Improvement strategies for transient gene expression in mammalian cells [PDF]
Applied Microbiology and BiotechnologyMammalian cells are suitable hosts for producing recombinant therapeutic proteins, with Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells being the most commonly used cell lines. Mammalian cell expression system includes stable and transient gene expression (TGE) system, with the TGE system having the advantages of short cycles ...
Tian-Yun Wang
exaly +4 more sources
Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters [PDF]
Plants, 2021 Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites.
Bingqi Wu +6 more
doaj +2 more sources
Transient Expression of cor Gene in Papaver somniferum
BioImpacts, 2011 Introduction: Papaver somniferum is the commercial source of morphine and codeine. The isolation of effective genes involved in the morphine biosynthesis of P.
Bahman Hosseini +6 more
doaj +3 more sources
An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9 [PDF]
PeerJ, 2020 Protoplasts are commonly used in genetic and breeding research. In this study, the isolation of sorghum protoplasts was optimized and applied to transient gene expression and editing by CRISPR/Cas9. The protoplast was most viable in 0.5 M mannitol, which
Ruirui Meng +6 more
doaj +3 more sources
Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions [PDF]
Plants, 2020 A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and ...
Alexander A. Tyurin +3 more
doaj +2 more sources
Transient Gene Expression by Nonintegrating Lentiviral Vectors
Molecular Therapy, 2006 Nonintegrating lentiviral (NIL) vectors were produced from HIV-1-based lentiviral vectors by introducing combinations of mutations made to disable the integrase protein itself and to alter the integrase recognition sequences (att) in the viral LTR. NIL vectors with these novel combinations of mutations were used to transduce the human T lymphoid cell ...
Donald Köhn +2 more
exaly +3 more sources
Transient phenomena in gene expression after induction of transcription. [PDF]
PLoS ONE, 2012 When transcription of a gene is induced by a stimulus, the number of its mRNA molecules changes with time. Here we discuss how this time evolution depends on the shape of the mRNA lifetime distribution.
Carlus Deneke +2 more
doaj +5 more sources
Transient Gene Expression: an Approach for Recombinant Vaccine Production [PDF]
Journal of Medical Microbiology and Infectious Diseases, 2021 The production of recombinant vaccines in green plants is an attractive and promising topic in genetic engineering. However, the stable transformation of green plants is a time-consuming, costly, and labor-intensive practice.
Maziar Habibi-Pirkoohi +2 more
doaj +1 more source
Horticultural Plant Journal, 2023 Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility.
Wenhui Yang +8 more
doaj +1 more source
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration [PDF]
Revista Mvz Cordoba, 2014 Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A.
Paula Díaz T +2 more
doaj +2 more sources