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First Detection and Molecular Characterization of Apple Stem Grooving Virus, Apple Chlorotic Leaf Spot Virus, and Apple Hammerhead Viroid in Loquat in Spain [PDF]

open access: yesPlants, 2021
Loquat (Eriobotrya japonica) is an important crop in Spain. To date, only one viral species, apple stem pitting virus (ASPV), has been detected in Spanish loquat orchards. In this study, the presence of additional viruses infecting this crop in Spain was
Celia Canales   +3 more
doaj   +5 more sources

In Silico Apple Genome-Encoded MicroRNA Target Binding Sites Targeting Apple Chlorotic Leaf Spot Virus

open access: yesHorticulturae, 2023
Apple chlorotic leaf spot virus (ACLSV) (genus, Trichovirus; family, Betaflexiviridae) is a widespread, deleterious, and the most damaging pathogen of pome and fruit trees including domesticated apple (Malus × domestica Borkh.), to which it is ...
Muhammad Aleem Ashraf   +3 more
doaj   +5 more sources

Genome Sequences of Three Apple chlorotic leaf spot virus Isolates from Hawthorns in China. [PDF]

open access: yesPLoS ONE, 2016
The genome sequences of Apple chlorotic leaf spot virus (ACLSV) isolates from three accessions of hawthorns (Crataegus pinnatifida) grown at Shenyang Agricultural University were determined using Illumina RNA-seq. To confirm the assembly data from the de
Wei Guo   +5 more
doaj   +3 more sources

Generation of an Infectious Clone of a New Korean Isolate of Apple chlorotic leaf spot virus Driven by Dual 35S and T7 Promoters in a Versatile Binary Vector [PDF]

open access: yesThe Plant Pathology Journal, 2017
The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned
Ik-Hyun Kim   +8 more
doaj   +3 more sources

Molecular Detection of Latent Apple chlorotic leaf spot virus in Elite Mother Plants of Apple. [PDF]

open access: yesIndian J Virol, 2012
Apple chlorotic leaf spot virus (ACLSV; family Betaflexiviridae genus Trichovirus) is one of the economically important latent virus infecting apple (Malus × domestica Borkh.). Reverse transcriptase polymerase chain reaction (RT-PCR) procedures were used to amplify coat protein gene of ACLSV.
Santosh Watpade   +6 more
europepmc   +4 more sources

Comprehensive Virome Profiling of Apple Mosaic Disease-Affected Trees in Iran Using RT-PCR and Next-Generation Sequencing [PDF]

open access: yesViruses
Apples (Malus domestica), one of Iran’s oldest cultivated fruit crops, hold considerable economic importance. In this study, 170 apple leaf samples representing various commercial cultivars were collected across the country.
Anahita Hamedi   +5 more
doaj   +2 more sources

Assessing the de novo assemblers: a metaviromic study of apple and first report of citrus concave gum-associated virus, apple rubbery wood virus 1 and 2 infecting apple in India [PDF]

open access: yesBMC Genomics
Background The choice of de novo assembler for high-throughput sequencing (HTS) data remains a pivotal factor in the HTS-based discovery of viral pathogens.
Zainul A. Khan   +10 more
doaj   +2 more sources

Viruses of Apple Are Seedborne but Likely Not Vertically Transmitted [PDF]

open access: yesViruses
Many viruses occur in apple (Malus domestica (Borkh.)), but no information is available on their seed transmissibility. Here, we report that six viruses infecting apple trees, namely, apple chlorotic leaf spot virus (ACLSV), apple green crinkle ...
Anna Wunsch   +6 more
doaj   +2 more sources

The Effect of Viral Infection on the Growth of HoneySweet GM Plum Trees [PDF]

open access: yesPlants
Plum pox virus (PPV) is one of the most destructive pathogens affecting stone fruit trees. It causes sharka disease and severe yield losses. The genetically modified plum cultivar ‘HoneySweet’ was developed to provide long-lasting resistance to PPV via ...
Petr Komínek   +2 more
doaj   +2 more sources

A comparative template-switching cDNA approach for HTS-based multiplex detection of three viruses and one viroid commonly found in apple trees [PDF]

open access: yesScientific Reports
Exclusion is a keystone of integrated pest management to prevent the introduction of pathogens. U.S. plant quarantine programs employ PCR and high-throughput sequencing (HTS) to test imported plants for viruses and viroids of concern.
Francisco Mosquera-Yuqui   +8 more
doaj   +2 more sources

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