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Straightforward and affordable agroinfiltration with RUBY accelerates RNA silencing research
Plant Molecular BiologyTransient expression and induction of RNA silencing by agroinfiltration is a fundamental method in plant RNA biology. Here, we introduce a new reporter assay using RUBY, which encodes three key enzymes of the betalain biosynthesis pathway, as a ...
Atsushi Takeda, Kazuki Motomura
exaly +5 more sources
Indonesian Journal of BiotechnologyTransient gene expression is an approach used to study transient genes across various species, with infiltration by Agrobacterium tumefaciens (agroinfiltration) being a commonly used method.
Dionysia Heviarie Primasiwi +2 more
doaj +22 more sources
PLoS ONE, 2022 Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines.
Jordan Demone +7 more
doaj +3 more sources
Plants, 2017 Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency.
Xuejing Wen, Zilong Tan, Wang Yucheng
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Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana [PDF]
Plant Methods, 2018 Background Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite
Karlah Norkunas +3 more
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Agroinfiltration technique for elucidating the functions of strawberry genes in Fragaria vesca. [PDF]
Sci RepUnraveling gene function is crucial for enhancing quality traits in commercially cultivated strawberry (Fragaria × ananassa) through biotechnology.
Thagun C, Kodama Y.
europepmc +4 more sources
Nicotiana benthamiana's Responses to Agroinfiltration, a Treasure Grove of New Avenues to Improve Protein Yields in Plant Molecular Farming. [PDF]
Plant Biotechnol JTransient expression of recombinant proteins in leaves of Nicotiana benthamiana is routinely employed for both basic research and manufacturing of biopharmaceutical products in plants.
Hamel LP.
europepmc +2 more sources
Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter. [PDF]
Hortic ResAgrobacterium-mediated transient transformation is a fundamental tool for plant research and molecular pharming, but its application is often limited by host immune responses, post-transcriptional gene silencing, and the lack of a robust, quantitative ...
Wang T +16 more
europepmc +2 more sources
A Fluorescence‐Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants [PDF]
Plant DirectUpstream open reading frames (uORFs) are regulatory elements present in the 5′ leaders of mRNA that can significantly impact downstream gene expression in eukaryotes. In crop engineering, editing of uORFs can provide an avenue to upregulate expression of
Benjamin E. Haas +7 more
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Frontiers in Plant Science, 2022 Industrial hemp is a diploid (2n = 20), dioecious plant, and an essential source of various phytochemical productions. More than 540 phytochemicals have been described, some of which proved helpful in the remedial treatment of human diseases.
Wayne Curtis, Sairam Rudrabhatla
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