Results 31 to 40 of about 2,233,350 (305)

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia, Webb, Martin R, Mashanov, Gregory I, Chisty, Liisa T, Toseland, Christopher P., Dillingham, Mark S, Toseland, Christopher P, Molloy, Justin E   +7 more
core   +1 more source

A one-step miniprep for the isolation of plasmid DNA and lambda phage particles. [PDF]

PLoS ONE, 2011
Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format.
George Lezin, Yasuhiro Kosaka, H Joseph Yost, Michael R Kuehn, Luca Brunelli   +4 more
doaj   +1 more source

Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA [PDF]

, 2008
Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested.
Abee, T., Nierop Groot, M.N., M. N. Groot, Nieboer, F., F. Nieboer, T. Abee   +5 more
core   +1 more source

A New Technique With Calcium Phosphate Precipitate Enhances Efficiency of In Vivo Plasmid DNA Gene Transfer

Journal of Pharmacological Sciences, 2005
In vivo gene transfer with plasmid vector has been applied experimentally and clinically; however, the low level of gene transfer efficiency with plasmid vector is a problem.
Shinji Kuroda, Hisatomo Kondo, Keiichi Ohya, Shohei Kasugai   +3 more
doaj   +1 more source

Protocol for the High-quality Plasmid Isolation from Different Recalcitrant Bacterial Species: Agrobacterium spp., Rhizobium sp., and Bacillus thuringiensis

Bio-Protocol, 2023
High yield of good quality plasmid DNA from gram -ve bacteria (Agrobacterium tumefaciens, A. rhizogenes, and Rhizobium sp.) and gram +ve bacterium (Bacillus thuringiensis) is difficult. The widely used plasmid extraction kits for Escherichia coli yield a
Preshobha Kodackattumannil, Shina Sasi, Saranya Krishnan, Geetha Lekshmi, Martin Kottackal, Khaled Amiri   +5 more
doaj   +1 more source

Protective antiviral immune responses to pseudorabies virus induced by DNA vaccination using dimethyldioctadecylammonium bromide as an adjuvant [PDF]

, 2002
To enhance the efficacy of a DNA vaccine against pseudorabies virus (PRV), we evaluated the adjuvant properties of plasmids coding for gamma interferon or interleukin-12, of CpG immunostimulatory motifs, and of the conventional adjuvants ...
Eddie G. te Lintelo, te Lintelo, E.G., Wim J. A. Boersma, Rooij, E.M.A., van, Yolanda E. de Visser, Boersma, WJA, Lintelo, E.G., te, Boersma, W.J.A., Visser, Y.E., de, Hilgers, LAT, Haagmans, Bart, van Rooij, EMA, Bart L. Haagmans, Bianchi, ATJ, Harrie L. Glansbeek, Hilgers, L.A.T., Haagmans, B.L., Glansbeek, H.L., Andre T. J. Bianchi, van Rooij, E.M.A., Glansbeek, HL, Eugene M. A. van Rooij, de Visser, Y.E., Luuk A. T. Hilgers, Bianchi, A.T.J., Lintelo, EG, de Visser, YE   +26 more
core   +1 more source

Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

Synthetic and Systems Biotechnology, 2019
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories.
Lorna Suckling, Ciaran McFarlane, Chelsea Sawyer, Stephen P. Chambers, Richard I. Kitney, David W. McClymont, Paul S. Freemont   +6 more
doaj   +1 more source

Scientific advice on the suitability of data for the assessment of DNA integration into the fish genome of a genetically modified DNA plasmid-based veterinary vaccine [PDF]

EFSA Journal, 2013
Pancreas disease caused by salmonid alphavirus in farmed Atlantic salmon (Salmo salar) leads to high mortality rates post infection and histopathological lesions in several organs. As protection against pancreas disease, Novartis developed a prophylactic
European Food Safety Authority
doaj   +1 more source

Mathematical and experimental approaches to the dimer catastrophe theory [PDF]

, 2011
Multicopy plasmids rely on random distribution for stable inheritance by daughter cells at division. Threats to plasmid copy number increase the probability of plasmid loss, which can be detrimental to both plasmid and host. Plasmid dimers emerge through

core   +3 more sources

Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells. [PDF]

PLoS Genetics, 2009
Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA.
Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann   +7 more
doaj   +1 more source