Results 61 to 70 of about 2,326,635 (307)

Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin

Emerging Microbes and Infections, 2018
Plasmid DNA encoding flagellin FlaB3 was used as a vaccination candidate for the evaluation of immunogenicity and protection against Treponema pallidum subsp. pallidum dissemination.
Kang Zheng, Man Xu, Yongjian Xiao, Haodang Luo, Yafeng Xie, Jian Yu, Manyi Tan, Yumeng Li, Feijun Zhao, Tiebing Zeng, Yimou Wu   +10 more
doaj   +1 more source

Characterizing DNA condensation and conformational changes in organic solvents. [PDF]

PLoS ONE, 2010
Organic solvents offer a new approach to formulate DNA into novel structures suitable for gene delivery. In this study, we examined the in situ behavior of DNA in N, N-dimethylformamide (DMF) at low concentration via laser light scattering (LLS), TEM, UV
Fuyou Ke, Yen Kim Luu, Michael Hadjiargyrou, Dehai Liang   +3 more
doaj   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

The plasmid-stabilizing Ytl2 protein coats DNA in a sequence-independent manner

, 2001
Plasmids carrying the ytl2 gene from the large resident plasmid pSLT of Salmonella typhimurium were stabilized >10 5-fold (compared to control ytl2-free plasmids) in S. typhimurium cells.
Morris, C, Hackett, J, Hackett, J., Wong, DKH, Morris, C.   +4 more
core   +1 more source

Correlation of Antibiotic Resistance and Restriction Mapping of Plasmid DNA Isolated from E. coli Causing Urinary Tract Infection

Journal of Pure and Applied Microbiology, 2019
Pathogenic bacteria are constantly adapted against antimicrobial drugs by arising new traits of drug resistance. The genes of such resistance are mostly possessed by pathogens due to the random use of antibiotics and commonly held on plasmid DNA ...
Mohammed Jasim Al-Shamart, Muslim Idan Mohsin   +1 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Influence of DNA characteristics on cell membrane damage stimulated by electrical short-circuiting via a low-conductive aqueous droplet in dielectric oil.

PLoS ONE, 2023
We investigated gene electrotransfer using electrical short-circuiting via a cell suspension droplet in dielectric oil. An aqueous droplet of a few microliters placed between a pair of electrodes can be deformed by an intense DC electric field depending ...
Yoshino Tsurusaki, Yuki Watanabe, Rika Numano, Takayuki Shibata, Hirofumi Kurita   +4 more
doaj   +1 more source

An investigation of horizontal transfer of feed introduced DNA to the aerobic microbiota of the gastrointestinal tract of rats [PDF]

, 2012
BACKGROUND: Horizontal gene transfer through natural transformation of members of the microbiota of the lower gastrointestinal tract (GIT) of mammals has not yet been described.
Raddadi Noura, Nielsen KM, Traavik T, Terje Traavik, Brusetti Lorenzo, Averhoff, Beate, Nordgård Lise, Beate Averhoff, Nordgård, Lise, Noura Raddadi, Brusetti L, Nielsen, Kaare Magne, Kaare Magne Nielsen, Traavik, Terje, Nielsen Kaare, Traavik Terje, Lorenzo Brusetti, Raddadi, Noura, Averhoff B, Brusetti, Lorenzo, Averhoff Beate, Nordgård L, Lise Nordgard   +22 more
core   +1 more source

An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]

, 2008
Background: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Liu Huanting, James H Naismith, Liu, Huanting, Naismith, James Henderson, Naismith James H, Huanting Liu   +5 more
core   +2 more sources