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Comparative Analysis of Plant Transient Expression Vectors for Targeted N-Glycosylation [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Alexandra Castilho +2 more
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Transient Expression Assay in Strawberry Fruits [PDF]
BIO-PROTOCOL, 2019 Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions.
Mengting Pi, Qi Gao, Chunying Kang
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Transient expressions of synthetic biology in plants
Current Opinion in Plant Biology, 2014 Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes.
Sainsbury, Frank, Lomonossoff, George P.
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Transient Mesp1 expression [PDF]
Transcription, 2013 Mesp1 sits on the tip of the cardiac regulatory hierarchy, recent evidences support that it is regulated by stem cell factor Oct4, early gastrulation signal canonical Wnts and a couple of T-box factors, T and Eomes. With other transcription factors, Mesp1 programs/reprograms human cells toward cardiomyocytes.
Yu, Liu, Robert J, Schwartz
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Transient protein expression systems in plants and their applications
Plant Biotechnology, 2021 The production of recombinant proteins is important in academic research to identify protein functions. Moreover, recombinant enzymes are used in the food and chemical industries, and high-quality proteins are required for diagnostic, therapeutic, and pharmaceutical applications.
Nosaki, Shohei +3 more
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Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants [PDF]
Plant Molecular Biology Reporter, 2013 Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose.
Shah, Kausar Hussain +2 more
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EXPRESS Transient Ischemic Attack Study [PDF]
Stroke, 2008 Marc Fisher MD Kennedy Lees MD Section Editors: Retinal and cerebral transient ischemic attacks (TIAs) are a falsely benign form of brain attack. After a TIA, the risk of completed stroke is up to 8% within the first 8 to 15 days.1,2 TIAs precede a completed stroke in as many as 25% of patients with stroke.3 Because diffusion-weighted imaging shows a
Pierre, Amarenco, Oscar, Benavente
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DsRed2 transient expression in Culex quinquefasciatus mosquitoes [PDF]
Memórias do Instituto Oswaldo Cruz, 2013 Culex quinquefasciatus mosquitoes have been successfully genetically modified only once, despite the efforts of several laboratories to transform and establish a stable strain. We have developed a transient gene expression method, in Culex, that delivers plasmid DNA directly to the mosquito haemolymph and additional tissues.
Wilke, Andre Barretto Bruno +3 more
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In Planta Transient Expression Systems for Monocots
, 2015 The analysis of gene function in many monocotyledonous species has been held back to a certain extent by the difficulties of stable gene transformation. This chapter discusses the increasing use of transient in planta expression systems available for both RNA interference (RNAi)-mediated downregulation and overexpression of target genes of interest in ...
Lee, W-S. +2 more
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Transient Expression Systems in Plants: Potentialities and Constraints [PDF]
, 2016 Plants have been used from old to extract and isolate by different means the products of interest that they store. In recent years new techniques have emerged that allow the use of plants as factories to overexpress transiently and often efficiently, specific genes of interest, either endogenous or foreign, in their native form or modified.
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