Engineering rotating apical-out airway organoid for assessing respiratory cilia motility [PDF]
iScience, 2022 Summary: Motile cilia project from the airway apical surface and directly interface with inhaled external environment. Owing to cilia’s nanoscale dimension and high beating frequency, quantitative assessment of their motility remains a sophisticated task.
Piyumi Wijesekara +11 more
doaj +9 more sources
Temporal transcriptome profiling of floating apical out chicken enteroids suggest stability and reproducibility [PDF]
Veterinary Research, 2023 Enteroids are miniature self-organising three-dimensional (3D) tissue cultures which replicate much of the complexity of the intestinal epithelium.
Tessa J. Nash +3 more
doaj +9 more sources
Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids [PDF]
Frontiers in Veterinary ScienceDogs are increasingly recognized as valuable large animal models for understanding human intestinal diseases, as they naturally develop conditions similar to those in humans, such as Enterohemorrhagic E.
Shino Yoshida +3 more
doaj +7 more sources
Porcine Intestinal Apical-Out Organoid Model for Gut Function Study [PDF]
Animals, 2022 Pig models provide valuable research information on farm animals, veterinary, and biomedical sciences. Experimental pig gut models are used in studies on physiology, nutrition, and diseases.
Sang-Seok Joo +8 more
doaj +8 more sources
Apical-Out Human Airway Organoids Modeling SARS-CoV-2 Infection [PDF]
Viruses, 2023 The respiratory epithelium, particularly the airway epithelium, is the primary infection site for respiratory pathogens. The apical surface of epithelial cells is constantly exposed to external stimuli including invading pathogens. Efforts have been made
Man Chun Chiu +8 more
doaj +4 more sources
Hypoxia-tolerant apical-out intestinal organoids to model host-microbiome interactions [PDF]
Journal of Tissue Engineering, 2023 Microbiome is an integral part of the gut and is essential for its proper function. Imbalances of the microbiota can be devastating and have been linked with several gastrointestinal conditions. Current gastrointestinal models do not fully reflect the in
Panagiota Kakni +6 more
doaj +5 more sources
Characterization of bovine and ovine basal-out and apical-out ileum organoids [PDF]
Royal Society Open ScienceOrganoids are three-dimensional stem cell-derived structures that differentiate into multiple cell types. Their capacity to self-organize, coupled with the presence of diverse cell types, means that organoids resemble their organ of origin in ...
Ambre F. Chapuis +8 more
doaj +5 more sources
Evaluating protective effects of botanicals under inflammation and oxidative stress in chicken apical-out enteroids [PDF]
Poultry Science, 2023 : Botanicals (BOTs) are well known for their anti-inflammatory and antioxidant activities. They have been widely used as feed additives to reduce inflammation and improve intestinal functions in agricultural animals.
Federico Ghiselli +4 more
doaj +4 more sources
Apical-out airway organoids as a platform for studying viral infections and screening for antiviral drugs [PDF]
Scientific Reports, 2022 Airway organoids are polarized 3D epithelial structures that recapitulate the organization and many of the key functions of the in vivo tissue. They present an attractive model that can overcome some of the limitations of traditional 2D and Air–Liquid ...
Georgios Stroulios +12 more
doaj +5 more sources
Apical-out bovine intestinal organoids as an infection model for Cryptosporidium parvum [PDF]
Current Research in Parasitology and Vector-Borne DiseasesCryptosporidium parvum is a major pathogen responsible for neonatal calf diarrhoea, but research has been hampered due to the lack of in vitro models that can complete the life cycle.
Mathilde Svensen Varegg +4 more
doaj +3 more sources