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Electroporation is a biophysical phenomenon involving an increase in cell membrane permeability to molecules after a high-pulsed electric field is applied to the tissue.
Maria Scuderi +8 more
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Irreversible Electroporation [PDF]
Irreversible electroporation (IRE) is a predominantly nonthermal ablative technology that uses high-voltage, low-energy DC current pulses to induce cell death. Thermal ablative technologies such as radiofrequency ablation, microwave ablation, and cryoablation have several applications in oncology but have limitations that have been established. IRE has
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Background Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to be delivered.
Weiwei Liu +7 more
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Transfection by Electroporation [PDF]
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
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Overview of Electroporation [PDF]
This special issue of Technology in Cancer Research and Treatment focuses on the versatility of electroporation. Contributed papers report on the basic mechanisms as well as the use of this procedure ex vivo and in vivo. In addition, recent studies utilizing in vivo electroporation for plasmid DNA and drug delivery are presented.
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Analysis of the Electric Field-Dependent Current During Electroporation Pulses
When delivered across a cell, certain pulsed electric fields can cause an increase in the cell membrane permeability through a biophysical process known as electroporation.
Yanpeng Lv +6 more
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Electroporation in Microfluidic Devices [PDF]
Crossing the plasma cellular membrane for loading of exogenous substances or accessing the intracellular medium is essential for cell engineering and transfection, cell analysis, or controlled extraction of the cellular content. Various chemical and physical techniques have been developed to open up the cell membrane and allow molecular exchange ...
Le Gac, Séverine, van Uitert, Iris
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Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses.
Tina Batista Napotnik +1 more
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Electroporation of cell membranes [PDF]
Electric pulses of intensity in kilovolts per centimeter and of duration in microseconds to milliseconds cause a temporary loss of the semipermeability of cell membranes, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
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The Effect of Conductivity Changes on Temperature Rise During Irreversible Electroporation
Purpose: Irreversible electroporation is a physical process which is used for killing the cancer cells. The process that leads to cell death in this method is a unique process. Thermal damage does not exist in this process.
Amir Khorasani
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