Results 21 to 30 of about 686,707 (295)

Transfection by Electroporation [PDF]

Current Protocols in Cell Biology, 1992
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
openaire   +5 more sources

Transfection of Platyhelminthes [PDF]

BioMed Research International, 2015
Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans.
Moguel, Bárbara, Bobes, Raúl J., Carrero, Julio C., Laclette, Juan P.   +3 more
openaire   +2 more sources

Optical sorting and photo-transfection of mammalian cells [PDF]

, 2010
Recently, laser light sources of different regimes have emerged as an essential tool in the biophotonics research area. Classic applications include, for example: manipulating single cells and their subcellular organelles, sorting cells in microfluidic ...
Mthunzi, Patience
core   +2 more sources

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system

Royal Society Open Science, 2022
Microfluidic tools have recently made possible many advances in biological and biomedical research. Research in fields such as physics, engineering, chemistry and biology have combined to produce innovation in microfluidics which has positively impacted ...
Camilo Pérez-Sosa, Anahí Sanluis-Verdes, Ariel Waisman, Antonella Lombardi, Gustavo Rosero, Alejandro La Greca, Shekhar Bhansali, Natalia Bourguignon, Carlos Luzzani, Maximiliano. S. Pérez, Santiago Miriuka, Betiana Lerner   +11 more
doaj   +1 more source

Green Fluorescent Protein is a Suitable Reporter of Tumor Hypoxia Despite an Oxygen Requirement for Chromophore Formation

Neoplasia: An International Journal for Oncology Research, 2001
The oxygen requirement for chromophore formation potentially limits the use of green fluorescent protein as a reporter under hypoxic conditions. In the light of this, the applicability of a hypoxia-responsive enhanced green fluorescent protein (EGFP ...
Dirk Vordermark, Toru Shibata, J. Martin Brown   +2 more
doaj   +1 more source

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Cells, 2020
In the last few decades, stem cell therapy has grown as a boon for many pathological complications including female reproductive disorders. In this review, a brief description of available strategies that are related to stem cell-based in vitro oocyte ...
Dinesh Bharti, Si-Jung Jang, Sang-Yun Lee, Sung-Lim Lee, Gyu-Jin Rho   +4 more
doaj   +1 more source

G-Quadruplex Linked DNA Guides Selective Transfection into Nucleolin-Overexpressing Cancer Cells

Pharmaceutics, 2022
Gene therapy is a promising approach for treating tumors. Conventional approaches of DNA delivery depending on non-viral or viral vectors are unsatisfactory due to the concerns of biosafety and cell-targeting efficiency.
Mengxi Xiang, Yongkui Li, Jia Liu, Jie Shi, Yizhi Ge, Chen Peng, Yawen Bin, Zheng Wang, Lin Wang   +8 more
doaj   +1 more source

Hyperbranched Poly(β-amino ester)s (HPAEs) Structure Optimisation for Enhanced Gene Delivery: Non-Ideal Termination Elimination

Nanomaterials, 2022
Many polymeric gene delivery nano-vectors with hyperbranched structures have been demonstrated to be superior to their linear counterparts. The higher delivery efficacy is commonly attributed to the abundant terminal groups of branched polymers, which ...
Yinghao Li, Zhonglei He, Jing Lyu, Xianqing Wang, Bei Qiu, Irene Lara-Sáez, Jing Zhang, Ming Zeng, Qian Xu, Sigen A, James F. Curtin, Wenxin Wang   +11 more
doaj   +1 more source

Fluorescent Magnetic Mesoporous Nanoprobes for Biotechnological Enhancement Procedures in Gene Therapy

Magnetochemistry, 2023
In recent years, nanotechnology has deployed a new set of theragnostic tools, including magnetic resonance contrast agents, nano-delivery systems and magnetic hyperthermia treatments in cancer therapy, exploiting not only the small size of nanoparticles,
Manuel A. González-Gómez, Román Seco-Gudiña, Pelayo García-Acevedo, Ángela Arnosa-Prieto, Lisandra de Castro-Alves, Yolanda Piñeiro, José Rivas   +6 more
doaj   +1 more source