Results 21 to 30 of about 112,914 (256)

Characterization of Experimentally Observed Complex Interplay between Pulse Duration, Electrical Field Strength, and Cell Orientation on Electroporation Outcome Using a Time-Dependent Nonlinear Numerical Model

open access: yesBiomolecules, 2023
Electroporation is a biophysical phenomenon involving an increase in cell membrane permeability to molecules after a high-pulsed electric field is applied to the tissue.
Maria Scuderi   +8 more
doaj   +1 more source

Irreversible Electroporation [PDF]

open access: yesSeminars in Interventional Radiology, 2015
Irreversible electroporation (IRE) is a predominantly nonthermal ablative technology that uses high-voltage, low-energy DC current pulses to induce cell death. Thermal ablative technologies such as radiofrequency ablation, microwave ablation, and cryoablation have several applications in oncology but have limitations that have been established. IRE has
openaire   +2 more sources

Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

open access: yesBMC Biotechnology, 2023
Background Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered.
Weiwei Liu   +7 more
doaj   +1 more source

Transfection by Electroporation [PDF]

open access: yesCurrent Protocols in Cell Biology, 1992
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
openaire   +5 more sources

Overview of Electroporation [PDF]

open access: yesTechnology in Cancer Research & Treatment, 2002
This special issue of Technology in Cancer Research and Treatment focuses on the versatility of electroporation. Contributed papers report on the basic mechanisms as well as the use of this procedure ex vivo and in vivo. In addition, recent studies utilizing in vivo electroporation for plasmid DNA and drug delivery are presented.
openaire   +2 more sources

Analysis of the Electric Field-Dependent Current During Electroporation Pulses

open access: yesIEEE Access, 2020
When delivered across a cell, certain pulsed electric fields can cause an increase in the cell membrane permeability through a biophysical process known as electroporation.
Yanpeng Lv   +6 more
doaj   +1 more source

Electroporation in Microfluidic Devices [PDF]

open access: yes, 2016
Crossing the plasma cellular membrane for loading of exogenous substances or accessing the intracellular medium is essential for cell engineering and transfection, cell analysis, or controlled extraction of the cellular content. Various chemical and physical techniques have been developed to open up the cell membrane and allow molecular exchange ...
Le Gac, Séverine, van Uitert, Iris
openaire   +3 more sources

Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro

open access: yesMolecules, 2021
Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses.
Tina Batista Napotnik   +1 more
doaj   +1 more source

Electroporation of cell membranes [PDF]

open access: yesBiophysical Journal, 1989
Electric pulses of intensity in kilovolts per centimeter and of duration in microseconds to milliseconds cause a temporary loss of the semipermeability of cell membranes, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
openaire   +2 more sources

The Effect of Conductivity Changes on Temperature Rise During Irreversible Electroporation

open access: yesFrontiers in Biomedical Technologies, 2020
Purpose: Irreversible electroporation is a physical process which is used for killing the cancer cells. The process that leads to cell death in this method is a unique process. Thermal damage does not exist in this process.
Amir Khorasani
doaj   +1 more source

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