Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA [PDF]
, 2008 Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested.
Abee, T. +5 more
core +1 more source
A one-step miniprep for the isolation of plasmid DNA and lambda phage particles. [PDF]
PLoS ONE, 2011 Plasmid DNA minipreps are fundamental techniques in molecular biology. Current plasmid DNA minipreps use alkali and the anionic detergent SDS in a three-solution format.
George Lezin +4 more
doaj +1 more source
Bio-Protocol, 2023 High yield of good quality plasmid DNA from gram -ve bacteria (Agrobacterium tumefaciens, A. rhizogenes, and Rhizobium sp.) and gram +ve bacterium (Bacillus thuringiensis) is difficult. The widely used plasmid extraction kits for Escherichia coli yield a
Preshobha Kodackattumannil +5 more
doaj +1 more source
Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems [PDF]
, 2013 Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo ...
Redzej, Adam +7 more
core +1 more source
Journal of Pharmacological Sciences, 2005 In vivo gene transfer with plasmid vector has been applied experimentally and clinically; however, the low level of gene transfer efficiency with plasmid vector is a problem.
Shinji Kuroda +3 more
doaj +1 more source
Protective antiviral immune responses to pseudorabies virus induced by DNA vaccination using dimethyldioctadecylammonium bromide as an adjuvant [PDF]
, 2002 To enhance the efficacy of a DNA vaccine against pseudorabies virus (PRV), we evaluated the adjuvant properties of plasmids coding for gamma interferon or interleukin-12, of CpG immunostimulatory motifs, and of the conventional adjuvants ...
Eddie G. te Lintelo +23 more
core +1 more source
Scientific advice on the suitability of data for the assessment of DNA integration into the fish genome of a genetically modified DNA plasmid-based veterinary vaccine [PDF]
EFSA Journal, 2013 Pancreas disease caused by salmonid alphavirus in farmed Atlantic salmon (Salmo salar) leads to high mortality rates post infection and histopathological lesions in several organs. As protection against pancreas disease, Novartis developed a prophylactic
European Food Safety Authority
doaj +1 more source
Bacteria spring a surprise [PDF]
, 2014 Cell division involves the precise duplication of all the genetic material in the cell—both the chromosomes and the plasmid DNA—and the equal division of this material between the two daughter cells.
Srinivasan, Rajagopalan +3 more
core +1 more source
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells. [PDF]
PLoS Genetics, 2009 Cytological and genetic evidence suggests that the Bacillus subtilis DNA uptake machinery localizes at a single cell pole and takes up single-stranded (ss) DNA.
Dawit Kidane +7 more
doaj +1 more source
Mathematical and experimental approaches to the dimer catastrophe theory [PDF]
, 2011 Multicopy plasmids rely on random distribution for stable inheritance by daughter cells at division. Threats to plasmid copy number increase the probability of plasmid loss, which can be detrimental to both plasmid and host. Plasmid dimers emerge through
core +3 more sources