Results 21 to 30 of about 692,115 (252)

Targeted genome modifcation in protoplasts of a highly regenerable Siberian barley cultivar using RNA-guided Cas9 endonuclease

Вавиловский журнал генетики и селекции, 2019
The modifcation of crop genomes employing functional components of the microbial CRISPR/Cas immune system is a rapidly developing area of applied research.
S. V. Gerasimova, A. M. Korotkova, C. Hertig, S. Hiekel, R. Hofe, N. Budhagatapalli, I. Otto, G. Hensel, V. K. Shumny, A. V. Kochetov, J. Kumlehn, E. K. Khlestkina   +11 more
doaj   +1 more source

Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system

Royal Society Open Science, 2022
Microfluidic tools have recently made possible many advances in biological and biomedical research. Research in fields such as physics, engineering, chemistry and biology have combined to produce innovation in microfluidics which has positively impacted ...
Camilo Pérez-Sosa, Anahí Sanluis-Verdes, Ariel Waisman, Antonella Lombardi, Gustavo Rosero, Alejandro La Greca, Shekhar Bhansali, Natalia Bourguignon, Carlos Luzzani, Maximiliano. S. Pérez, Santiago Miriuka, Betiana Lerner   +11 more
doaj   +1 more source

In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded

Cells, 2020
In the last few decades, stem cell therapy has grown as a boon for many pathological complications including female reproductive disorders. In this review, a brief description of available strategies that are related to stem cell-based in vitro oocyte ...
Dinesh Bharti, Si-Jung Jang, Sang-Yun Lee, Sung-Lim Lee, Gyu-Jin Rho   +4 more
doaj   +1 more source

Green Fluorescent Protein is a Suitable Reporter of Tumor Hypoxia Despite an Oxygen Requirement for Chromophore Formation

Neoplasia: An International Journal for Oncology Research, 2001
The oxygen requirement for chromophore formation potentially limits the use of green fluorescent protein as a reporter under hypoxic conditions. In the light of this, the applicability of a hypoxia-responsive enhanced green fluorescent protein (EGFP ...
Dirk Vordermark, Toru Shibata, J. Martin Brown   +2 more
doaj   +1 more source

G-Quadruplex Linked DNA Guides Selective Transfection into Nucleolin-Overexpressing Cancer Cells

Pharmaceutics, 2022
Gene therapy is a promising approach for treating tumors. Conventional approaches of DNA delivery depending on non-viral or viral vectors are unsatisfactory due to the concerns of biosafety and cell-targeting efficiency.
Mengxi Xiang, Yongkui Li, Jia Liu, Jie Shi, Yizhi Ge, Chen Peng, Yawen Bin, Zheng Wang, Lin Wang   +8 more
doaj   +1 more source

Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells [PDF]

, 2015
Kunwoo Lee,1,2 Pengzhi Yu,3 Nithya Lingampalli,1 Hyun Jin Kim,1 Richard Tang,1 Niren Murthy1,2 1Department of Bioengineering, University of California, Berkeley, CA, USA; 2UC Berkeley and UCSF Joint Graduate Program in Bioengineering, Berkeley/San ...
Lingampalli N, Yu P, Kim HJ, Murthy N, Lee K, Tang R   +5 more
core   +2 more sources

Hyperbranched Poly(β-amino ester)s (HPAEs) Structure Optimisation for Enhanced Gene Delivery: Non-Ideal Termination Elimination

Nanomaterials, 2022
Many polymeric gene delivery nano-vectors with hyperbranched structures have been demonstrated to be superior to their linear counterparts. The higher delivery efficacy is commonly attributed to the abundant terminal groups of branched polymers, which ...
Yinghao Li, Zhonglei He, Jing Lyu, Xianqing Wang, Bei Qiu, Irene Lara-Sáez, Jing Zhang, Ming Zeng, Qian Xu, Sigen A, James F. Curtin, Wenxin Wang   +11 more
doaj   +1 more source

Fluorescent Magnetic Mesoporous Nanoprobes for Biotechnological Enhancement Procedures in Gene Therapy

Magnetochemistry, 2023
In recent years, nanotechnology has deployed a new set of theragnostic tools, including magnetic resonance contrast agents, nano-delivery systems and magnetic hyperthermia treatments in cancer therapy, exploiting not only the small size of nanoparticles,
Manuel A. González-Gómez, Román Seco-Gudiña, Pelayo García-Acevedo, Ángela Arnosa-Prieto, Lisandra de Castro-Alves, Yolanda Piñeiro, José Rivas   +6 more
doaj   +1 more source

Lipid droplet targeting domains of adipophilin

Journal of Lipid Research, 2003
Adipophilin (ADPH), a prominent protein component of lipid storage droplets (LSDs), is postulated to be necessary for the formation and cellular function of these structures.
James L. McManaman, William Zabaronick, Jerome Schaack, David J. Orlicky   +3 more
doaj   +1 more source

Efficient Delivery of SARS-CoV-2 Plasmid DNA in HEK-293T Cells Using Chitosan Nanoparticles

Pharmaceuticals
Background/Objectives: Gene therapy has emerged as a promising strategy for treating a wide range of diseases. However, a major challenge remains in developing efficient and safe delivery systems for genetic material. Nanoparticles, particularly chitosan
Citlali Cecilia Mendoza-Guevara, Alejandro Martinez-Escobar, María del Pilar Ramos-Godínez, José Esteban Muñoz-Medina, Eva Ramon-Gallegos   +4 more
doaj   +1 more source