Results 21 to 30 of about 182,543 (292)

Effect of calcium electroporation in combination with metformin in vivo and correlation between viability and intracellular ATP level after calcium electroporation in vitro. [PDF]

open access: yesPLoS ONE, 2017
BACKGROUND:Calcium electroporation is a new experimental anti-cancer treatment where calcium is internalized into cells by application of short, high voltage pulses.
Stine Krog Frandsen, Julie Gehl
doaj   +1 more source

Accounting Problems Under the Excess Profits Tax [PDF]

open access: yes, 1943
DNA vaccines based on subunits from pathogens have several advantages over other vaccine strategies. DNA vaccines can easily be modified, they show good safety profiles, are stable and inexpensive to produce, and the immune response can be focused to the
A Biragyn   +73 more
core   +9 more sources

Studying the roles of salt ions in the pore initiation and closure stages in the biomembrane electroporation

open access: yesAPL Bioengineering, 2023
Electroporation shows great potential in biology and biomedical applications. However, there is still a lack of reliable protocol for cell electroporation to achieve a high perforation efficiency due to the unclear influence mechanism of various factors,
Qiongyao Mou   +4 more
doaj   +1 more source

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

open access: yesSensors, 2022
A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment.
Guilherme B. Pintarelli   +3 more
doaj   +1 more source

Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

open access: yesMicromachines, 2020
The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics ...
Yifei Ye   +7 more
doaj   +1 more source

Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

open access: yesBMC Molecular and Cell Biology, 2020
Background Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than ...
Iqbal Hyder   +2 more
doaj   +1 more source

Recent electroporation-based systems for intracellular molecule delivery

open access: yesNanotechnology and Precision Engineering, 2021
Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an important method for investigating cellular mechanisms and changing cell fates in biomedicine.
Zaizai Dong, Lingqian Chang
doaj   +1 more source

Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate

open access: yesThe Journal of Reproduction and Development, 2022
Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing ...
Yui WAKE   +3 more
doaj   +1 more source

Irreversible Electroporation [PDF]

open access: yesSeminars in Interventional Radiology, 2015
Irreversible electroporation (IRE) is a predominantly nonthermal ablative technology that uses high-voltage, low-energy DC current pulses to induce cell death. Thermal ablative technologies such as radiofrequency ablation, microwave ablation, and cryoablation have several applications in oncology but have limitations that have been established. IRE has
openaire   +2 more sources

Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene Therapy Model [PDF]

open access: yes, 2008
Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model.
C. H. Hsu   +4 more
core   +2 more sources

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