Establishing a Virus-Induced Gene Silencing System in Lycoris chinensis. [PDF]
Lycoris is an important plant with both medicinal and ornamental values. However, it does not have an efficient genetic transformation system, which makes it difficult to study gene function of the genus.
Cheng G, Shu X, Wang Z, Wang N, Zhang F.
europepmc +2 more sources
Gene silencing induced by hairpin RNA or virus infection expression is one of the major tools in genetics studies in plants. However, when dealing with essential genes, virus-induced gene silencing (VIGS) and transgenic expression of hairpin RNA could ...
Wang Zhang +6 more
doaj +1 more source
Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing. [PDF]
Rubisco activase (Rca) is an essential photosynthetic enzyme that removes inhibitors from the catalytic sites of the carboxylating enzyme Rubisco. In wheat, Rca is composed of one longer 46 kDa α-isoform and two shorter 42 kDa β-isoforms encoded by the ...
Perdomo JA +4 more
europepmc +2 more sources
Investigation of RNA-induced gene silencing in the fission yeast Schizosaccharomyces pombe [PDF]
PhDRNA interference (RNAi) is a gene regulating system to which can silence genes at both transcriptional and post-transcriptional levels. In this thesis, a ura4-based RNAi selective assay was developed in the fission yeast Schizosaccharomyces pombe ...
Wang, Huan
core +4 more sources
Apple latent spherical virus (ALSV)‑induced gene silencing in a medicinal plant, Lithospermum erythrorhizon [PDF]
Lithospermum erythrorhizon is a medicinal plant that produces shikonin, a red lipophilic naphthoquinone derivative that accumulates exclusively in roots.
NAKANISHI, Kohei +10 more
core +1 more source
Factors influencing Barley Stripe Mosaic Virus-mediated gene silencing in wheat [PDF]
Virus induced gene silencing (VIGS) is a technology that has been used primarily to target the virus genome in infected plants. However, if the virus genome carries inserts derived from the host plant, the system could be employed to target the mRNAs ...
Cakir, Cahid +5 more
core +1 more source
Potato virus X-induced gene silencing in leaves and tubers of potato [PDF]
Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously
Loake, Gary J +17 more
core +1 more source
Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Li, S. +7 more
core +1 more source
Virus-Induced Gene Silencing in Wheat and Related Monocot Species
Advances made in genome sequencing projects and structural genomics are generating large repertoire of candidate genes in plants associated with specific agronomic traits.
Vinay Panwar +5 more
core +1 more source
Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants [PDF]
Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication.
Yule Liu +14 more
core +1 more source

