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Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions [PDF]
Plants, 2020 A large data array on plant gene expression accumulated thanks to comparative omic studies directs the efforts of researchers to the specific or fine effects of the target gene functions and, as a consequence, elaboration of relatively simple and ...
Alexander A. Tyurin +3 more
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Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters [PDF]
Plants, 2021 Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites.
Bingqi Wu +6 more
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Improvement strategies for transient gene expression in mammalian cells [PDF]
Applied Microbiology and BiotechnologyMammalian cells are suitable hosts for producing recombinant therapeutic proteins, with Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells being the most commonly used cell lines. Mammalian cell expression system includes stable and transient gene expression (TGE) system, with the TGE system having the advantages of short cycles ...
Tian-Yun Wang
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An efficient sorghum protoplast assay for transient gene expression and gene editing by CRISPR/Cas9 [PDF]
PeerJ, 2020 Protoplasts are commonly used in genetic and breeding research. In this study, the isolation of sorghum protoplasts was optimized and applied to transient gene expression and editing by CRISPR/Cas9. The protoplast was most viable in 0.5 M mannitol, which
Ruirui Meng +6 more
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Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana [PDF]
Plant Methods, 2018 Background Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite
Karlah Norkunas +3 more
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Updated transient gene expression protocol in Expi293F cells using PEI [PDF]
Frontiers in Bioengineering and BiotechnologyTransient gene expression (TGE) is commonly used for the rapid production of protein-based therapeutics, including antibodies that require post-translational modifications.
Gustaf Hederoth +7 more
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Transient Gene Expression: an Approach for Recombinant Vaccine Production [PDF]
Journal of Medical Microbiology and Infectious Diseases, 2021 The production of recombinant vaccines in green plants is an attractive and promising topic in genetic engineering. However, the stable transformation of green plants is a time-consuming, costly, and labor-intensive practice.
Maziar Habibi-Pirkoohi +2 more
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Horticultural Plant Journal, 2023 Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility.
Wenhui Yang +8 more
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Plant Methods, 2021 Background The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency.
Yongpeng Li +15 more
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Bio-Protocol, 2023 Virus-mediated transient gene overexpression and gene expression silencing can be used to screen gene functions in plants. Sugarcane mosaic virus (SCMV) is a positive strand RNA virus in the Potyviridae family that has been modified to be used as vector ...
Iram Gull, Georg Jander
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