Results 11 to 20 of about 665,407 (262)

Production and Purification of Virus-Like Particles by Transient Expression in Plants [PDF]

, 2023
Transient expression in plants has become a useful production system for virus-like particle (VLP) expression. High yields and flexible approaches to assembling complex VLPs, combine with ease of scale-up and inexpensive reagents to provide an attractive
Esquirol, L, McNeale, D, Sainsbury, F, Venturi, M   +3 more
core   +1 more source

Plant viruses as scaffolds for the presentation of vaccine epitopes

Biologia Plantarum, 2017
Within the last two decades, plant viral vectors have emerged as an excellent tool for the expression of foreign peptides and proteins. Virus particles carrying foreign antigenic epitopes present some interesting advantages for vaccine design and other ...
H. Plchova, N. Cerovska, P. Vaculik, T. Moravec   +3 more
doaj   +1 more source

Transiently Expressed Mistletoe Lectin II in Nicotiana benthamiana Demonstrates Anticancer Activity In Vitro

Molecules, 2020
Mistletoe (Viscum album) extracts have been used as alternative and complementary therapeutic preparations in multiple cancers for decades. Mistletoe lectins (ML-I, ML-II, and ML-III) are considered to be the main anticancer components of such ...
Milena Mazalovska, J. Calvin Kouokam
doaj   +1 more source

Automation of large scale transient protein expression in mammalian cells [PDF]

, 2011
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline.
Susan Daenke, Jones, M, E. Yvonne Jones, Siebold, Christian, Daenke, Susan, Christian Siebold, Bishop, Benjamin, Margaret Jones, Clay, Jordan E, Stuart, David I., A. Radu Aricescu, Zhao, Y, Benjamin Bishop, Aricescu, Alexandru R, Radu Aricescu, A, Stuart, David, Yvonne Jones, E, Weixian Lu, Stuart, David I, Clay, JE, Daenke, S, Aricescu, Alexandru, Bishop, B, Jones, EY, Clay, Jordan E., Yuguang Zhao, Jones, Margaret, Lu, Weixian, David I. Stuart, Radu Aricescu, A., Yvonne Jones, E., Aricescu, AR, Jones, Edith, Jordan E. Clay, Zhao, Yuguang, Jones, E Yvonne, Lu, W, Siebold, C, Stuart, DI   +38 more
core   +1 more source

Virus-mediated transient expression techniques enable gene function studies in black-grass [PDF]

, 2020
Even though considerable progress has been made in weed ecology, weed molecular biology has been hindered by an inability to genetically manipulate weeds.
Macgregor, D., Kanyuka, K., Mellado-Sanchez, M., Mcdiarmid, F., Cardoso, V.   +4 more
core   +1 more source

Infiltration with Agrobacterium tumefaciens Induces Host Defense and Development-Dependent Responses in the Infiltrated Zone

Molecular Plant-Microbe Interactions, 2008
Despite the widespread use of Agrobacterium tumefaciens to transfer genes into plant systems, host responses to this plant pathogen are not well understood.
Gail J. Pruss, Eugene W. Nester, Vicki Vance   +2 more
doaj   +1 more source

Development of a Virus-Based Reporter System for Functional Analysis of Plant rRNA Gene Promoter

Frontiers in Microbiology, 2021
Reporter gene-based expression systems have been intensively used in plants for monitoring the activity of gene promoters. However, rRNA transcripts are unable to efficiently express a reporter gene due to a lack of a 5' cap.
Li Xu, Zhiying Li, Zhiying Li, Sheng Wang, Sheng Wang   +4 more
doaj   +1 more source

Expression and Functional Evaluation of Recombinant Anti-receptor Activator of Nuclear Factor Kappa-B Ligand Monoclonal Antibody Produced in Nicotiana benthamiana

Frontiers in Plant Science, 2021
Denosumab, an anti-receptor activator of nuclear factor-kappa B ligand antibody (anti-RANKL), is a fully human monoclonal antibody (mAb) available for the treatment of osteoporosis.
Wanuttha Boonyayothin, Wanuttha Boonyayothin, Sirorut Sinnung, Balamurugan Shanmugaraj, Balamurugan Shanmugaraj, Yoshito Abe, Richard Strasser, Prasit Pavasant, Prasit Pavasant, Waranyoo Phoolcharoen, Waranyoo Phoolcharoen   +10 more
doaj   +1 more source

Functional modulation of the transient outward current Ito by KCNE beta-subunits and regional distribution in human non-failing and failing hearts [PDF]

, 2006
Objectives: The function of Kv4.3 (KCND3) channels, which underlie the transient outward current I,, in human heart, can be modulated by several accessory subunits such as KChIP2 and KCNE1-KCNE5.
Cotella, D., Varró, András, Ravens, U., Wettwer, E., Radicke, S., Banse, U., Jost, Norbert, Graf, E., Tseng, G.   +8 more
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Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops

Frontiers in Plant Science, 2021
Versatile protoplast platforms greatly facilitate the development of modern botany. However, efficient protoplast-based systems are still challenging for numerous horticultural plants and crops.
Rui Ren, Rui Ren, Jie Gao, Dongmei Yin, Kai Li, Chuqiao Lu, Sagheer Ahmad, Yonglu Wei, Jianpeng Jin, Genfa Zhu, Fengxi Yang   +10 more
doaj   +1 more source