Results 11 to 20 of about 1,895,341 (135)

The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci [PDF]

International Journal of Molecular Sciences
Apple latent spherical virus (ALSV) is widely used as a virus-induced gene silencing (VIGS) vector for function genome study. However, the application of ALSV to soybeans is limited by the resistance of many varieties. In this study, the genetic locus linked to the resistance of a resistant soybean variety Heinong 84 was mapped by high-throughput ...
Xiaofei Cheng, Yong Li, Weiqin Ji
exaly   +5 more sources

Highly efficient inoculation method of apple viruses to apple seedlings [PDF]

, 2010
Virus inoculation to original plants is an important step in research for many reasons. For example, it is used to satisfy Koch’s postulates, to test resistance to viruses in breeding programs, and to analyze gene function by virus vectors etc.
Yoshikawa, N., Sasaki, S., Yamagishi, N.
core   +14 more sources

GoldenBraid-compatible infectious clone of apple latent spherical virus (ALSV) and its use for virus-induced gene silencing

Biologia Plantarum
Virus-induced gene silencing (VIGS) is a technological process in which the expression of a plant target gene is down-regulated by inoculating a plant with a recombinant virus-based vector carrying part of the coding sequence of the target gene ...
Hana HOFFMEISTEROVÁ, Jakub DUŠEK, Emad IBRAHIM, Tomáš MORAVEC, Jiban Kumar KUNDU   +4 more
doaj   +3 more sources

Apple latent spherical virus (ALSV)-induced gene silencing in a medicinal plant, Lithospermum erythrorhizon. [PDF]

Sci Rep, 2020
AbstractLithospermum erythrorhizon is a medicinal plant that produces shikonin, a red lipophilic naphthoquinone derivative that accumulates exclusively in roots. The biosynthetic steps required to complete the naphthalene ring of shikonin and its mechanism of secretion remain unclear.
Izuishi Y, Isaka N, Li H, Nakanishi K, Kageyama J, Ishikawa K, Shimada T, Masuta C, Yoshikawa N, Kusano H, Yazaki K.   +10 more
europepmc   +4 more sources

Virus-induced gene silencing in <i>Dianthus</i> by an apple latent spherical virus vector system. [PDF]

Plant Biotechnol (Tokyo)
For the functional analysis of Dianthus and carnation endogenous genes, we investigated a viral vector derived from the apple latent spherical virus (ALSV) as a tool for reverse genetic analysis. ALSV can infect the aerial parts, such as leaves and flower organs, of Dianthus and carnation plants, without causing viral symptoms. Partial sequences of the
Tanase K, Kasajima I, Yoshikawa N.
europepmc   +4 more sources

Apple chlorotic leaf spot virus [PDF]

, 2016
NYS IPM Type: Fruits IPM Fact SheetApple chlorotic leaf spot virus (ACLSV) infects pome and stone fruits. It can elicit diverse symptoms although, in most cultivars the virus is latent, which means that infected trees do not manifest observable symptoms.
Cieniewicz, Elizabeth, Fuchs, Marc
core   +6 more sources

Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release. [PDF]

Commun Biol, 2020
AbstractPicorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet malleable enough to allow its release. However, molecular mechanisms remain obscure.
Naitow H, Hamaguchi T, Maki-Yonekura S, Isogai M, Yoshikawa N, Yonekura K.   +5 more
europepmc   +4 more sources

Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing. [PDF]

Plant Direct
ABSTRACT Plant virus‐based gRNA delivery systems offer a rapid alternative to stable transformation for CRISPR‐mediated genome editing, but potyvirus‐based platforms in Cas9‐expressing plants are still underexplored. Here, we developed a turnip mosaic virus (TuMV)‐based system for gRNA delivery in Cas9‐expressing Nicotiana benthamiana and tested ...
Khwanbua E, Lappe RR, Bierl AA, Whitham SA.   +3 more
europepmc   +2 more sources

Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release [PDF]

, 2021
Picorna-like plant viruses are non-enveloped RNA spherical viruses of ~30 nm. Part of the survival of these viruses depends on their capsid being stable enough to harbour the viral genome and yet malleable enough to allow its release.
ISOGAI, Masamichi, YONEKURA, Koji, HAMAGUCHI, Tasuku, MAKI-YONEKURA, Saori, YOSHIKAWA, Nobuyuki, NAITO, Hisashi   +5 more
core   +1 more source

Apple Russet Ring and Apple Green Crinkle Diseases: Fulfillment of Koch’s Postulates by Virome Analysis, Amplification of Full-Length cDNA of Viral Genomes, in vitro Transcription of Infectious Viral RNAs, and Reproduction of Symptoms on Fruits of Apple Trees Inoculated With Viral RNAs

Frontiers in Microbiology, 2020
Apple russet ring and apple green crinkle are graft-transmitted diseases first reported more than 60 years ago, but at present, no association between a specific virus (variant) and the disease has been clearly demonstrated.
Chunjiang Li, Hajime Yaegashi, Ryusuke Kishigami, Ayaka Kawakubo, Noriko Yamagishi, Tsutae Ito, Nobuyuki Yoshikawa, Nobuyuki Yoshikawa   +7 more
doaj   +1 more source