Results 11 to 20 of about 133,618 (201)
Cold Spring Harbor Protocols, 2019 Electroporation is a process in which brief electrical pulses create transient pores in the plasma membrane that allow nucleic acids to enter the cellular cytoplasm. Here, we provide information on the history, mechanism, and optimization of electroporation.
Priti, Kumar +2 more
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Drug Design, Development and Therapy, 2017 Shun Feng,1,* Lijun Zhu,1,* Zhisheng Huang,2 Haojia Wang,1 Hong Li,1 Hua Zhou,3 Linlin Lu,1 Ying Wang,1 Zhongqiu Liu,1,3 Liang Liu1,3 1International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou ...
Feng S +9 more
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Electroporation of Mycobacteria [PDF]
Journal of Visualized Experiments, 2008 High efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall, but is compounded by the fact that most molecular techniques have been developed for distantly-related species such as Escherichia coli and Bacillus subtilis. In
Renan, Goude, Tanya, Parish
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Effect of calcium electroporation in combination with metformin in vivo and correlation between viability and intracellular ATP level after calcium electroporation in vitro. [PDF]
PLoS ONE, 2017 BACKGROUND:Calcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses.
Stine Krog Frandsen, Julie Gehl
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APL Bioengineering, 2023 Electroporation shows great potential in biology and biomedical applications. However, there is still a lack of reliable protocol for cell electroporation to achieve a high perforation efficiency due to the unclear influence mechanism of various factors,
Qiongyao Mou +4 more
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Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel
Micromachines, 2020 The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics ...
Yifei Ye +7 more
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The Journal of Reproduction and Development, 2022 Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing ...
Yui WAKE +3 more
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A transportable, inexpensive electroporator for in utero electroporation [PDF]
Development, Growth & Differentiation, 2015 Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude.
Torsten, Bullmann +3 more
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Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts
BMC Molecular and Cell Biology, 2020 Background Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than ...
Iqbal Hyder +2 more
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Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation
Sensors, 2022 A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment.
Guilherme B. Pintarelli +3 more
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