Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)
International audiencePlants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from ...
Godfrey Neutelings +5 more
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Defense pathways in Arabidopsis restricting compatible tobamoviruses during infection [PDF]
Virus infected plants often develop strong disease symptoms including leaf deformation, chlorosis, necrosis and growth inhibition. In agriculture systems virus infection can lead to severe yield losses and a better understanding of plant defenses against
Kørner, Camilla Julie
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Catch Me If You Can! RNA Silencing-Based Improvement of Antiviral Plant Immunity
Viruses are obligate parasites which cause a range of severe plant diseases that affect farm productivity around the world, resulting in immense annual losses of yield.
Fatima Yousif Gaffar, Aline Koch
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A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS) [PDF]
Virus-induced gene silencing (VIGS) is a rapid and cost-effective reverse genetic technology that can be used to assess gene function in wheat. This chapter contains a detailed description of how to target wheat meiotic genes by VIGS.
Kanyuka, K. +6 more
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Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules [PDF]
The tospovirus NSs protein was previously shown to suppress the antiviral RNA silencing mechanism in plants. Here the biochemical analysis of NSs proteins from different tospoviruses, using purified NSs or NSs containing cell extracts, is described.
Kormelink, R.J.M. +16 more
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Involvement of cathepsin B in the plant disease resistance hypersensitive response [PDF]
A diverse range of plant proteases are implicated in pathogen perception and in subsequent signalling and execution of disease resistance. We demonstrate, using protease inhibitors and virus-induced gene silencing (VIGS), that the plant papain cysteine ...
Shaw, Jane +49 more
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Virus-induced Gene Silencing (VIGS) in Phalaenopsis Orchids
This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as a silencing vector for floral functional genomics in Phalaenopsis orchids. This protocol is established based on a method created by Lu et al. (2007), and then
Ming-Hsien Hsieh +3 more
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Influence of viral genes on the cell-to-cell spread of RNA silencing [PDF]
The turnip crinkle virus-based vector TCV–GFPDCP had been devised previously to study cell-to-cell and long-distance spread of virus-induced RNA silencing.
Hong, Yiguo +3 more
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Virus-Mediated System for Simultaneous Gene Silencing and Genome Editing in Cotton
Plant viral vectors are powerful tools for the transient expression of exogenous genes, enabling not only virus-induced gene silencing (VIGS) but also virus-induced genome editing (VIGE).
Yufeng Zheng +5 more
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The role of TNRC6 proteins in gene silencing [PDF]
Proteins of the GW182 family have recently emerged as key players in miRNAmediated gene silencing. They have been shown to interact with Argonaute proteins, components of the RISC and are assumed to mediate the repression in metazoa. Three paralogues are
Zipprich, Jakob Theophil
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