Results 11 to 20 of about 112,914 (256)
Electroporation of Mycobacteria [PDF]
Journal of Visualized Experiments, 2008 High efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall, but is compounded by the fact that most molecular techniques have been developed for distantly-related species such as Escherichia coli and Bacillus subtilis. In
Renan, Goude, Tanya, Parish
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Effect of calcium electroporation in combination with metformin in vivo and correlation between viability and intracellular ATP level after calcium electroporation in vitro. [PDF]
PLoS ONE, 2017 BACKGROUND:Calcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses.
Stine Krog Frandsen, Julie Gehl
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APL Bioengineering, 2023 Electroporation shows great potential in biology and biomedical applications. However, there is still a lack of reliable protocol for cell electroporation to achieve a high perforation efficiency due to the unclear influence mechanism of various factors,
Qiongyao Mou +4 more
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Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation
Sensors, 2022 A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment.
Guilherme B. Pintarelli +3 more
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A transportable, inexpensive electroporator for in utero electroporation [PDF]
Development, Growth & Differentiation, 2015 Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude.
Torsten, Bullmann +3 more
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Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel
Micromachines, 2020 The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics ...
Yifei Ye +7 more
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Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts
BMC Molecular and Cell Biology, 2020 Background Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than ...
Iqbal Hyder +2 more
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The Journal of Reproduction and Development, 2022 Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing ...
Yui WAKE +3 more
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Recent electroporation-based systems for intracellular molecule delivery
Nanotechnology and Precision Engineering, 2021 Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine.
Zaizai Dong, Lingqian Chang
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Synergistic Combination of Electrolysis and Electroporation for Tissue Ablation. [PDF]
PLoS ONE, 2016 Electrolysis, electrochemotherapy with reversible electroporation, nanosecond pulsed electric fields and irreversible electroporation are valuable non-thermal electricity based tissue ablation technologies. This paper reports results from the first large
Michael K Stehling +5 more
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