Results 1 to 10 of about 182,543 (292)

Single cell electroporation on chip [PDF]

, 2006
In this thesis the results of the development of microfluidic cell trap devices for single cell electroporation are described, which are to be used for gene transfection.
Valero, Ana
core   +2 more sources

A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development

STAR Protocols, 2021
Summary: In vivo electroporation has become a key technique to study genetic mechanisms of brain development. However, electroporation of the embryonic pallium in oviparous species, interesting for evolutionary studies but distinct from in utero ...
Adrián Cárdenas, Víctor Borrell
doaj   +1 more source

Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method

Journal of Experimental Life Science, 2017
Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation.
Diana Aisyah, Agoes Soeprijanto, Yuni Kilawati   +2 more
doaj   +1 more source

Analyzing the effects of surface distribution of pores in cell electroporation for a cell membrane containing cholesterol

, 2008
This paper presents a model and numerical analysis (simulations) of transmembrane potential induced in biological cell membrane under the influence of externally applied electric field (i.e., electroporation).
DeBruin K A, DeBruin K A, Hibino M, Jamieson G, Karatekin E, Krassowska W, Pandit B Vidyasagar, Pratip Shil, Salil Bidaye, Shil P, Shil P, Smith K C   +11 more
core   +1 more source

One-year Follow-up Results of Transperineal Biopsy For Patients Undergoing Irreversible Electroporation Treatment in Localized Prostate Cancer

Urology Research and Practice, 2023
Objective: This article reports on the early results of a 1-year follow-up study investigating the efficacy of irreversible electroporation in the treatment of localized prostate cancer. Methods: The study included 18 out of 40 patients diagnosed with
Şükrü Ali Altan, Pınar Güleryüz Kızıl, Nefise Çağla Tarhan, Oztug Adsan   +3 more
doaj   +1 more source

The Quality of Ornamental Japanese Carp (Cyprinus carpio var. Koi) After Electroporation as a Gene Material Transfer Method

Journal of Experimental Life Science, 2016
Spermatozoa are male gametes that have an advantage in acting as gene transfer media (SMGT), because in transferring genetic material, sperm uses relatively natural vectors.
Saidah Luthfiyah, Agoes Soeprijanto, Yuni Kilawati   +2 more
doaj   +1 more source

Testis electroporation coupled with autophagy inhibitor to treat non-obstructive azoospermia

Molecular Therapy: Nucleic Acids, 2022
Infertility affects 10%–20% of the population in most countries, with approximately half of those cases resulting from male infertility. Although millions of infertile men are able to have children with the assistance of reproductive technology ...
Liying Wang, Chao Liu, Huafang Wei, Yingchun Ouyang, Mingzhe Dong, Ruidan Zhang, Lina Wang, Yinghong Chen, Yanjie Ma, Mengmeng Guo, Yang Yu, Qing-Yuan Sun, Wei Li   +12 more
doaj   +1 more source

Activation of Ras-ERK pathway by Fgf8 and its downregulation by Sprouty2 for the isthmus organizing activity [PDF]

, 2008
In the previous studies, we showed that strong Fgf8 signaling activates the Ras-ERK pathway to induce cerebellum. Here, we show importance of negative regulation of this pathway.
Asuka Suzuki-Hirano, Harukazu Nakamura, Tatsuya Sato   +2 more
core   +1 more source

Cell-Penetrating Peptides, Electroporation, and Drug Delivery

, 2010
Certain short polycations, such as TAT and oligoarginine, rapidly pass through the plasma membranes of mammalian cells by a mechanism called transduction, as well as by endocytosis and macropinocytosis.
Cahill, Kevin
core   +1 more source

Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA [PDF]

, 2008
Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested.
Nierop Groot, M.N., Nieboer, F., Abee, T.   +2 more
core   +1 more source