Results 51 to 60 of about 2,233,350 (305)
Computing with DNA by operating on plasmids
Biosystems, 2000 A new method of computing using DNA plasmids is introduced and the potential advantages are listed. The new method is illustrated by reporting a laboratory computation of an instance of the NP-complete algorithmic problem of computing the cardinal number of a maximal independent subset of the vertex set of a graph.
Head, T. +5 more
openaire +3 more sources
BMC Biotechnology, 2003 Background Non-viral vectors for gene transfer are less immunogenic than viral vectors but also less efficient. Significant effort has focused on enhancing non-viral gene transfer efficiency by increasing nuclear import of plasmid DNA, particularly by ...
Escriou Virginie +3 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Verfahren zur Herstellung pharmazeutischer Plasmid-DNA [PDF]
, 2008 Voß C. Processes for the production of pharmaceutical grade plasmid DNA. Bielefeld (Germany): Bielefeld University; 2008.Plasmid DNA is currently used in gene therapy and genetic vaccination as a vector system for the delivery of therapeutic genes ...
Voß, Carsten
core
Methods and Protocols, 2020 In transfection experiments with mammalian cells aiming to overexpress a specific protein, it is often necessary to correctly quantify the level of the recombinant and the corresponding endogenous mRNA.
Barbara Ahlemeyer +2 more
doaj +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Biophysical analysis of diffusion controlled processes in the budding yeast nucleus [PDF]
, 2009 The spatial organization of chromosomes and the dynamics of chromosome reorganization have been shown to be crucial for the regulation of gene expression and various aspects of DNA metabolism.
Gehlen, Lutz Robert
core +1 more source
Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids [PDF]
, 2011 The extrachromosomal gene pool plays a significant role both in evolution and in the environmental adaptation of bacteria. The L. lactis subsp. lactis IL594 strain contains seven plasmids, named pIL1 to pIL7, and is the parental strain of the plasmid ...
Roman K Górecki +19 more
core +2 more sources
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Photoinduced cleavage of plasmid DNA in the presence of pterin
Pteridines, 2000 The photoinduced cleavage of plasmid DNA by UV -A light in the presence of pterin was investigated. Electrophoretic analysis of the irradiated plasmid pUCI8 in the presence of pterin showed that UV light of 350 nm induced the transformation of a ...
Lorente Carolina +5 more
doaj +1 more source