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An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro
Frontiers in Bioengineering and Biotechnology, 2021 Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa +7 more
doaj +2 more sources
Molecules, 2018 Non-viral vector-mediated transfection is a core technique for in vitro screening of oligonucleotides. Despite the growing interests in the development of oliogonucleotide-based drug molecules in recent years, a comprehensive comparison of the ...
Tao Wang, Leon M Larcher, Lixia Ma
exaly +2 more sources
A ‘poly-transfection’ method for rapid, one-pot characterization and optimization of genetic systems
Nucleic Acids Research, 2019 Biological research is relying on increasingly complex genetic systems and circuits to perform sophisticated operations in living cells. Performing these operations often requires simultaneous delivery of many genes, and optimizing the stoichiometry of ...
Jeremy J Gam +2 more
exaly +2 more sources
Mammalian cell transfection: the present and the future
Analytical and Bioanalytical Chemistry, 2010 Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical.
Tae Kyung Kim +2 more
exaly +2 more sources
Advances in Materials, 2023 The transfection potency of lipid nanoparticle (LNP) mRNA systems is critically dependent on the ionizable cationic lipid component. LNP mRNA systems composed of optimized ionizable lipids often display distinctive mRNA‐rich “bleb” structures.
Miffy H Y Cheng +12 more
semanticscholar +1 more source
Transfection types, methods and strategies: a technical review
PeerJ, 2021 Transfection is a modern and powerful method used to insert foreign nucleic acids into eukaryotic cells. The ability to modify host cells’ genetic content enables the broad application of this process in studying normal cellular processes, disease ...
Z. Chong, S. Yeap, W. Ho
semanticscholar +1 more source
Molecular distribution in intradermal injection for transfer and delivery of therapeutics
Frontiers in Drug Delivery, 2023 Intradermal (ID) injection is a technique widely used in laboratorial and clinical applications. The boundary of the dome-like bleb formed during injection is assumed to represent the lateral extent of the injected material.
Emran O. Lallow +13 more
doaj +1 more source
Protamine-Based Strategies for RNA Transfection
Pharmaceutics, 2021 Protamine is a natural cationic peptide mixture mostly known as a drug for the neutralization of heparin and as a compound in formulations of slow-release insulin.
N. Jarzebska +4 more
semanticscholar +1 more source
Transfection of Platyhelminthes [PDF]
BioMed Research International, 2015 Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Moguel, Bárbara +3 more
openaire +2 more sources
A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing
Theranostics, 2020 Gene editing is a versatile technique in biomedicine that promotes fundamental research as well as clinical therapy. The development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) as a genome editing machinery has accelerated the ...
A. K. Fajrial +4 more
semanticscholar +1 more source