Results 11 to 20 of about 3,138,280 (305)

The Miraprep: A Protocol that Uses a Miniprep Kit and Provides Maxiprep Yields. [PDF]

PLoS ONE, 2016
Plasmid purification is a basic tool of molecular biologists. Although the development of plasmid isolation kits utilizing silica spin columns reduced the time and labor spent on plasmid purification, achieving large plasmid DNA yields still requires ...
Mira I Pronobis, Natalie Deuitch, Mark Peifer   +2 more
doaj   +1 more source

Rapid method for plasmid DNA recombination (Murakami-system)

MethodsX, 2023
DNA recombination is a useful technology for cloning and subsequent functional analysis, while standard techniques for plasmid DNA recombination have remained unchanged.
Agnieszka M. Murakami, Manabu Yonekura, Katsuhiro Nagatomo, Yasutaka Niwa, Shirou Itagaki, Manabu Murakami   +5 more
doaj   +1 more source

The use of apolipoprotein A-I as a transport form of the green fluorescent protein GFP gene in rat hepatocytes

Сибирский научный медицинский журнал, 2023
The aim of this study was to investigate the possibility of using apolipoprotein A-I (apo A-I) as a transport form of the green fluorescent protein (GFP) gene into rat hepatocytes.Material and methods.
L. M. Polyakov, D. V. Sumenkova, M. V. Kotova, N. V. Trifonova, R. A. Knyazev   +4 more
doaj   +1 more source

Fast and non-viral method to deliver plasmid DNA to human muscle-tendon graft used in anterior cruciate ligament reconstruction

European Cells & Materials, 2023
Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović, B Markelc, I Matić, A Frischer, A Hacobian, T Jesenko, M Cemazar, H Redl, D Marolt Presen, A Ivković   +9 more
doaj   +1 more source

Co-transfection of Plasmid DNA

BioTechniques, 2013
Georg Hannig, Cordula Jany
doaj   +2 more sources

ApE, A Plasmid Editor: A Freely Available DNA Manipulation and Visualization Program

Frontiers in Bioinformatics, 2022
A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and presenting biologically relevant DNA sequences. ApE provides a flexible framework for annotating a sequence manually or using a user-defined library of features.
M. Davis, E. Jorgensen, Chris J. Myers, Jorgensen ApE, A. Editor   +4 more
semanticscholar   +1 more source

Transcription of plasmid DNA: Influence of plasmid DNA/polyethylenimine complex formation [PDF]

Journal of Controlled Release, 2005
Polyethylenimine (PEI) is one of the most potent non-viral vectors. We have developed a lactosylated PEI (Lac-PEI) to enhance cell-specific transfection and have shown that Lac-PEI is more efficient than unsubstituted PEI for gene transfer into immortalized cystic fibrosis airway epithelial SigmaCFTE29o-cells.
Honoré, I., Grosse, S., Frison, N., Favatier, F., Monsigny, M., Fajac, I.   +5 more
openaire   +2 more sources

The integrity of chemically treated plasmid DNA as a chemical-based choice for prion clearance

Regenerative Therapy, 2020
In regenerative medical products for clinical applications, a major concern is the risk of ruminant-derived materials developing transmissible spongiform encephalopathy (TSE) in the manufacturing process. Because of the risk of TSE causing prion disease,
Nozomi Takada, Yasuharu Niwa, Tomoaki Teshigawara, Kazue Isogai, Hanayuki Okura, Akifumi Matsuyama   +5 more
doaj   +1 more source

Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex [PDF]

Molecular Cell
Kevin Corbett, Eugene Kim, Biswajit Pradhan   +2 more
exaly   +2 more sources

RpoS regulates a novel type of plasmid DNA transfer in Escherichia coli. [PDF]

PLoS ONE, 2012
Spontaneous plasmid transformation of Escherichia coli is independent of the DNA uptake machinery for single-stranded DNA (ssDNA) entry. The one-hit kinetic pattern of plasmid transformation indicates that double-stranded DNA (dsDNA) enters E. coli cells
Yanmei Zhang, Chunyu Shi, Jiafei Yu, Jingjing Ren, Dongchang Sun   +4 more
doaj   +1 more source