Results 1 to 10 of about 686,707 (295)
, 2011 Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
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, 2019 Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
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A Mechanistic Dissection of Polyethylenimine Mediated Transfection of Chinese Hamster Ovary Cells [PDF]
, 2013 Biopharmaceutical production through transient gene expression (TGE) is used within industry for the rapid supply of product for early stage testing.
Mozley, O L
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Molecular distribution in intradermal injection for transfer and delivery of therapeutics
Frontiers in Drug Delivery, 2023 Intradermal (ID) injection is a technique widely used in laboratorial and clinical applications. The boundary of the dome-like bleb formed during injection is assumed to represent the lateral extent of the injected material.
Emran O. Lallow +13 more
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Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods [PDF]
Veterinary World, 2022 Background and Aim: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/ Cas9) system ...
Quynh Anh Le +8 more
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Tailoring optical fibers for cell transfection [PDF]
, 2012 Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane.
Ma, Nan
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Cationic lipid-mediated intracellular delivery of antibodies into live cells
BioTechniques, 2008 The ability to introduce antibodies to live cells opens new insights to a wide range of applications, such as protein intracellular trafficking studies, protein interference studies with blocking antibodies, and live immunolabeling or protein ...
Claire O. Weill +2 more
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Pharmaceutics, 2022 Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport.
Tina Vindiš +5 more
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Molecular Therapy: Nucleic Acids, 2013 RNA interference (RNAi) is a major tool for basic and applied investigations. However, obtaining RNAi data that have physiological significance requires investigation of regulations and therapeutic strategies in appropriate in vivo settings.
Sylvie Remaud +11 more
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Infrared Laser-Based Single Cell Permeabilization by Plasma Membrane Temperature Gradients
Membranes, 2022 Single cell microinjection provides precise tuning of the volume and timing of delivery into the treated cells; however, it also introduces workflow complexity that requires highly skilled operators and specialized equipment.
Allen L. Garner +2 more
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