Rapid method for plasmid DNA recombination (Murakami-system)
MethodsX, 2023 DNA recombination is a useful technology for cloning and subsequent functional analysis, while standard techniques for plasmid DNA recombination have remained unchanged.
Agnieszka M. Murakami +5 more
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European Cells & Materials, 2023 Muscle tissues attached to the proximal ends of hamstring tendon grafts are routinely discarded during anterior cruciate ligament (ACL) reconstruction. Preserving the muscle on the ACL graft was shown to improve the surgery outcomes, possibly due to the ...
A Šećerović +9 more
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Transcription of plasmid DNA: Influence of plasmid DNA/polyethylenimine complex formation [PDF]
Journal of Controlled Release, 2005 Polyethylenimine (PEI) is one of the most potent non-viral vectors. We have developed a lactosylated PEI (Lac-PEI) to enhance cell-specific transfection and have shown that Lac-PEI is more efficient than unsubstituted PEI for gene transfer into immortalized cystic fibrosis airway epithelial SigmaCFTE29o-cells.
Honoré, I. +5 more
openaire +2 more sources
Quantification of complex DNA damage by ionising radiation: An experimental and theoretical approach [PDF]
, 2000 This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Ionising radiation potentially produces a broad spectrum of damage in DNA including single and double strand breaks (ssb and dsb) and base damages.
Fulford, Jonathan
core +7 more sources
RpoS regulates a novel type of plasmid DNA transfer in Escherichia coli. [PDF]
PLoS ONE, 2012 Spontaneous plasmid transformation of Escherichia coli is independent of the DNA uptake machinery for single-stranded DNA (ssDNA) entry. The one-hit kinetic pattern of plasmid transformation indicates that double-stranded DNA (dsDNA) enters E. coli cells
Yanmei Zhang +4 more
doaj +1 more source
The integrity of chemically treated plasmid DNA as a chemical-based choice for prion clearance
Regenerative Therapy, 2020 In regenerative medical products for clinical applications, a major concern is the risk of ruminant-derived materials developing transmissible spongiform encephalopathy (TSE) in the manufacturing process. Because of the risk of TSE causing prion disease,
Nozomi Takada +5 more
doaj +1 more source
Regulatory T cells suppress natural killer cells during plasmid DNA vaccination in mice, blunting the CD8+ T cell immune response by the cytokine TGFbeta. [PDF]
PLoS ONE, 2010 CD4(+)CD25(+) regulatory T cells (Tregs) suppress adaptive T cell-mediated immune responses to self- and foreign-antigens. Tregs may also suppress early innate immune responses to vaccine antigens and might decrease vaccine efficacy. NK and NKT cells are
Kwesi Frimpong-Boateng +2 more
doaj +1 more source
Multiple pathways of plasmid DNA transfer in Helicobacter pylori [PDF]
, 2012 Many Helicobacter pylori (Hp) strains carry cryptic plasmids of different size and gene content, the function of which is not well understood. A subgroup of these plasmids (e.g.
Haas, Rainer +17 more
core +2 more sources
Endocytosis of DNA-Hsp65 alters the pH of the late endosome/lysosome and interferes with antigen presentation. [PDF]
PLoS ONE, 2007 BACKGROUND: Experimental models using DNA vaccine has shown that this vaccine is efficient in generating humoral and cellular immune responses to a wide variety of DNA-derived antigens.
Ana Paula F Trombone +6 more
doaj +1 more source
Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences [PDF]
, 1987 Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication.
Zabel, Pim +11 more
core +2 more sources