Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions [PDF]
Plants, 2020 A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and ...
Alexander A. Tyurin +3 more
doaj +4 more sources
Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters [PDF]
Plants, 2021 Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites.
Bingqi Wu +6 more
doaj +2 more sources
An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9 [PDF]
PeerJ, 2020 Protoplasts are commonly used in genetic and breeding research. In this study, the isolation of sorghum protoplasts was optimized and applied to transient gene expression and editing by CRISPR/Cas9. The protoplast was most viable in 0.5 M mannitol, which
Ruirui Meng +6 more
doaj +3 more sources
Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana [PDF]
Plant Methods, 2018 Background Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite
Karlah Norkunas +3 more
doaj +2 more sources
Updated transient gene expression protocol in Expi293F cells using PEI [PDF]
Frontiers in Bioengineering and BiotechnologyTransient gene expression (TGE) is commonly used for the rapid production of protein-based therapeutics, including antibodies that require post-translational modifications.
Gustaf Hederoth +7 more
doaj +2 more sources
Improvement strategies for transient gene expression in mammalian cells. [PDF]
Appl Microbiol BiotechnolFu Y +5 more
europepmc +2 more sources
Transient Gene Expression: an Approach for Recombinant Vaccine Production [PDF]
Journal of Medical Microbiology and Infectious Diseases, 2021 The production of recombinant vaccines in green plants is an attractive and promising topic in genetic engineering. However, the stable transformation of green plants is a time-consuming, costly, and labor-intensive practice.
Maziar Habibi-Pirkoohi +2 more
doaj +1 more source
Rhizobium rhizogenes A4-derived strains mediate hyper-efficient transient gene expression in Nicotiana benthamiana and other solanaceous plants. [PDF]
Plant Biotechnol JLopez-Agudelo JC +11 more
europepmc +2 more sources
Horticultural Plant Journal, 2023 Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility.
Wenhui Yang +8 more
doaj +1 more source
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration [PDF]
Revista Mvz Cordoba, 2014 Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A.
Paula Díaz T +2 more
doaj +2 more sources