Different 3' end regions strongly influence the level of gene expression in plant cells [PDF]
, 1989 We have investigated the functional role of a 3' end region on the expression of a reporter gene in plant cells. In stably transformed plants, expression of the reporter gene without a plant gene 3' end is variable and depends on the fortuitous presence ...
Ingelbrecht, Ivan +4 more
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Functional expression of TRPV4 channels in human collecting duct cells: implications for secondary hypertension in diabetic nephropathy [PDF]
, 2012 Background. The Vanilloid subfamily of transient receptor potential (TRPV) ion channels has been widely implicated in detecting osmotic and mechanical stress.
Bland, Rosemary +2 more
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Transient response of a quantum wave to an instantaneous potential step switching [PDF]
, 2002 The transient response of a stationary state of a quantum particle in a step potential to an instantaneous change in the step height (a simplified model for a sudden bias switch in an electronic semiconductor device) is solved exactly by means of a ...
Baute A D +13 more
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Transient expressions of synthetic biology in plants
Current Opinion in Plant Biology, 2014 Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes.
Sainsbury, Frank, Lomonossoff, George P.
openaire +4 more sources
Transient Mesp1 expression [PDF]
Transcription, 2013 Mesp1 sits on the tip of the cardiac regulatory hierarchy, recent evidences support that it is regulated by stem cell factor Oct4, early gastrulation signal canonical Wnts and a couple of T-box factors, T and Eomes. With other transcription factors, Mesp1 programs/reprograms human cells toward cardiomyocytes.
Yu, Liu, Robert J, Schwartz
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Synthetic chemical inducers and genetic decoupling enable orthogonal control of the rhaBAD promoter [PDF]
, 2016 External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E.
Heap, JT, Kelly, C
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Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants [PDF]
Plant Molecular Biology Reporter, 2013 Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose.
Shah, Kausar Hussain +2 more
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Frontiers in Plant Science, 2014 Artificial microRNAs (amiRNAs) have become an important tool to assess gene functions due to their high efficiency and specificity to decrease target gene expression.
Shi eYu, Guillaume ePilot
doaj +1 more source
Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes [PDF]
Archives of Biological Sciences, 2010 Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner.
Bratić Ana M. +4 more
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Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco [PDF]
, 2014 The proposed clinical trial in Africa of VRC01, a potent broadly neutralizing antibody (bNAb) capable of neutralizing 91% of known HIV-1 isolates, raises concerns about testing a treatment which will be too expensive to be accessible by the most ...
EMEA +4 more
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