L, Juliano
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Delivery of Antisense Oligonucleotides to the Vascular Wall
2003Antisense oligonucleotides are short segments of synthetic DNA designed to contain sequences of bases complementary to the DNA or RNA of a particular target gene of interest. By binding to the target, the antisense oligonucleotides can prevent translation of the gene into protein via different mechanisms including destruction of the AS-ODN-nucleic acid
Holt, Cathy M +4 more
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The cellular delivery of antisense oligonucleotides and ribozymes
Drug Discovery Today, 2001The design and development of antisense oligonucleotides and ribozymes for the treatment of diseases arising from genetic abnormalities has become a real possibility over the past few years. Improvements in oligonucleotide chemistry have led to the synthesis of nucleic acids that are relatively stable in the biological milieu.
M D., Hughes +4 more
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Intracellular Delivery Strategies for Antisense Phosphorodiamidate Morpholino Oligomers
Antisense and Nucleic Acid Drug Development, 2000Antisense oligonucleotides inhibit gene expression by interfering with transcription, translation, or splicing. They show great potential as gene-specific, nontoxic therapy for a wide variety of diseases. They are also powerful tools to study gene function as well as for validation of therapeutic targets.
C, Ghosh, P L, Iversen
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Nanoparticulate systems for the delivery of antisense oligonucleotides
Advanced Drug Delivery Reviews, 2001Antisense oligonucleotides are molecules that are able to inhibit gene expression being therefore potentially active for the treatment of viral infections or cancer. However, because of their poor stability in biological medium and their weak intracellular penetration, colloidal drugs carriers such as nanoparticles were developed for the delivery of ...
G, Lambert, E, Fattal, P, Couvreur
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Nano drug delivery systems for antisense oligonucleotides (ASO) therapeutics.
Journal of Controlled Release, 2022T. Ramasamy +4 more
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Antisense Oligonucleotide Delivery with Polyhexylcyanoacrylate Nanoparticles as Carriers
Methods, 1999Polyalkylcyanoacrylate nanoparticles are effective colloidal drug carriers and were prepared by an emulsion polymerization process. Antisense oligonucleotides were loaded on the particles by adsorption. A cationic polymer, DEAE-dextran, was incorporated into the particle matrix or a cationic hydrophobic detergent (CTAB) was used to form a lipophilic ...
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In vitro transport and delivery of antisense oligonucleotides
2000A variety of techniques are currently available to enhance the cellular uptake and pharmacological effectiveness of antisense oligonucleotides in the in vitro setting. The choice of technique will depend on the context of investigation, the likelihood of cytotoxity due to the delivery agents, and the ease and convenience of the approach.
J, Hughes +7 more
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