Results 11 to 20 of about 1,253,450 (306)

Improving transient expression in N. benthamiana by suppression of the Nb-SABP2 and Nb-COI1 plant defence response related genes [PDF]

open access: yesFrontiers in Plant Science
Currently transient expression is one of the preferred plant-based technologies for recombinant protein manufacturing, particularly in respect to pharmaceutically relevant products.
Lilya Kopertekh
doaj   +2 more sources

Production of two SARS-CoV-2 neutralizing antibodies with different potencies in Nicotiana benthamiana

open access: yesFrontiers in Plant Science, 2022
Monoclonal antibodies are considered to be highly effective therapeutic tools for the treatment of mild to moderate COVID-19 patients. In the present work, we describe the production of two SARS-CoV-2 human IgG1 monoclonal antibodies recognizing the ...
Rachele Frigerio   +12 more
doaj   +1 more source

Transient Expression in HEK-293 Cells in Suspension Culture as a Rapid and Powerful Tool: SARS-CoV-2 N and Chimeric SARS-CoV-2N-CD154 Proteins as a Case Study

open access: yesBiomedicines, 2023
In a previous work, we proposed a vaccine chimeric antigen based on the fusion of the SARS-CoV-2 N protein to the extracellular domain of the human CD40 ligand (CD154). This vaccine antigen was named N-CD protein and its expression was carried out in HEK-
Thailin Lao   +9 more
doaj   +1 more source

Nutritional Enrichment of Plant Leaves by Combining Genes Promoting Tocopherol Biosynthesis and Storage

open access: yesMetabolites, 2023
The enrichment of plant tissues in tocochromanols (tocopherols and tocotrienols) is an important biotechnological goal due to their vitamin E and antioxidant properties.
Luca Morelli   +5 more
doaj   +1 more source

Design of a Diagnostic Immunoassay for Aflatoxin M1 Based on a Plant-Produced Antibody

open access: yesToxins, 2022
A new green competitive ELISA for aflatoxin M1 quantification in raw milk was developed. This diagnostic tool is based on an anti AFM1 mAb produced by plant molecular farming in alternative to classical systems.
Cristina Capodicasa   +4 more
doaj   +1 more source

Rapid analysis of strigolactone receptor activity in a Nicotiana benthamiana dwarf14 mutant

open access: yesPlant Direct, 2022
DWARF14 (D14) is an ɑ/β‐hydrolase and receptor for the plant hormone strigolactone (SL) in angiosperms. Upon SL perception, D14 works with MORE AXILLARY GROWTH2 (MAX2) to trigger polyubiquitination and degradation of DWARF53(D53)‐type proteins in the ...
Alexandra R. F. White   +3 more
doaj   +1 more source

COVID-19 Crisis: How Can Plant Biotechnology Help?

open access: yesPlants, 2021
The emergence of the COVID-19 pandemic has led to significant public health crisis all over the world. The rapid spreading nature and high mortality rate of COVID-19 places a huge pressure on scientists to develop effective diagnostics and therapeutics ...
Md. Jahidul Islam Shohag   +5 more
doaj   +1 more source

Transient Expression Assay in Strawberry Fruits [PDF]

open access: yesBIO-PROTOCOL, 2019
Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions.
Mengting Pi, Qi Gao, Chunying Kang
openaire   +3 more sources

Plant viruses as scaffolds for the presentation of vaccine epitopes

open access: yesBiologia Plantarum, 2017
Within the last two decades, plant viral vectors have emerged as an excellent tool for the expression of foreign peptides and proteins. Virus particles carrying foreign antigenic epitopes present some interesting advantages for vaccine design and other ...
H. Plchova   +3 more
doaj   +1 more source

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