Results 31 to 40 of about 2,326,635 (307)

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR [PDF]

open access: yes, 2014
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories.
Mitterbauer, G.   +539 more
core   +2 more sources

Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation

open access: yesSynthetic and Systems Biotechnology, 2019
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories.
Lorna Suckling   +6 more
doaj   +1 more source

Topological Behavior of Plasmid DNA [PDF]

open access: yesMicrobiology Spectrum, 2014
ABSTRACT The discovery of the B-form structure of DNA by Watson and Crick led to an explosion of research on nucleic acids in the fields of biochemistry, biophysics, and genetics. Powerful techniques were developed to reveal a myriad of different structural conformations that change B-DNA as it is transcribed, replicated, and ...
N Patrick, Higgins   +1 more
openaire   +2 more sources

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR. [PDF]

open access: yesPLoS ONE, 2017
Bacteria can be considered as biological nanofactories that manufacture a cornucopia of bioproducts most notably recombinant proteins. As such, they must perfectly match with appropriate plasmid vectors to ensure successful overexpression of target genes.
Magdalena Plotka   +2 more
doaj   +1 more source

Linear DNA plasmids of yeasts [PDF]

open access: yesFEMS Microbiology Letters, 1995
Linear DNA plasmids are common among filamentous fungi and plants in which they are generally associated with mitochondria. In contrast, the linear DNA plasmids of yeast appear to be cytoplasmic and to have a specific genetic organization. In these respects the yeast linear plasmids can be regarded as a separate group of plasmids.
openaire   +2 more sources

Determinants of selectivity in Xer site-specific recombination [PDF]

open access: yes, 1996
A remarkable property of some DNA-binding proteins that can interact with and pair distant DNA segments is that they mediate their biological function only when their binding sites are arranged in a specific configuration. Xer site-specific recombination
Blakely, G, Sherratt, D
core   +1 more source

Carbon-nanotube for Transient Expression in Rice Calli

open access: yesHayati Journal of Biosciences, 2022
Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency.
Paphawarin Pinyokham   +5 more
doaj   +1 more source

Characterization of Ku70(2)-NLS as bipartite nuclear localization sequence for non-viral gene delivery. [PDF]

open access: yes, 2012
Several barriers have to be overcome in order to achieve gene expression in target cells, e.g. cellular uptake, endosomal release and translocation to the nucleus.
Maucksch, Christof   +17 more
core   +2 more sources

Immunogenicity of COVID-eVax Delivered by Electroporation Is Moderately Impacted by Temperature and Molecular Isoforms

open access: yesVaccines, 2023
DNA integrity is a key issue in gene therapy and genetic vaccine approaches based on plasmid DNA. In contrast to messenger RNA that requires a controlled cold chain for efficacy, DNA molecules are considered to be more stable.
Federico D’Alessio   +11 more
doaj   +1 more source

Serious overestimation in quantitative PCR by circular (supercoiled) plasmid standard: microalgal pcna as the model gene. [PDF]

open access: yesPLoS ONE, 2010
Quantitative real-time PCR (qPCR) has become a gold standard for the quantification of nucleic acids and microorganism abundances, in which plasmid DNA carrying the target genes are most commonly used as the standard.
Yubo Hou   +3 more
doaj   +1 more source

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