Results 21 to 30 of about 595,494 (264)
Transfection by Electroporation [PDF]
Current Protocols in Cell Biology, 1992 AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
openaire +5 more sources
Nanomaterials, 2022 Many polymeric gene delivery nano-vectors with hyperbranched structures have been demonstrated to be superior to their linear counterparts. The higher delivery efficacy is commonly attributed to the abundant terminal groups of branched polymers, which ...
Yinghao Li +11 more
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Magnetochemistry, 2023 In recent years, nanotechnology has deployed a new set of theragnostic tools, including magnetic resonance contrast agents, nano-delivery systems and magnetic hyperthermia treatments in cancer therapy, exploiting not only the small size of nanoparticles,
Manuel A. González-Gómez +6 more
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Lipid droplet targeting domains of adipophilin
Journal of Lipid Research, 2003 Adipophilin (ADPH), a prominent protein component of lipid storage droplets (LSDs), is postulated to be necessary for the formation and cellular function of these structures.
James L. McManaman +3 more
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Efficient Delivery of SARS-CoV-2 Plasmid DNA in HEK-293T Cells Using Chitosan Nanoparticles
PharmaceuticalsBackground/Objectives: Gene therapy has emerged as a promising strategy for treating a wide range of diseases. However, a major challenge remains in developing efficient and safe delivery systems for genetic material. Nanoparticles, particularly chitosan
Citlali Cecilia Mendoza-Guevara +4 more
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Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Journal of Venomous Animals and Toxins including Tropical Diseases, 2007 A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested.
P. Carnieli JR +2 more
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Evaluation of Lipid-Based Transfection in Primary Monocytes Within an Ex Vivo Whole-Blood Model
BiomoleculesTransfection is a fundamental method in biomedical research to study intracellular molecular mechanisms by manipulating target protein expression. Various methods have been developed to deliver nucleic acids into the cells of interest in vitro, with ...
Robin Moolan-Vadackumchery +2 more
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An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
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Frontiers in Bioengineering and BiotechnologyBackgroundHuman bone marrow mesenchymal stem cell (MSC) administration reduces inflammation in pre-clinical models of sepsis and sepsis-related lung injury, however clinical efficacy in patients has not yet been demonstrated.
Rahul Y. Mahida +9 more
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Frontiers in Microbiology, 2020 Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from Sclerotinia sclerotiorum. This virus, named S.
Qihua Wang +10 more
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