Results 11 to 20 of about 135,255 (300)

NCLX Inhibition plus Ca²⁺ Delivery Potentiates Electroporation and Antitumor Immunity in Pancreatic Ductal Adenocarcinoma. [PDF]

Adv Sci (Weinh)
Electroporation combined with NCLX inhibition and Ca2+ delivery induces mitochondrial Ca2+ overload in PDAC cells, triggering pyroptosis, DAMP release, and antitumor immune activation. Simultaneously, suppression of tumor‐derived SPP1 weakens SPP1–CD44 signaling and remodels macrophages toward an antitumor phenotype, thereby enhancing CD8+ T‐cell ...
Zhao C, He L, Lu Y, Deng Z, Wei H, Zhang Y, Yang M, Yin T.   +7 more
europepmc   +2 more sources

Electric field effects of pulsed field ablation through adjacent non-cardiac tissues in an in vitro hybrid model [PDF]

Frontiers in Cardiovascular Medicine
BackgroundEndocardial ablation of atrial arrhythmias may occasionally produce insufficient lesion depth. Pulsed-field ablation (PFA) may generate measurable electroporation effects beyond interposed non-cardiac tissues, but field propagation across such ...
Denis Fedorov, Laura Dippel, Jan Minners, Johannes Steinfurt, Julian Mueller, Dirk Westermann, Heiko Lehrmann, Thomas Arentz, Martin Eichenlaub   +8 more
doaj   +2 more sources

Electroporation [PDF]

Cold Spring Harbor Protocols, 2019
Electroporation is a process in which brief electrical pulses create transient pores in the plasma membrane that allow nucleic acids to enter the cellular cytoplasm. Here, we provide information on the history, mechanism, and optimization of electroporation.
Priti, Kumar, Arvindhan, Nagarajan, Pradeep D, Uchil   +2 more
  +6 more sources

Controlled release of optimized electroporation enhances the transdermal efficiency of sinomenine hydrochloride for treating arthritis in vitro and in clinic

Drug Design, Development and Therapy, 2017
Shun Feng,1,* Lijun Zhu,1,* Zhisheng Huang,2 Haojia Wang,1 Hong Li,1 Hua Zhou,3 Linlin Lu,1 Ying Wang,1 Zhongqiu Liu,1,3 Liang Liu1,3 1International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou ...
Feng S, Zhu L, Huang Z, Wang H, Li H, Zhou H, Lu L, Wang Y, Liu Z, Liu L   +9 more
doaj   +1 more source

Electroporation of Mycobacteria [PDF]

Journal of Visualized Experiments, 2008
High efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall, but is compounded by the fact that most molecular techniques have been developed for distantly-related species such as Escherichia coli and Bacillus subtilis. In
Renan, Goude, Tanya, Parish
openaire   +3 more sources

Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

Micromachines, 2020
The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics ...
Yifei Ye, Xiaofeng Luan, Lingqian Zhang, Wenjie Zhao, Jie Cheng, Mingxiao Li, Yang Zhao, Chengjun Huang   +7 more
doaj   +1 more source

A transportable, inexpensive electroporator for in utero electroporation [PDF]

Development, Growth & Differentiation, 2015
Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude.
Torsten, Bullmann, Thomas, Arendt, Urs, Frey, Carina, Hanashima   +3 more
openaire   +2 more sources

Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

BMC Molecular and Cell Biology, 2020
Background Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than ...
Iqbal Hyder, Shahin Eghbalsaied, Wilfried A. Kues   +2 more
doaj   +1 more source

The Ins and Outs of Messenger RNA Electroporation for Physical Gene Delivery in Immune Cell-Based Therapy

Pharmaceutics, 2021
Messenger RNA (mRNA) electroporation is a powerful tool for transient genetic modification of cells. This non-viral method of genetic engineering has been widely used in immunotherapy. Electroporation allows fine-tuning of transfection protocols for each
Diana Campillo-Davo, Maxime De Laere, Gils Roex, Maarten Versteven, Donovan Flumens, Zwi N. Berneman, Viggo F. I. Van Tendeloo, Sébastien Anguille, Eva Lion   +8 more
doaj   +1 more source

Recent electroporation-based systems for intracellular molecule delivery

Nanotechnology and Precision Engineering, 2021
Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine.
Zaizai Dong, Lingqian Chang
doaj   +1 more source