Regulatory T cells suppress natural killer cells during plasmid DNA vaccination in mice, blunting the CD8+ T cell immune response by the cytokine TGFbeta. [PDF]
PLoS ONE, 2010 CD4(+)CD25(+) regulatory T cells (Tregs) suppress adaptive T cell-mediated immune responses to self- and foreign-antigens. Tregs may also suppress early innate immune responses to vaccine antigens and might decrease vaccine efficacy. NK and NKT cells are
Kwesi Frimpong-Boateng +2 more
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Endocytosis of DNA-Hsp65 alters the pH of the late endosome/lysosome and interferes with antigen presentation. [PDF]
PLoS ONE, 2007 BACKGROUND: Experimental models using DNA vaccine has shown that this vaccine is efficient in generating humoral and cellular immune responses to a wide variety of DNA-derived antigens.
Ana Paula F Trombone +6 more
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Sending Plasmid DNA by Mail [PDF]
BioTechniques, 2001 Michael W. Mortimer, Michael L. Kahn
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A one-step miniprep for the isolation of plasmid DNA and lambda phage particles. [PDF]
PLoS ONE, 2011 Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format.
George Lezin +4 more
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Journal of Pharmacological Sciences, 2005 In vivo gene transfer with plasmid vector has been applied experimentally and clinically; however, the low level of gene transfer efficiency with plasmid vector is a problem.
Shinji Kuroda +3 more
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Bio-Protocol, 2023 High yield of good quality plasmid DNA from gram -ve bacteria (Agrobacterium tumefaciens, A. rhizogenes, and Rhizobium sp.) and gram +ve bacterium (Bacillus thuringiensis) is difficult. The widely used plasmid extraction kits for Escherichia coli yield a
Preshobha Kodackattumannil +5 more
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Scientific advice on the suitability of data for the assessment of DNA integration into the fish genome of a genetically modified DNA plasmid-based veterinary vaccine [PDF]
EFSA Journal, 2013 Pancreas disease caused by salmonid alphavirus in farmed Atlantic salmon (Salmo salar) leads to high mortality rates post infection and histopathological lesions in several organs. As protection against pancreas disease, Novartis developed a prophylactic
European Food Safety Authority
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Synthetic and Systems Biotechnology, 2019 High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories.
Lorna Suckling +6 more
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Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells. [PDF]
PLoS Genetics, 2009 Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA.
Dawit Kidane +7 more
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Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR. [PDF]
PLoS ONE, 2017 Bacteria can be considered as biological nanofactories that manufacture a cornucopia of bioproducts most notably recombinant proteins. As such, they must perfectly match with appropriate plasmid vectors to ensure successful overexpression of target genes.
Magdalena Plotka +2 more
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