Quantification Bias Caused by Plasmid DNA Conformation in Quantitative Real-Time PCR Assay [PDF]
PLoS ONE, 2011 Quantitative real-time PCR (qPCR) is the gold standard for the quantification of specific nucleic acid sequences. However, a serious concern has been revealed in a recent report: supercoiled plasmid standards cause significant over-estimation in qPCR ...
Tzu-Ming Pan, Yu-Chieh Chen
exaly +3 more sources
A Comparison of Plasmid DNA and mRNA as Vaccine Technologies
Vaccines, 2019 This review provides a comparison of the theoretical issues and experimental findings for plasmid DNA and mRNA vaccine technologies. While both have been under development since the 1990s, in recent years, significant excitement has turned to mRNA ...
Margaret A Liu
exaly +2 more sources
Induction of translation-suppressive G3BP1+ stress granules and interferon-signaling cGAS condensates by transfected plasmid DNA [PDF]
hLifePlasmid DNA transfection is one of the fundamental tools of biomedical research. Here, we found that plasmid DNA transfection mediated by liposomes activates multiple innate immune responses in several widely used cell lines.
Vladimir Majerciak, Zhi-Ming Zheng
doaj +2 more sources
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Nucleic Acids Research, 1979 Hyman C Birnboim, J Doly
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Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer
Gene Therapy, 1999 Martin Haardt +2 more
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A new device-mediated miniprep method
AMB Express, 2022 Keypoints New devise-mediated plasmid miniprep method (DM) performs all mixing steps without operator intervention. The DM method produces plasmid DNAs free of the dCCC form and significantly reduces the contamination with genomic DNA in the low-copy ...
Baryshev Mikhail +2 more
doaj +1 more source
Separation Properties of Plasmid DNA Using a Two-Stage Particle Adsorption-Microfiltration Process
Membranes, 2023 Plasmid DNA is used as a vector for gene therapy and DNA vaccination; therefore, the establishment of a mass production method is required. Membrane filtration is widely employed as a separation method suitable for the mass production of plasmid DNA ...
Nobuyuki Katagiri +4 more
doaj +1 more source
Plasmid DNA for Therapeutic Applications in Cancer
Pharmaceutics, 2022 Recently, the interest in using nucleic acids for therapeutic applications has been increasing. DNA molecules can be manipulated to express a gene of interest for gene therapy applications or vaccine development.
D. H. Martínez-Puente +9 more
semanticscholar +1 more source
Effects of Argonaute on Gene Expression in Thermus thermophilus. [PDF]
PLoS ONE, 2015 Eukaryotic Argonaute proteins mediate RNA-guided RNA interference, allowing both regulation of host gene expression and defense against invading mobile genetic elements.
Daan C Swarts +4 more
doaj +1 more source
Application of gene therapy in the treatment of superficial digital flexor tendon injury in horses [PDF]
Open Veterinary Journal, 2020 Background: Tendon injuries are one of the most common causes of orthopedic disorders in horses. Such injuries involve a long course of treatment and recovery.
Alexandr Aimaletdinov +8 more
doaj +1 more source