Results 41 to 50 of about 773,655 (299)

A novel and efficient Agrobacterium-mediated transient gene expression in citrus epicotyls and mature stem tissues

open access: yesFruit Research
Agrobacterium-mediated transient gene expression is a powerful technique for rapidly evaluating gene expression in higher plants. This study aimed to improve transient expression levels of T-DNA genes in citrus by investigating the effects of various ...
Yanjun Li   +6 more
doaj   +1 more source

The Catalytic and Non-catalytic Functions of the Brahma Chromatin-Remodeling Protein Collaborate to Fine-Tune Circadian Transcription in Drosophila. [PDF]

open access: yes, 2015
Daily rhythms in gene expression play a critical role in the progression of circadian clocks, and are under regulation by transcription factor binding, histone modifications, RNA polymerase II (RNAPII) recruitment and elongation, and post-transcriptional
Chiu, Joanna C   +4 more
core   +3 more sources

Expression of Recombinant Factor IX Using the Transient Gene Expression Technique

open access: yesResearch in Molecular Medicine, 2018
Background: Pilot and large-scale production of recombinant proteins require the presence of stable clones, but the process of selecting stable clones is time consuming.
Jafar Vatandoost, Mohammad Amin Azimifar
doaj   +1 more source

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

open access: yesPlant Methods, 2018
Background Genetically modified cotton accounts for 64% of the world’s cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors Gossypium raimondii and Gossypium arboreum as well as the cultivated Gossypium ...
Haipeng Li   +7 more
doaj   +1 more source

Suitability of transiently expressed antibodies for clinical studies: product quality consistency at different production scales

open access: yesmAbs, 2022
Transgenic human monoclonal antibodies derived from humanized mice against different epitopes of the Middle East respiratory syndrome coronavirus (MERS-CoV), and chimeric llama-human bispecific heavy chain-only antibodies targeting the Rift Valley fever ...
Sara Rodriguez-Conde   +15 more
doaj   +1 more source

Synthetic chemical inducers and genetic decoupling enable orthogonal control of the rhaBAD promoter [PDF]

open access: yes, 2016
External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E.
Heap, JT, Kelly, C
core   +1 more source

Organization and transient expression of the gene for human U11 snRNA

open access: yesGene expression, 1991
The nucleotide sequence of U11 small nuclear RNA, a minor U RNA from HeLa cells, was determined. Computer analysis of the sequence (135 residues) predicts two strong hairpin loops which are separated by seventeen nucleotides containing an Sm binding site (AAUUUUUUGG).
Suter-Crazzolara, C., Keller, W.
openaire   +4 more sources

Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus [PDF]

open access: yes, 1996
The methyl-CpG binding domain (MBD) proteins are key molecules in the interpretation of DNA methylation signals leading to gene silencing. We investigated their binding specificity at the constitutively methylated region of a CpG island containing the ...
Auriol, Emilie   +3 more
core   +2 more sources

CRISPR/Cas9 mediated editing of the Quorn fungus Fusarium venenatum A3/5 by transient expression of Cas9 and sgRNAs targeting endogenous marker gene PKS12

open access: yesFungal Biology and Biotechnology, 2021
Background Gene editing using CRISPR/Cas9 is a widely used tool for precise gene modification, modulating gene expression and introducing novel proteins, and its use has been reported in various filamentous fungi including the genus Fusarium.
Fiona M. Wilson, Richard J. Harrison
doaj   +1 more source

Transient-mediated fate determination in a transcriptional circuit of HIV [PDF]

open access: yes, 2007
Steady-state behavior and bistability have been proposed as mechanisms for decision-making in gene circuits. However, transient gene expression has also been proposed to control cell fate with the decision arbitrated by the lifetime of the expression ...
Leor Weinberger   +2 more
core   +1 more source

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