Results 81 to 90 of about 137,316 (288)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The Water Colour Simulator WASI - User manual for version 3

, 2005
The Water Colour Simulator WASI is a software tool for analyzing and simulating the most common types of spectra that are measured by ship-borne optical ...
Gege, Peter
core  

Computational studies of one and two-dimensional photonic microstructures [PDF]

, 2006
This work is concerned with the study of photonic crystals and other topical photonic microstructures, whose optical properties are investigated by solving the Maxwell equations using appropriate theoretical and computational schemes, which are described.
Beggs, Daryl M., Beggs, Daryl M
core  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Dataset on the variability of the light field in coastal waters

Data in Brief
The authors present data on both inherent and apparent optical properties, CTD profiles for the southwestern area of the Bay of Cartagena (Colombia) along a transect of seven stations.
Stella Patricia Betancur-Turizo, Adan Mejia-Trejo, Yerinelys Santos-Barrera, Tatiana Marin-Amado, Erica Paola Zapata-Valezuela, Joaquín Pablo Rivero-Hernández, Rosana del Pilar Adames-Prada   +6 more
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Inherent and apparent optical properties of Lake Peipsi

, 2004
:Lake Peipsi on the border of Estonia and Russia is a large, shallow, biologically very productive lake. It is bordered by many wetland areas. On the basis of underwater radiation measurements and analyses of water samples we have shown that the optical ...
Strömbeck,, N., Paavel,, B., Pierson,, D., Reinart,, A.,   +3 more
core  

Apparent Surface Free Energy of Polymer/Paper Composite Material Treated by Air Plasma

International Journal of Polymer Science, 2017
Surface plasma treatment consists in changes of surface properties without changing internal properties. In this paper composite polymer/paper material is used for production of packaging in cosmetic industry.
Konrad Terpiłowski
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source