Results 11 to 20 of about 201,051 (250)

PSC-derived intestinal organoids with apical-out orientation as a tool to study nutrient uptake, drug absorption and metabolism [PDF]

Frontiers in Molecular Biosciences, 2023
Intestinal organoids recapitulate many features of the in vivo gastrointestinal tract and have revolutionized in vitro studies of intestinal function and disease.
Panagiota Kakni, Carmen López-Iglesias, Roman Truckenmüller, Pamela Habibović, Stefan Giselbrecht   +4 more
doaj   +5 more sources

Human apical-out nasal organoids reveal an essential role of matrix metalloproteinases in airway epithelial differentiation [PDF]

Nature Communications
Extracellular matrix (ECM) assembly/disassembly is a critical regulator for airway epithelial development and remodeling. Airway organoid is widely used in respiratory research, yet there is limited study to indicate the roles and mechanisms of ECM ...
Liyue Li, Linyi Jiao, Danni Feng, Yizhang Yuan, Xiaoqian Yang, Jian Li, Dong Jiang, Hexin Chen, Qingxiang Meng, Ruchong Chen, Bixing Fang, Xuenong Zou, Zhenhua Luo, Xiaoyan Ye, Yue Hong, Chun Liu, Chunwei Li   +16 more
doaj   +4 more sources

Protocol to engineer apical-out airway organoids using suspension culture of human airway basal stem cell aggregates [PDF]

STAR Protocols, 2023
Summary: Here we present a protocol to engineer apical-out airway organoids (AOAOs) directly from human airway basal stem cells (hABSCs) using suspension culture of hABSC aggregates on a cell-repellent surface.
Piyumi Wijesekara, Kian Z. Patel, Ellen L. Otto, Phil G. Campbell, Xi Ren   +4 more
doaj   +4 more sources

Apical-out intestinal organoids as an alternative model for evaluating deoxynivalenol toxicity and Lactobacillus detoxification in bovine [PDF]

Scientific Reports
Small intestinal organoids are similar to actual small intestines in structure and function and can be used in various fields, such as nutrition, disease, and toxicity research.
Min Gook Lee, Bo Ram Lee, Poongyeon Lee, Soyoung Choi, Jong-Hui Kim, Mi-Hwa Oh, Jae Gyu Yoo   +6 more
doaj   +4 more sources

Apical-Out Enteroids as an Innovative Model for Necrotizing Enterocolitis. [PDF]

J Surg Res, 2023
Necrotizing enterocolitis (NEC) is a gastrointestinal disease of premature neonates. We previously validated a NEC enteroid model derived from human infant intestinal tissue. Typical enteroid configuration is basolateral-out (BO) without direct access to the luminal (apical) surface.
Liebe H, Schlegel C, Cai X, Golubkova A, Loerke C, Leiva T, Hunter CJ.   +6 more
europepmc   +3 more sources

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis. [PDF]

J Vis Exp, 2022
Necrotizing enterocolitis (NEC) is a devastating disease affecting preterm infants, characterized by intestinal inflammation and necrosis. Enteroids have recently emerged as a promising system to model gastrointestinal pathologies. However, currently utilized methods for enteroid manipulation either lack access to the apical surface of the epithelium ...
Burge K, Wilson A, Chaaban H.
europepmc   +4 more sources

Apical‐out Tubuloids for Accurate Kidney Toxicity Studies

Aggregate
In kidney organoids, typically only the basal membrane is exposed, limiting toxicity assessments of apically transported drugs. Although the reversion of basal‐out organoids has successfully created apical‐out organoids of the intestine and airway, this ...
Yugyeong Lee, Ji Su Hwang, Ziliang Zhai, Kyungwon Park, Ye Seul Son, Dae‐Soo Kim, Seok Chung, Sejoong Kim, Mi‐Young Son, Gwang Lee, Sungsu Park   +10 more
doaj   +2 more sources

Establishment of an Apical-Out Organoid Model for Directly Assessing the Function of Postbiotics. [PDF]

J Microbiol Biotechnol
In vitro organoids that mimic the physiological properties of in vivo organs based on three-dimensional cell cultures overcome the limitations of two-dimensional culture systems. However, because the lumen of a typical intestinal organoid is internal, we used an apical-out intestinal organoid model in which the lumen that absorbs nutrients is outside ...
Cho Y, Sung MH, Kang HT, Lee JH.
europepmc   +3 more sources

Development of apical out trophoblast stem cell derived organoids to model early human pregnancy. [PDF]

iScience
The development of trophoblast organoids has enabled investigation of placental physiology, disease, and early maternal-fetal interactions during a previously restricted stage of pregnancy. A key shortcoming in existing trophoblast organoid methodologies is the non-physiologic position of the syncytiotrophoblast (STB) within the inner portion of the ...
Zhou J, Sheridan MA, Tian Y, Dahlgren KJ, Messler M, Peng T, Zhao A, Ezashi T, Schulz LC, Ulery BD, Roberts RM, Schust DJ.   +11 more
europepmc   +3 more sources

Next-Generation Porcine Intestinal Organoids: an Apical-Out Organoid Model for Swine Enteric Virus Infection and Immune Response Investigations. [PDF]

J Virol, 2020
Intestinal organoids are a newly developed culture system for investigating pathogen-host interactions. Intestinal organoid models have been widely used since their development, because the results obtained from this type of culture model better represent physiological conditions than those from well-established cell lines.
Li Y, Yang N, Chen J, Huang X, Zhang N, Yang S, Liu G, Liu G.   +7 more
europepmc   +5 more sources