Plants, 2017 Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency.
Huimin Zhao, Zilong Tan, Xuejing Wen
exaly +3 more sources
A Fluorescence‐Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants [PDF]
Plant DirectUpstream open reading frames (uORFs) are regulatory elements present in the 5′ leaders of mRNA that can significantly impact downstream gene expression in eukaryotes. In crop engineering, editing of uORFs can provide an avenue to upregulate expression of
Benjamin E. Haas +7 more
doaj +2 more sources
Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration. [PDF]
, 2018 Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.
Kailemia, Muchena J +5 more
core +10 more sources
Mi-1-mediated resistance to Meloidogyne incognita in tomato may not rely on ethylene but hormone perception through ETR3 participates in limiting nematode infection in a susceptible host. [PDF]
, 2013 Root-knot nematodes, Meloidogyne spp., are important pests of tomato (Solanum lycopersicum) and resistance to the three most prevalent species of this genus, including Meloidogyne incognita, is mediated by the Mi-1 gene. Mi-1 encodes a nucleotide binding
Bhattarai, Kishor K +3 more
core +15 more sources
Frontiers in Sustainable Food Systems, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR) associated protein (CRISPR/Cas9) is an adaptive immune system of bacteria to counter the impending viral pathogen attack.
Ashwini Talakayala +5 more
doaj +1 more source
Agrobacterium-mediated transient gene expression and silencing: a rapid tool for functional gene assay in potato. [PDF]
PLoS ONE, 2009 Potato is the third most important food crop worldwide. However, genetic and genomic research of potato has lagged behind other major crops due to the autopolyploidy and highly heterozygous nature associated with the potato genome.
Pudota B Bhaskar +4 more
doaj +1 more source
Frontiers in Plant Science, 2021 Infectious bursal disease virus is the causative agent of Gumboro disease, a severe infection that affects young chickens and is associated with lymphoid depletion in the bursa of Fabricius.
Alessio Bortolami +11 more
doaj +1 more source
Frontiers in Plant Science, 2021 As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects.
Gongyao Shi +11 more
doaj +1 more source
3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants
Engineering in Life Sciences, 2022 The Australian tobacco plant Nicotiana benthamiana is becoming increasingly popular as a platform for protein production and metabolic engineering. In this system, gene expression is achieved transiently by infiltrating N.
Ling Chuang +4 more
doaj +1 more source
Recent Advances of In Vitro Culture for the Application of New Breeding Techniques in Citrus
Plants, 2020 Citrus is one of the most important fruit crops in the world. This review will discuss the recent findings related to citrus transformation and regeneration protocols of juvenile and adult explants.
Lara Poles +5 more
doaj +1 more source