Results 21 to 30 of about 733,470 (308)
Marine Drugs, 2023 The determination of the protein’s intracellular localization is essential for understanding its biological function. Protein localization studies are mainly performed on primary and secondary vertebrate cell lines for which most protocols have been ...
Kristina Dominko +6 more
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Generating Recombinant Antibodies to Membrane Proteins through Phage Display
Antibodies, 2016 One of the most important classes of proteins in terms of drug targets is cell surface membrane proteins, and yet it is a challenging set of proteins for generating high-quality affinity reagents.
Renhua Huang +4 more
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Вавиловский журнал генетики и селекции, 2019 The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova +11 more
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Transfection by Electroporation [PDF]
Current Protocols in Cell Biology, 1992 AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
openaire +5 more sources
Nature Communications, 2018 Effective transfection of genetic molecules such as DNA usually relies on vectors that can reversibly uptake and release these molecules, and protect them from digestion by nuclease.
Shuang Peng +9 more
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Royal Society Open Science, 2022 Microfluidic tools have recently made possible many advances in biological and biomedical research. Research in fields such as physics, engineering, chemistry and biology have combined to produce innovation in microfluidics which has positively impacted ...
Camilo Pérez-Sosa +11 more
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Nature Communications, 2019 Current therapies for most neurodegenerative disorders are only symptomatic in nature and do not change the course of the disease. Gene therapy plays an important role in disease modifying therapeutic strategies.
Shuai Liu +11 more
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Neoplasia: An International Journal for Oncology Research, 2001 The oxygen requirement for chromophore formation potentially limits the use of green fluorescent protein as a reporter under hypoxic conditions. In the light of this, the applicability of a hypoxia-responsive enhanced green fluorescent protein (EGFP ...
Dirk Vordermark +2 more
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In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded
Cells, 2020 In the last few decades, stem cell therapy has grown as a boon for many pathological complications including female reproductive disorders. In this review, a brief description of available strategies that are related to stem cell-based in vitro oocyte ...
Dinesh Bharti +4 more
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G-Quadruplex Linked DNA Guides Selective Transfection into Nucleolin-Overexpressing Cancer Cells
Pharmaceutics, 2022 Gene therapy is a promising approach for treating tumors. Conventional approaches of DNA delivery depending on non-viral or viral vectors are unsatisfactory due to the concerns of biosafety and cell-targeting efficiency.
Mengxi Xiang +8 more
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