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A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity
Applied Sciences, 2022 Plants have evolved defense mechanisms to suppress viral transcription and replication by transcriptional and post-transcriptional gene silencing mediated by virus-derived small interfering RNAs (vsiRNAs).
Hernán de Jesús Villanueva-Alonzo +11 more
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浙江大学学报. 农业与生命科学版, 2008 The term virus-induced gene silencing' (VIGS) was first used to describe the phenomenon of recovery from virus infection, a natural plant antiviral mechanism, which recently has been exploited as a genetic technique involving recombinant viruses with a ...
XU You-ping +4 more
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Molecular Plant-Microbe Interactions, 2002 Transgenic Nicotiana benthamiana expressing the minor coat protein P74 of the phloem-limited Beet western yellows virus (BWYV) exhibited an unusual spatial pattern of post-transcriptional gene silencing (PTGS) when infected with BWYV or related viruses ...
V. Brault +4 more
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Molecular Plant-Microbe Interactions, 2000 Nicotiana benthamiana plants expressing Brome mosaic virus (BMV) p2 protein complemented replication of RNAs1+3 but, surprisingly, supported little or no replication of RNA-2. Despite this, the p2 transgenic plants were able to support systemic migration
Lakshminarayan M. Iyer, Timothy C. Hall
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Efficient Virus-Induced Gene Silencing in Solanum rostratum. [PDF]
PLoS ONE, 2016 Solanum rostratum is a "super weed" that grows fast, is widespread, and produces the toxin solanine, which is harmful to both humans and other animals.
Lan-Huan Meng +5 more
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Molecular Plant-Microbe Interactions, 2003 The ChlH gene, encoding for the H subunit of the magnesium chelatase enzyme, was silenced in Nicotiana bentahamiana plants by virus-induced gene silencing (VIGS), using tobacco mosaic virus (TMV) expression vector.
Jean-Baptiste Hiriart +2 more
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Virus‐induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana
Plant Direct, 2018 Virus‐induced gene silencing (VIGS) is an important forward and reverse genetics method for the study of gene function in many plant species, especially Nicotiana benthamiana.
Muthappa Senthil‐Kumar +15 more
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Chinese Wheat Mosaic Virus-Induced Gene Silencing in Monocots and Dicots at Low Temperature
Frontiers in Plant Science, 2018 Virus-induced gene silencing (VIGS) is an important tool for functional genomics studies in plants. With this method, it is possible to target most endogenous genes and downregulate the messenger RNA (mRNA) in a sequence-specific manner.
Jian Yang +13 more
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Legume Science, 2023 Virus‐induced gene silencing (VIGS) is a functional genomics tool used to determine the function of unknown genes or assess the impact of gene silencing on plant phenotype.
Ruyi Xiong +3 more
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Application and Expansion of Virus-Induced Gene Silencing for Functional Studies in Vegetables
Horticulturae, 2023 Increased consumption of vegetables has been recommended worldwide as a part of a healthy diet; therefore, determining gene function among breeding materials is crucial for vegetable improvement to meet the sustainable development of new vegetable ...
Zheng Wang +7 more
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