Results 81 to 90 of about 991,351 (253)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Apparatus-specific analysis of fluid adhesion measurements

, 2001
Apparatus-specific analysis of fluid adhesion ...
Roger Horn (13091007), B Francis (10456751)   +1 more
core  

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Method and apparatus for measuring electrical conductivity [PDF]

, 2005
The invention relates to a method and apparatus for the measurement of electrical conductivity and in particular the measurement thereof in materials having low electrical conductivity. In particular, although not exclusively, the invention relates to an
Ah Fock, Antonio Louis, Maxwell, Andrew Douglas, Hancock, Nigel Harold   +2 more
core   +1 more source

DESIGN AND ANALYSIS OF A FOUR-DEGREE-OF-FREEDOM HYBRID SPHERICAL SCANNING APPARATUS

, 2007
The design of a novel scanning apparatus used for digitizing dental impression molds is presented. The apparatus consists of a four-degree-of-freedom hybrid spherical linkage capable of moving the dental mold relative to a laser scanner along a curved ...
Scanning Apparatus Chuanqi
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

What is wrong with Remarks on Colour by Ludwig Wittgenstein. Wallgren, T. H. (Ed.). (2023). The Creation of Wittgenstein. Understanding the Roles of Rush Rhees, Elizabeth Anscombe and Georg Henrik von Wright London: Bloomsbury Academy.

Sententiae
Review of Wallgren, T. H. (Ed.). (2023). The Creation of Wittgenstein. Understanding the Roles of Rush Rhees, Elizabeth Anscombe and Georg Henrik von Wright London: Bloomsbury Academy.
Anna-Mariia Kotliarova
doaj   +1 more source

Apparatus

Apparatus and papers from Charles Merbitz's research on behavior monitoring, especially among individuals in ...

core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source