Results 21 to 30 of about 133,618 (201)

Recent electroporation-based systems for intracellular molecule delivery

Nanotechnology and Precision Engineering, 2021
Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine.
Zaizai Dong, Lingqian Chang
doaj   +1 more source

Membrane electroporation and direct gene transfer [PDF]

, 1992
Neumann E. Membrane electroporation and direct gene transfer. Bioelectrochemistry and Bioenergetics. 1992;28(1-2):247-267.The direct transfer of genetic material into cells by electroporation can be described in physicochemical terms as an ...
Eberhard Neumann, Neumann, Eberhard
core   +1 more source

Modelling single cell electroporation with bipolar pulse parameters and dynamic pore radii [PDF]

, 2010
We develop a model of single spherical cell electroporation and simulate spatial and temporal aspects of the transmembrane potential and pore radii as an effect of any form of applied electric field.
van Ekeran, Jethro, Talele, Sadhana, Gaynor, Paul, Cree, Michael J.   +3 more
core   +1 more source

Model of cell electrofusion Membrane electroporation, pore coalescence and percolation [PDF]

, 1987
Sugar IP, Förster W, Neumann E. Model of cell electrofusion Membrane electroporation, pore coalescence and percolation. Biophysical Chemistry.
Förster, Walter, Istvan P. Sugar, Eberhard Neumann, Sugar, Istvan P., Walter Förster, Neumann, Eberhard   +5 more
core   +1 more source

Characterization of Experimentally Observed Complex Interplay between Pulse Duration, Electrical Field Strength, and Cell Orientation on Electroporation Outcome Using a Time-Dependent Nonlinear Numerical Model

Biomolecules, 2023
Electroporation is a biophysical phenomenon involving an increase in cell membrane permeability to molecules after a high-pulsed electric field is applied to the tissue.
Maria Scuderi, Janja Dermol-Černe, Tina Batista Napotnik, Sebastien Chaigne, Olivier Bernus, David Benoist, Daniel C. Sigg, Lea Rems, Damijan Miklavčič   +8 more
doaj   +1 more source

Synergistic Combination of Electrolysis and Electroporation for Tissue Ablation. [PDF]

PLoS ONE, 2016
Electrolysis, electrochemotherapy with reversible electroporation, nanosecond pulsed electric fields and irreversible electroporation are valuable non-thermal electricity based tissue ablation technologies. This paper reports results from the first large
Michael K Stehling, Enric Guenther, Paul Mikus, Nina Klein, Liel Rubinsky, Boris Rubinsky   +5 more
doaj   +1 more source

Analysis of the Electric Field-Dependent Current During Electroporation Pulses

IEEE Access, 2020
When delivered across a cell, certain pulsed electric fields can cause an increase in the cell membrane permeability through a biophysical process known as electroporation.
Yanpeng Lv, Xian Cheng, Shuo Chen, Hongmei Liu, Yilin Wang, Chenguo Yao, Boris Rubinsky   +6 more
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Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system

BMC Biotechnology, 2023
Background Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered.
Weiwei Liu, Xiaoguo Wang, Ruirong Liu, Yaya Liao, Zhiwei Peng, Haoyun Jiang, Qiqi Jing, Yuyun Xing   +7 more
doaj   +1 more source

Transfection by Electroporation [PDF]

Current Protocols in Cell Biology, 1992
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
openaire   +5 more sources

Overview of Electroporation [PDF]

Technology in Cancer Research & Treatment, 2002
This special issue of Technology in Cancer Research and Treatment focuses on the versatility of electroporation. Contributed papers report on the basic mechanisms as well as the use of this procedure ex vivo and in vivo. In addition, recent studies utilizing in vivo electroporation for plasmid DNA and drug delivery are presented.
openaire   +2 more sources