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Topological Behavior of Plasmid DNA [PDF]

open access: yesMicrobiology Spectrum, 2014
ABSTRACT The discovery of the B-form structure of DNA by Watson and Crick led to an explosion of research on nucleic acids in the fields of biochemistry, biophysics, and genetics. Powerful techniques were developed to reveal a myriad of different structural conformations that change B-DNA as it is transcribed, replicated, and ...
N Patrick, Higgins   +1 more
openaire   +2 more sources

Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems [PDF]

open access: yes, 2013
Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo ...
Redzej, Adam   +7 more
core   +1 more source

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR. [PDF]

open access: yesPLoS ONE, 2017
Bacteria can be considered as biological nanofactories that manufacture a cornucopia of bioproducts most notably recombinant proteins. As such, they must perfectly match with appropriate plasmid vectors to ensure successful overexpression of target genes.
Magdalena Plotka   +2 more
doaj   +1 more source

Bacteria spring a surprise [PDF]

open access: yes, 2014
Cell division involves the precise duplication of all the genetic material in the cell—both the chromosomes and the plasmid DNA—and the equal division of this material between the two daughter cells.
Srinivasan, Rajagopalan   +3 more
core   +1 more source

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR [PDF]

open access: yes, 2015
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories.
Mitterbauer, G.   +316 more
core   +3 more sources

Linear DNA plasmids of yeasts [PDF]

open access: yesFEMS Microbiology Letters, 1995
Linear DNA plasmids are common among filamentous fungi and plants in which they are generally associated with mitochondria. In contrast, the linear DNA plasmids of yeast appear to be cytoplasmic and to have a specific genetic organization. In these respects the yeast linear plasmids can be regarded as a separate group of plasmids.
openaire   +2 more sources

Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene. [PDF]

open access: yesPLoS ONE, 2010
Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard.
Yubo Hou   +3 more
doaj   +1 more source

Immunogenicity of COVID-eVax Delivered by Electroporation Is Moderately Impacted by Temperature and Molecular Isoforms

open access: yesVaccines, 2023
DNA integrity is a key issue in gene therapy and genetic vaccine approaches based on plasmid DNA. In contrast to messenger RNA that requires a controlled cold chain for efficacy, DNA molecules are considered to be more stable.
Federico D’Alessio   +11 more
doaj   +1 more source

Carbon-nanotube for Transient Expression in Rice Calli

open access: yesHayati Journal of Biosciences, 2022
Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency.
Paphawarin Pinyokham   +5 more
doaj   +1 more source

Optimising DNA binding to carbon nanotubes by non-covalent methods [PDF]

open access: yes, 2011
The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors.
Coley, Helen M.   +23 more
core   +1 more source

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