Results 41 to 50 of about 5,757,325 (187)

Expression of Recombinant Factor IX Using the Transient Gene Expression Technique

Research in Molecular Medicine, 2018
Background: Pilot and large-scale production of recombinant proteins require the presence of stable clones, but the process of selecting stable clones is time consuming.
Jafar Vatandoost, Mohammad Amin Azimifar
doaj   +1 more source

Techniques for clustering gene expression data [PDF]

, 2008
Many clustering techniques have been proposed for the analysis of gene expression data obtained from microarray experiments. However, choice of suitable method(s) for a given experimental dataset is not straightforward. Common approaches do not translate
Kerr, Gráinne, Crane, Martin, M. Crane, H. J. Ruskin, Doolan, Padraig, Ruskin, Heather J., G. Kerr, P. Doolan   +7 more
core   +2 more sources

Enhanced expression of tissue plasminogen activator via gene silencing suppressor strategy using P19 protein [PDF]

مجله بیوتکنولوژی کشاورزی, 2016
Human tissue-type plasminogen activator (tPA) is one of the most important therapeutically proteins involved in the breakdown of blood clots of brain and heart blood vessels following stroke.
Mahshid Amiri, Mokhtar Jalali Javaran, Parastou Ehsani, Rahim Haddad   +3 more
doaj   +1 more source

Transient Gene Expression in Intact and Organized Rice Tissues [PDF]

The Plant Cell, 1990
Regulated gene expression of chimeric genes has been studied extensively in electroporated protoplasts. The applicability of these assays is limited, however, because protoplasts are not always physiologically identical to the cells from which they are derived. We have developed a procedure to electroporate DNA into intact and organized leaf structures
R. A., Dekeyser, B., Claes, RMU., De Rycke, M. E., Habets, M. C., Van Montagu, A. B., Caplan   +5 more
openaire   +2 more sources

A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Plant Methods, 2018
Background Genetically modified cotton accounts for 64% of the world’s cotton growing area (22.3 million hectares). The genome sequencing of the diploid cotton progenitors Gossypium raimondii and Gossypium arboreum as well as the cultivated Gossypium ...
Haipeng Li, Kun Li, Yutao Guo, Jinggong Guo, Kaiting Miao, Jose R. Botella, Chun-Peng Song, Yuchen Miao   +7 more
doaj   +1 more source

Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker [PDF]

, 2009
Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green ...
Yu, X., Li, W., Li, J., Zhang, N., Liang, X., Zhang, X., Gong, P., Ma, G., Ryan, U.M.   +8 more
core  

A Brassica exon array for whole-transcript gene expression profiling [PDF]

, 2010
Affymetrix GeneChip® arrays are used widely to study transcriptional changes in response to developmental and environmental stimuli. GeneChip® arrays comprise multiple 25-mer oligonucleotide probes per gene and retain certain advantages over direct ...
King Graham J., Broadley, MR, May Sean T., John P. Hammond, Bowen, H. C., Bowen, HC, Hammond, J. P., Lochlainn, Seosamh O., Neil S Graham, Philip J White, Graham J. King, Broadley, Martin R., Ó Lochlainn Seosamh, May, ST, Hammond, John P., Graham, NS, Love Christopher G., Christopher G Love, Helen C. Bowen, Graham, N. S., Sean T. May, Philip J. White, Christopher G. Love, Graham, Neil S., O Lochlainn, S., Hammond John P., White, PJ, Graham J King, Ó Lochlainn, Seosamh, May, S. T., Hammond, JP, Love, Christopher G., Love, CG, Helen C Bowen, John P Hammond, Seosamh O Lochlainn, King, GJ, King, G. J., King, Graham J., Broadley, M. R., Broadley Martin R., May, Sean T., Bowen Helen C., Martin R. Broadley, Sean T May, Martin R Broadley, White, P. J., King , Graham J., Love, C. G., Neil S. Graham, White Philip J., White, Philip J., Graham Neil S., Lochlainn, SO, Bowen, Helen C.   +54 more
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Transient expression of chicken alpha interferon gene in lettuce [PDF]

Journal of Zhejiang University SCIENCE B, 2008
We investigated the possibility of producing chicken alpha interferon (ChIFN-alpha) in transgenic plants. The cDNA encoding ChIFN-alpha was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-alpha gene was correctly transcribed and translated in the lettuce plants according to RT-PCR ...
Li, Song, De-gang, Zhao, Yong-jun, Wu, Yi, Li   +3 more
openaire   +2 more sources

Transient-mediated fate determination in a transcriptional circuit of HIV [PDF]

, 2007
Steady-state behavior and bistability have been proposed as mechanisms for decision-making in gene circuits. However, transient gene expression has also been proposed to control cell fate with the decision arbitrated by the lifetime of the expression ...
Michael Simpson, Roy D. Dar, Leor Weinberger   +2 more
core  

Carbon-nanotube for Transient Expression in Rice Calli

Hayati Journal of Biosciences, 2022
Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency.
Paphawarin Pinyokham, Kamolwan Khianchaikhan, Pongsakorn Sunvittayakul, Supachai Vuttipongchaikij, Piyama Tasanasuwan, Chatchawan Jantasuriyarat   +5 more
doaj   +1 more source